Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract

Bibliographic Details
Main Author: André, Rebeca
Publication Date: 2024
Other Authors: Pacheco, Rita, Santos, Hugo M., Serralheiro, Maria Luísa
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/171885
Summary: PM003/2016). Support was also provided by Bioisi MassSpec Facility. Publisher Copyright: © 2024 by the authors.
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spelling Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus ExtractOmic Insights into Molecular Mechanisms at the Intestinal LevelCaco-2 cell lineFucus vesiculosusmetabolomicsNPC1proteomicsPharmaceutical ScienceDrug DiscoveryPharmacology, Toxicology and Pharmaceutics (miscellaneous)SDG 3 - Good Health and Well-beingPM003/2016). Support was also provided by Bioisi MassSpec Facility. Publisher Copyright: © 2024 by the authors.High blood cholesterol levels are a major risk factor for cardiovascular diseases. A purified aqueous extract of Fucus vesiculosus, rich in phlorotannins and peptides, has been described for its potential to inhibit cholesterol biosynthesis and intestinal absorption. In this work, the effect of this extract on intestinal cells’ metabolites and proteins was analysed to gain a deeper understanding of its mode of action on lipids’ metabolism, particularly concerning the absorption and transport of exogenous cholesterol. Caco-2 cells, differentiated into enterocytes, were exposed to the extract, and analysed by untargeted metabolomics and proteomics. The results of the metabolomic analysis showed statistically significant differences in glutathione content of cells exposed to the extract compared to control cells, along with an increased expression of fatty acid amides in exposed cells. A proteomic analysis showed an increased expression in cells exposed to the extract compared to control cells of FAB1 and NPC1, proteins known to be involved in lipid metabolism and transport. To the extent of our knowledge, this study is the first use of untargeted metabolomics and a proteomic analysis to investigate the effects of F. vesiculosus on differentiated Caco-2 cells, offering insights into the molecular mechanism of the extract’s compounds on intestinal cells.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNAndré, RebecaPacheco, RitaSantos, Hugo M.Serralheiro, Maria Luísa2024-09-16T22:20:27Z2024-04-202024-04-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/10362/171885eng1660-3397PURE: 98898810https://doi.org/10.3390/md22040187info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-12-30T01:34:34Zoai:run.unl.pt:10362/171885Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T18:53:24.918159Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract
Omic Insights into Molecular Mechanisms at the Intestinal Level
title Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract
spellingShingle Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract
André, Rebeca
Caco-2 cell line
Fucus vesiculosus
metabolomics
NPC1
proteomics
Pharmaceutical Science
Drug Discovery
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
SDG 3 - Good Health and Well-being
title_short Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract
title_full Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract
title_fullStr Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract
title_full_unstemmed Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract
title_sort Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract
author André, Rebeca
author_facet André, Rebeca
Pacheco, Rita
Santos, Hugo M.
Serralheiro, Maria Luísa
author_role author
author2 Pacheco, Rita
Santos, Hugo M.
Serralheiro, Maria Luísa
author2_role author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv André, Rebeca
Pacheco, Rita
Santos, Hugo M.
Serralheiro, Maria Luísa
dc.subject.por.fl_str_mv Caco-2 cell line
Fucus vesiculosus
metabolomics
NPC1
proteomics
Pharmaceutical Science
Drug Discovery
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
SDG 3 - Good Health and Well-being
topic Caco-2 cell line
Fucus vesiculosus
metabolomics
NPC1
proteomics
Pharmaceutical Science
Drug Discovery
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
SDG 3 - Good Health and Well-being
description PM003/2016). Support was also provided by Bioisi MassSpec Facility. Publisher Copyright: © 2024 by the authors.
publishDate 2024
dc.date.none.fl_str_mv 2024-09-16T22:20:27Z
2024-04-20
2024-04-20T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/171885
url http://hdl.handle.net/10362/171885
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1660-3397
PURE: 98898810
https://doi.org/10.3390/md22040187
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14
application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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