Quantification of trans-resveratrol-loaded solid lipid nanoparticles by a validated reverse-phase HPLC photodiode array

• Autor(a) principal: Rigon, Roberta B.
• Data de Publicação: 2019
• Outros Autores: Fachinetti, Naiara, Severino, Patrí­cia, Durazzo, Alessandra, Lucarini, Massimo, Atanasov, Atanas G., El Mamouni, Soukaina, Chorilli, Marlus, Santini, Antonello, Souto, Eliana B.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
• Texto Completo: http://hdl.handle.net/1822/62227
• topic: trans-resveratrol; nanotechnology; solid lipid nanoparticles; high-performance liquid chromatography; Science & Technology

A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 03.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.65.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.910 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1100 µg/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 ± 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release.