Dexamethasone-loaded carboxymethylchitosan/poly(amidoamine) dendrimer nanoparticles enhances bone formation in vivo

• Main Author: Oliveira, J. M.
• Publication Date: 2008
• Other Authors: Sousa, R. A., Kotobuki, N., Malafaya, P. B., Hirose, M., Mano, J. F., Reis, R. L., Ohgushi, H.
• Language: eng
• Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
• Download full: http://hdl.handle.net/1822/58599
• topic: bone; Carboxymethylchitosan/poly(amidoamine) dendrimer; Nanoparticles; Science & Technology

[Excerpt] Dexamethasone-loaded carboxymethylchitosan/poly(amidoamine) dendrimer nanoparticles, CMC/PAMAM-Dex were successfully synthesized to find applications as a controlled system of relevant molecules in Bone Tissue Engineering. These are aimed at modulatingtheproliferation anddifferentiationofstem cells,both invitro and in vivo. In previous work, we have demonstrated that CMC/ PAMAM-Dex nanoparticles are internalized with high efficiency by different cell types, namely osteoblastic-cells, SaOs-2 and rat bone marrow stromal cells, RBMSCs. The biocompatibility of HA and SPCL scaffolds was also assessed by means of seeding RBMSCs onto the materials and performing a luminescent cell viability assay, after 24 and 72hrs. [...]