Development of tools for studying Olea europaea: Pseudomonas savastanoi interaction

Detalhes bibliográficos
Autor(a) principal: Cruz, A. Braga da
Data de Publicação: 2002
Outros Autores: Tavares, R. M.
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/1822/3365
Resumo: Poster apresentado no 13th Congress of the Federation of European Societies of Plant Physiology, em 2002, Hersonissos, Creta, Grécia
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spelling Development of tools for studying Olea europaea: Pseudomonas savastanoi interactionPoster apresentado no 13th Congress of the Federation of European Societies of Plant Physiology, em 2002, Hersonissos, Creta, GréciaPortugal is one of the main world olive oil producers. Consequently, the culture of olive trees is of considerable importance for our country. In order to maintain and improve our varieties, responses to environmental stresses and pests, which can seriously affect productivity, must be studied, and clones more adapted to challenge stress conditions must be found. One problem that seriously affects Portuguese olive orchards' is olive knot, a disease caused by Pseudomonas savastanoi (Pseudomonas syringae pv savastanoi) which, causing tumours in the stems and leaves of the trees, drastically reduces the production of fruits. Some varieties, like Galega Vulgar, are know to be resistant to the disease, while others, like Cordovil de Serpa, variety are know to be susceptible. With the objective of developing an in vitro system of eliciation of Olea europaea with an avirulent strain of Pseudomonas savastanoi, we have initiated suspension cell cultures of from calli of the variety Galega Vulgar. Cells in middle exponential growth phase were incubated with a suspension of P. savastanoi.Hypersensitive response was studied using XTT (sodium,3’-(1-[phenylamino-carbonyl]-3,4-tetrazolium)-bis(4-methoxy-6-nitro) benzene-sulfonic acid) by the quantification of the perhydroxyl / superoxide (H2O./O2-) radical acid-base pair associated to the oxidative burst, during the time course of elicitation. The results showed the existence of two bursts (100 and 300 min after eliciation), which are characteristic of the HR that occurs in the incompatible interactions). By screening an elicited O. europaea cDNA library a clone encoding phenylalanine ammonia lyase (PAL) was obtained. The coding region was used as probe for evaluate Pal expression levels. The results will be discussed by comparing to variation of PAL activity, during the time course of elicitation.Fundação para a Ciência e a Tecnologia (FCT) – grant ref. SFRH/BD/6654/2001, Programa Operacional “Ciência, Tecnologia, Inovação” (POCTI) - POCTI / AGR / 36239 / 99-00.Universidade do MinhoCruz, A. Braga daTavares, R. M.2002-092002-09-01T00:00:00Zconference posterinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/3365enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T05:02:26Zoai:repositorium.sdum.uminho.pt:1822/3365Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:06:21.903054Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Development of tools for studying Olea europaea: Pseudomonas savastanoi interaction
title Development of tools for studying Olea europaea: Pseudomonas savastanoi interaction
spellingShingle Development of tools for studying Olea europaea: Pseudomonas savastanoi interaction
Cruz, A. Braga da
title_short Development of tools for studying Olea europaea: Pseudomonas savastanoi interaction
title_full Development of tools for studying Olea europaea: Pseudomonas savastanoi interaction
title_fullStr Development of tools for studying Olea europaea: Pseudomonas savastanoi interaction
title_full_unstemmed Development of tools for studying Olea europaea: Pseudomonas savastanoi interaction
title_sort Development of tools for studying Olea europaea: Pseudomonas savastanoi interaction
author Cruz, A. Braga da
author_facet Cruz, A. Braga da
Tavares, R. M.
author_role author
author2 Tavares, R. M.
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Cruz, A. Braga da
Tavares, R. M.
description Poster apresentado no 13th Congress of the Federation of European Societies of Plant Physiology, em 2002, Hersonissos, Creta, Grécia
publishDate 2002
dc.date.none.fl_str_mv 2002-09
2002-09-01T00:00:00Z
dc.type.driver.fl_str_mv conference poster
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/3365
url http://hdl.handle.net/1822/3365
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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