Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry

Bibliographic Details
Main Author: Andrade, Joana Catarina
Publication Date: 2020
Other Authors: Joao, Antonio Lopes, Alonso, Carlos de Sousa, BARRETO, ANTONIO, Henriques, Ana Rita
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.5/24099
Summary: Research Areas: Infectious Diseases ; Pharmacology & Pharmacy
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spelling Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industryBiocideListeria monocytogenesBiofilmPlanktonic culturePulsed-field gel electrophoresisResearch Areas: Infectious Diseases ; Pharmacology & PharmacyListeria monocytogenes is a foodborne pathogen of special concern for ready-to-eat food producers. The control of its presence is a critical step in which food-grade sanitizers play an essential role. L. monocytogenes is believed to persist in food processing environments in biofilms, exhibiting less susceptibility to sanitizers than planktonic cells. This study aimed to test the susceptibility of L. monocytogenes in planktonic culture and biofilm to three commercial food-grade sanitizers and to benzalkonium chloride; together with the genetic subtyping of the isolates. L. monocytogenes isolates were collected from raw materials, final products and food-contact surfaces during a 6-year period from a ready-to-eat meat-producing food industry and genetically characterized. Serogrouping and pulsed-field gel electrophoresis (PFGE) revealed genetic variability and di erentiated L. monocytogenes isolates in three clusters. The biofilm-forming ability assay revealed that the isolates were weak biofilm producers. L. monocytogenes strains were susceptible both in the planktonic and biofilm form to oxidizing and ethanol-based compounds and to benzalkonium chloride, but not to quaternary ammonium compound. A positive association of biofilm-forming ability and LD90 values for quaternary ammonium compound and benzalkonium chloride was found. This study highlights the need for preventive measures improvement and for a conscious selection and use of sanitizers in food-related environments to control Listeria monocytogenes.MDPIRepositório da Universidade de LisboaAndrade, Joana CatarinaJoao, Antonio LopesAlonso, Carlos de SousaBARRETO, ANTONIOHenriques, Ana Rita2022-04-18T14:54:32Z2020-07-162020-07-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24099engAndrade JC, Joao AL, Alonso CD, Barreto AS, Henriques AR. 2020. Genetic Subtyping, Biofilm-Forming Ability and Biocide Susceptibility of Listeria monocytogenes Strains Isolated from a Ready-to-Eat Food Industry, Antibiotics, 9(7):416. Doi 10.3390/antibiotics90704162079-638210.3390/antibiotics9070416info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T15:24:38Zoai:repositorio.ulisboa.pt:10400.5/24099Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:42:27.524292Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry
title Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry
spellingShingle Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry
Andrade, Joana Catarina
Biocide
Listeria monocytogenes
Biofilm
Planktonic culture
Pulsed-field gel electrophoresis
title_short Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry
title_full Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry
title_fullStr Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry
title_full_unstemmed Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry
title_sort Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry
author Andrade, Joana Catarina
author_facet Andrade, Joana Catarina
Joao, Antonio Lopes
Alonso, Carlos de Sousa
BARRETO, ANTONIO
Henriques, Ana Rita
author_role author
author2 Joao, Antonio Lopes
Alonso, Carlos de Sousa
BARRETO, ANTONIO
Henriques, Ana Rita
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Andrade, Joana Catarina
Joao, Antonio Lopes
Alonso, Carlos de Sousa
BARRETO, ANTONIO
Henriques, Ana Rita
dc.subject.por.fl_str_mv Biocide
Listeria monocytogenes
Biofilm
Planktonic culture
Pulsed-field gel electrophoresis
topic Biocide
Listeria monocytogenes
Biofilm
Planktonic culture
Pulsed-field gel electrophoresis
description Research Areas: Infectious Diseases ; Pharmacology & Pharmacy
publishDate 2020
dc.date.none.fl_str_mv 2020-07-16
2020-07-16T00:00:00Z
2022-04-18T14:54:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/24099
url http://hdl.handle.net/10400.5/24099
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Andrade JC, Joao AL, Alonso CD, Barreto AS, Henriques AR. 2020. Genetic Subtyping, Biofilm-Forming Ability and Biocide Susceptibility of Listeria monocytogenes Strains Isolated from a Ready-to-Eat Food Industry, Antibiotics, 9(7):416. Doi 10.3390/antibiotics9070416
2079-6382
10.3390/antibiotics9070416
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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