Long-Term Hypercaloric Diet Consumption Exacerbates Age-Induced Dysmetabolism and Carotid Body Dysfunction

Bibliographic Details
Main Author: Melo, Bernardete F
Publication Date: 2022
Other Authors: Sacramento, Joana F, Capucho, Adriana M, Sampaio-Pires, Dinis, Prego, Cláudia S, V Conde, Silvia
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/141514
Summary: Funding: This study was supported by the Portuguese Foundation for Science with a research grant EXPL/MED-NEU/0733/2021 and a contract for JS (CEEC IND/02428/2018).
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spelling Long-Term Hypercaloric Diet Consumption Exacerbates Age-Induced Dysmetabolism and Carotid Body DysfunctionBeneficial Effects of CSN Denervationcarotid bodymetabolic diseaseageinghypercaloric dietscarotid sinus nerveinsulin receptorFunding: This study was supported by the Portuguese Foundation for Science with a research grant EXPL/MED-NEU/0733/2021 and a contract for JS (CEEC IND/02428/2018).Carotid bodies (CBs) are metabolic sensors whose dysfunction is involved in the genesis of dysmetabolic states. Ageing induces significant alterations in CB function also prompting to metabolic deregulation. On the other hand, metabolic disease can accelerate ageing processes. Taking these into account, we evaluated the effect of long-term hypercaloric diet intake and CSN resection on age-induced dysmetabolism and CB function. Experiments were performed in male Wistar rats subjected to 14 or 44 weeks of high-fat high-sucrose (HFHSu) or normal chow (NC) diet and subjected to either carotid sinus nerve (CSN) resection or a sham procedure. After surgery, the animals were kept on a diet for more than 9 weeks. Metabolic parameters, basal ventilation, and hypoxic and hypercapnic ventilatory responses were evaluated. CB type I and type II cells, HIF-1α and insulin receptor (IR), and GLP-1 receptor (GLP1-R)-positive staining were analyzed by immunofluorescence. Ageing decreased by 61% insulin sensitivity in NC animals, without altering glucose tolerance. Short-term and long-term HFHSu intake decreased insulin sensitivity by 55 and 62% and glucose tolerance by 8 and 29%, respectively. CSN resection restored insulin sensitivity and glucose tolerance. Ageing decreased spontaneous ventilation, but short-term or long-term intake of HFHSu diet and CSN resection did not modify basal ventilatory parameters. HFHSu diet increased hypoxic ventilatory responses in young and adult animals, effects attenuated by CSN resection. Ageing, hypercaloric diet, and CSN resection did not change hypercapnic ventilatory responses. Adult animals showed decreased type I cells and IR and GLP-1R staining without altering the number of type II cells and HIF-1α. HFHSu diet increased the number of type I and II cells and IR in young animals without significantly changing these values in adult animals. CSN resection restored the number of type I cells in HFHSu animals and decreased IR-positive staining in all the groups of animals, without altering type II cells, HIF-1α, or GLP-1R staining. In conclusion, long-term hypercaloric diet consumption exacerbates age-induced dysmetabolism, and both short- and long-term hypercaloric diet intakes promote significant alterations in CB function. CSN resection ameliorates these effects. We suggest that modulation of CB activity is beneficial in exacerbated stages of dysmetabolism.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNMelo, Bernardete FSacramento, Joana FCapucho, Adriana MSampaio-Pires, DinisPrego, Cláudia SV Conde, Silvia2022-07-06T22:35:12Z2022-05-042022-05-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/141514eng1664-042XPURE: 45176813https://doi.org/10.3389/fphys.2022.889660info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-05-26T01:40:17Zoai:run.unl.pt:10362/141514Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:34:12.302347Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Long-Term Hypercaloric Diet Consumption Exacerbates Age-Induced Dysmetabolism and Carotid Body Dysfunction
Beneficial Effects of CSN Denervation
title Long-Term Hypercaloric Diet Consumption Exacerbates Age-Induced Dysmetabolism and Carotid Body Dysfunction
spellingShingle Long-Term Hypercaloric Diet Consumption Exacerbates Age-Induced Dysmetabolism and Carotid Body Dysfunction
Melo, Bernardete F
carotid body
metabolic disease
ageing
hypercaloric diets
carotid sinus nerve
insulin receptor
title_short Long-Term Hypercaloric Diet Consumption Exacerbates Age-Induced Dysmetabolism and Carotid Body Dysfunction
title_full Long-Term Hypercaloric Diet Consumption Exacerbates Age-Induced Dysmetabolism and Carotid Body Dysfunction
title_fullStr Long-Term Hypercaloric Diet Consumption Exacerbates Age-Induced Dysmetabolism and Carotid Body Dysfunction
title_full_unstemmed Long-Term Hypercaloric Diet Consumption Exacerbates Age-Induced Dysmetabolism and Carotid Body Dysfunction
title_sort Long-Term Hypercaloric Diet Consumption Exacerbates Age-Induced Dysmetabolism and Carotid Body Dysfunction
author Melo, Bernardete F
author_facet Melo, Bernardete F
Sacramento, Joana F
Capucho, Adriana M
Sampaio-Pires, Dinis
Prego, Cláudia S
V Conde, Silvia
author_role author
author2 Sacramento, Joana F
Capucho, Adriana M
Sampaio-Pires, Dinis
Prego, Cláudia S
V Conde, Silvia
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Melo, Bernardete F
Sacramento, Joana F
Capucho, Adriana M
Sampaio-Pires, Dinis
Prego, Cláudia S
V Conde, Silvia
dc.subject.por.fl_str_mv carotid body
metabolic disease
ageing
hypercaloric diets
carotid sinus nerve
insulin receptor
topic carotid body
metabolic disease
ageing
hypercaloric diets
carotid sinus nerve
insulin receptor
description Funding: This study was supported by the Portuguese Foundation for Science with a research grant EXPL/MED-NEU/0733/2021 and a contract for JS (CEEC IND/02428/2018).
publishDate 2022
dc.date.none.fl_str_mv 2022-07-06T22:35:12Z
2022-05-04
2022-05-04T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/141514
url http://hdl.handle.net/10362/141514
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-042X
PURE: 45176813
https://doi.org/10.3389/fphys.2022.889660
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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