Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies

Detalhes bibliográficos
Autor(a) principal: International Society for Extracellular Vesicles Cerebrospinal Fluid Task Force
Data de Publicação: 2024
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10362/182730
Resumo: Funding Information: The authors are grateful to the respondents of the ISEV CSF Task Force survey, the time and efforts of the Task Force team members, and the members who provided feedback and edits for the final manuscript. This work was funded by NIH RF1 AG059392 (JAS), OHSU Center for Women's Health Circle of Giving (JAS, USS), R01 AG079356 (JAS); NIH RF1 AG054199 (TI), R01 AG066429 (TI), R01 AG067763 (TI); Fundação para a Ciência e a Tecnologia, I.P., through iNOVA4Health (UIDB/04462/2020, UIDP/04462/2020) and the LS4FUTURE Associated Laboratory (LA/P/0087/2020) (JC). Funding Information: The authors are grateful to the respondents of the ISEV CSF Task Force survey, the time and efforts of the Task Force team members, and the members who provided feedback and edits for the final manuscript. This work was funded by NIH RF1 AG059392 (JAS), OHSU Center for Women's Health Circle of Giving (JAS, USS), R01 AG079356 (JAS); NIH RF1 AG054199 (TI), R01 AG066429 (TI), R01 AG067763 (TI); Fundação para a Ciência e a Tecnologia, I.P., through iNOVA4Health (UIDB/04462/2020, UIDP/04462/2020) and the LS4FUTURE Associated Laboratory (LA/P/0087/2020) (JC). Publisher Copyright: © 2023 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.
id RCAP_5246c43b0b9eb7e4db083854ab19e130
oai_identifier_str oai:run.unl.pt:10362/182730
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studiesbiomarkersbraincentral nervous systemcerebrospinal fluidexosomeextracellular vesiclerecommendations and reportingHistologyCell BiologyFunding Information: The authors are grateful to the respondents of the ISEV CSF Task Force survey, the time and efforts of the Task Force team members, and the members who provided feedback and edits for the final manuscript. This work was funded by NIH RF1 AG059392 (JAS), OHSU Center for Women's Health Circle of Giving (JAS, USS), R01 AG079356 (JAS); NIH RF1 AG054199 (TI), R01 AG066429 (TI), R01 AG067763 (TI); Fundação para a Ciência e a Tecnologia, I.P., through iNOVA4Health (UIDB/04462/2020, UIDP/04462/2020) and the LS4FUTURE Associated Laboratory (LA/P/0087/2020) (JC). Funding Information: The authors are grateful to the respondents of the ISEV CSF Task Force survey, the time and efforts of the Task Force team members, and the members who provided feedback and edits for the final manuscript. This work was funded by NIH RF1 AG059392 (JAS), OHSU Center for Women's Health Circle of Giving (JAS, USS), R01 AG079356 (JAS); NIH RF1 AG054199 (TI), R01 AG066429 (TI), R01 AG067763 (TI); Fundação para a Ciência e a Tecnologia, I.P., through iNOVA4Health (UIDB/04462/2020, UIDP/04462/2020) and the LS4FUTURE Associated Laboratory (LA/P/0087/2020) (JC). Publisher Copyright: © 2023 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.Cerebrospinal fluid (CSF) is a clear, transparent fluid derived from blood plasma that protects the brain and spinal cord against mechanical shock, provides buoyancy, clears metabolic waste and transports extracellular components to remote sites in the brain. Given its contact with the brain and the spinal cord, CSF is the most informative biofluid for studies of the central nervous system (CNS). In addition to other components, CSF contains extracellular vesicles (EVs) that carry bioactive cargoes (e.g., lipids, nucleic acids, proteins), and that can have biological functions within and beyond the CNS. Thus, CSF EVs likely serve as both mediators of and contributors to communication in the CNS. Accordingly, their potential as biomarkers for CNS diseases has stimulated much excitement for and attention to CSF EV research. However, studies on CSF EVs present unique challenges relative to EV studies in other biofluids, including the invasive nature of CSF collection, limited CSF volumes and the low numbers of EVs in CSF as compared to plasma. Here, the objectives of the International Society for Extracellular Vesicles CSF Task Force are to promote the reproducibility of CSF EV studies by providing current reporting and best practices, and recommendations and reporting guidelines, for CSF EV studies. To accomplish this, we created and distributed a world-wide survey to ISEV members to assess methods considered ‘best practices’ for CSF EVs, then performed a detailed literature review for CSF EV publications that was used to curate methods and resources. Based on responses to the survey and curated information from publications, the CSF Task Force herein provides recommendations and reporting guidelines to promote the reproducibility of CSF EV studies in seven domains: (i) CSF Collection, Processing, and Storage; (ii) CSF EV Separation/Concentration; (iii) CSF EV Size and Number Measurements; (iv) CSF EV Protein Studies; (v) CSF EV RNA Studies; (vi) CSF EV Omics Studies and (vii) CSF EV Functional Studies.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNInternational Society for Extracellular Vesicles Cerebrospinal Fluid Task Force2025-05-07T21:16:17Z2024-012024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/182730eng2001-3078PURE: 83669663https://doi.org/10.1002/jev2.12397info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-05-12T01:46:56Zoai:run.unl.pt:10362/182730Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T07:13:45.817868Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies
title Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies
spellingShingle Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies
International Society for Extracellular Vesicles Cerebrospinal Fluid Task Force
biomarkers
brain
central nervous system
cerebrospinal fluid
exosome
extracellular vesicle
recommendations and reporting
Histology
Cell Biology
title_short Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies
title_full Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies
title_fullStr Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies
title_full_unstemmed Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies
title_sort Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies
author International Society for Extracellular Vesicles Cerebrospinal Fluid Task Force
author_facet International Society for Extracellular Vesicles Cerebrospinal Fluid Task Force
author_role author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv International Society for Extracellular Vesicles Cerebrospinal Fluid Task Force
dc.subject.por.fl_str_mv biomarkers
brain
central nervous system
cerebrospinal fluid
exosome
extracellular vesicle
recommendations and reporting
Histology
Cell Biology
topic biomarkers
brain
central nervous system
cerebrospinal fluid
exosome
extracellular vesicle
recommendations and reporting
Histology
Cell Biology
description Funding Information: The authors are grateful to the respondents of the ISEV CSF Task Force survey, the time and efforts of the Task Force team members, and the members who provided feedback and edits for the final manuscript. This work was funded by NIH RF1 AG059392 (JAS), OHSU Center for Women's Health Circle of Giving (JAS, USS), R01 AG079356 (JAS); NIH RF1 AG054199 (TI), R01 AG066429 (TI), R01 AG067763 (TI); Fundação para a Ciência e a Tecnologia, I.P., through iNOVA4Health (UIDB/04462/2020, UIDP/04462/2020) and the LS4FUTURE Associated Laboratory (LA/P/0087/2020) (JC). Funding Information: The authors are grateful to the respondents of the ISEV CSF Task Force survey, the time and efforts of the Task Force team members, and the members who provided feedback and edits for the final manuscript. This work was funded by NIH RF1 AG059392 (JAS), OHSU Center for Women's Health Circle of Giving (JAS, USS), R01 AG079356 (JAS); NIH RF1 AG054199 (TI), R01 AG066429 (TI), R01 AG067763 (TI); Fundação para a Ciência e a Tecnologia, I.P., through iNOVA4Health (UIDB/04462/2020, UIDP/04462/2020) and the LS4FUTURE Associated Laboratory (LA/P/0087/2020) (JC). Publisher Copyright: © 2023 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.
publishDate 2024
dc.date.none.fl_str_mv 2024-01
2024-01-01T00:00:00Z
2025-05-07T21:16:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/182730
url http://hdl.handle.net/10362/182730
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2001-3078
PURE: 83669663
https://doi.org/10.1002/jev2.12397
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833602925013762048

Registros relacionados