Influence of organic solvents on cutinase stability and accessibility to polyamide fibers

Bibliographic Details
Main Author: Silva, Carla Manuela Pereira Marinho da
Publication Date: 2005
Other Authors: Matamá, Maria Teresa, Gübitz, Georg M., Paulo, Artur Cavaco
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/2792
Summary: ABSTRACT: The stability of cutinase was measured in the presence of imethylacetamide (DMA) and benzyl alcohol (BA). These solvents are known swelling agents of polyamide fibers. We found that cutinase retains some stability (half-life of 14 h), enough for an enzymatic treatment with a solvent composition of 1.5% DMA þ 10% BA. Enzymatic treatment in the presence of these solvent compositions yields more soluble amino groups as well as more amino end groups at the fiber level. We have shown evidence that the presence of minor quantities of solvent can increase the accessibility of the enzyme towards the polyamide fiber.
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spelling Influence of organic solvents on cutinase stability and accessibility to polyamide fibersAminesCutinaseHydrolysisPolyamide 6.6SolventsScience & TechnologyABSTRACT: The stability of cutinase was measured in the presence of imethylacetamide (DMA) and benzyl alcohol (BA). These solvents are known swelling agents of polyamide fibers. We found that cutinase retains some stability (half-life of 14 h), enough for an enzymatic treatment with a solvent composition of 1.5% DMA þ 10% BA. Enzymatic treatment in the presence of these solvent compositions yields more soluble amino groups as well as more amino end groups at the fiber level. We have shown evidence that the presence of minor quantities of solvent can increase the accessibility of the enzyme towards the polyamide fiber.WileyUniversidade do MinhoSilva, Carla Manuela Pereira Marinho daMatamá, Maria TeresaGübitz, Georg M.Paulo, Artur Cavaco20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2792eng“Journal of Polymer Science: Part A: Polymer Chemistry”. ISSN 0887-624X. 43 (2005) 2749–2753.0887-624X10.1002/pola.20739info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T04:56:13Zoai:repositorium.sdum.uminho.pt:1822/2792Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:03:01.876389Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Influence of organic solvents on cutinase stability and accessibility to polyamide fibers
title Influence of organic solvents on cutinase stability and accessibility to polyamide fibers
spellingShingle Influence of organic solvents on cutinase stability and accessibility to polyamide fibers
Silva, Carla Manuela Pereira Marinho da
Amines
Cutinase
Hydrolysis
Polyamide 6.6
Solvents
Science & Technology
title_short Influence of organic solvents on cutinase stability and accessibility to polyamide fibers
title_full Influence of organic solvents on cutinase stability and accessibility to polyamide fibers
title_fullStr Influence of organic solvents on cutinase stability and accessibility to polyamide fibers
title_full_unstemmed Influence of organic solvents on cutinase stability and accessibility to polyamide fibers
title_sort Influence of organic solvents on cutinase stability and accessibility to polyamide fibers
author Silva, Carla Manuela Pereira Marinho da
author_facet Silva, Carla Manuela Pereira Marinho da
Matamá, Maria Teresa
Gübitz, Georg M.
Paulo, Artur Cavaco
author_role author
author2 Matamá, Maria Teresa
Gübitz, Georg M.
Paulo, Artur Cavaco
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, Carla Manuela Pereira Marinho da
Matamá, Maria Teresa
Gübitz, Georg M.
Paulo, Artur Cavaco
dc.subject.por.fl_str_mv Amines
Cutinase
Hydrolysis
Polyamide 6.6
Solvents
Science & Technology
topic Amines
Cutinase
Hydrolysis
Polyamide 6.6
Solvents
Science & Technology
description ABSTRACT: The stability of cutinase was measured in the presence of imethylacetamide (DMA) and benzyl alcohol (BA). These solvents are known swelling agents of polyamide fibers. We found that cutinase retains some stability (half-life of 14 h), enough for an enzymatic treatment with a solvent composition of 1.5% DMA þ 10% BA. Enzymatic treatment in the presence of these solvent compositions yields more soluble amino groups as well as more amino end groups at the fiber level. We have shown evidence that the presence of minor quantities of solvent can increase the accessibility of the enzyme towards the polyamide fiber.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/2792
url http://hdl.handle.net/1822/2792
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv “Journal of Polymer Science: Part A: Polymer Chemistry”. ISSN 0887-624X. 43 (2005) 2749–2753.
0887-624X
10.1002/pola.20739
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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