Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase

Bibliographic Details
Main Author: Domingues, Lucília
Publication Date: 2000
Other Authors: Onnela, M., Teixeira, J. A., Lima, Nelson, Penttilä, M.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/1772
Summary: One way of improving heterologous protein production is to use high cell density systems, one of the most attractive being the flocculating yeast production system. Also, lactose is available in large amounts as a waste product from cheese production processes. The construction of flocculent and non-flocculent brewer's yeast strains secreting β-galactosidase and growing on lactose is presented. A plasmid was constructed coding for an extracellular β-galactosidase of Aspergillus niger and having, as selective marker, the yeast CUP1 gene conferring resistance to copper. This selective marker allows for the transformation of wild-type yeasts. This work represents an important step towards the study of heterologous protein secretion by flocculent cells.
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spelling Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidaseScience & TechnologyOne way of improving heterologous protein production is to use high cell density systems, one of the most attractive being the flocculating yeast production system. Also, lactose is available in large amounts as a waste product from cheese production processes. The construction of flocculent and non-flocculent brewer's yeast strains secreting β-galactosidase and growing on lactose is presented. A plasmid was constructed coding for an extracellular β-galactosidase of Aspergillus niger and having, as selective marker, the yeast CUP1 gene conferring resistance to copper. This selective marker allows for the transformation of wild-type yeasts. This work represents an important step towards the study of heterologous protein secretion by flocculent cells.Fundação para a Ciência e a Tecnologia (FCT) – PRAXIS XXI/BD/11306/97.Finland. Center For International Mobility.European Science Foundation.Springer VerlagUniversidade do MinhoDomingues, LucíliaOnnela, M.Teixeira, J. A.Lima, NelsonPenttilä, M.20002000-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1772eng“Applied microbiology and biotechnology”. ISSN 0175-7598. 54 (2000) 97-103.0175-759810.1007/s00253000035810952011info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T06:25:48Zoai:repositorium.sdum.uminho.pt:1822/1772Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:52:59.374124Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
title Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
spellingShingle Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
Domingues, Lucília
Science & Technology
title_short Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
title_full Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
title_fullStr Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
title_full_unstemmed Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
title_sort Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
author Domingues, Lucília
author_facet Domingues, Lucília
Onnela, M.
Teixeira, J. A.
Lima, Nelson
Penttilä, M.
author_role author
author2 Onnela, M.
Teixeira, J. A.
Lima, Nelson
Penttilä, M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Domingues, Lucília
Onnela, M.
Teixeira, J. A.
Lima, Nelson
Penttilä, M.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description One way of improving heterologous protein production is to use high cell density systems, one of the most attractive being the flocculating yeast production system. Also, lactose is available in large amounts as a waste product from cheese production processes. The construction of flocculent and non-flocculent brewer's yeast strains secreting β-galactosidase and growing on lactose is presented. A plasmid was constructed coding for an extracellular β-galactosidase of Aspergillus niger and having, as selective marker, the yeast CUP1 gene conferring resistance to copper. This selective marker allows for the transformation of wild-type yeasts. This work represents an important step towards the study of heterologous protein secretion by flocculent cells.
publishDate 2000
dc.date.none.fl_str_mv 2000
2000-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1772
url http://hdl.handle.net/1822/1772
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv “Applied microbiology and biotechnology”. ISSN 0175-7598. 54 (2000) 97-103.
0175-7598
10.1007/s002530000358
10952011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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