The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes

Bibliographic Details
Main Author: Fonseca, Daniela
Publication Date: 2025
Other Authors: Martins, Nuno, Garcia, Raquel, Cabrita, Maria João
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10174/37637
https://doi.org/10.1016/j.lwt.2024.117314
Summary: This works focuses on developing an optimized methodology for analyzing free and bound volatile compounds in grapes using enzymatic hydrolysis with AR2000 enzyme and HS-SPME-GC × GC-ToFMS. To achieve this purpose, a previous optimization step of enzymatic hydrolysis was implemented and optimal conditions for enzymatic hydrolysis were established using 50 mg of AR2000 enzyme at 35 ◦C for 24 h, while the best extraction conditions were found to be 40 min at 60 ◦C. Applying this methodology to clones of Moreto grapes from harvest of 2022 and 2023, it was possible to identify and semi-quantify sixty volatile compounds, with more compounds identifiable in 2022 than in 2023. The results also showed the influence of crop year and genetic intravariability
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spelling The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapesAroma compositionHS-SPME-GC×GC-ToFMSAR2000 enzymeClonesThis works focuses on developing an optimized methodology for analyzing free and bound volatile compounds in grapes using enzymatic hydrolysis with AR2000 enzyme and HS-SPME-GC × GC-ToFMS. To achieve this purpose, a previous optimization step of enzymatic hydrolysis was implemented and optimal conditions for enzymatic hydrolysis were established using 50 mg of AR2000 enzyme at 35 ◦C for 24 h, while the best extraction conditions were found to be 40 min at 60 ◦C. Applying this methodology to clones of Moreto grapes from harvest of 2022 and 2023, it was possible to identify and semi-quantify sixty volatile compounds, with more compounds identifiable in 2022 than in 2023. The results also showed the influence of crop year and genetic intravariabilityElsevier - LWT2025-01-07T16:02:38Z2025-01-072025-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/37637http://hdl.handle.net/10174/37637https://doi.org/10.1016/j.lwt.2024.117314engndnmartins@uevora.ptraquelg@uevora.ptmjbc@uevora.pt210Fonseca, DanielaMartins, NunoGarcia, RaquelCabrita, Maria Joãoinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-01-14T01:46:58Zoai:dspace.uevora.pt:10174/37637Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T19:39:21.719904Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes
title The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes
spellingShingle The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes
Fonseca, Daniela
Aroma composition
HS-SPME-GC×GC-ToFMS
AR2000 enzyme
Clones
title_short The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes
title_full The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes
title_fullStr The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes
title_full_unstemmed The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes
title_sort The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes
author Fonseca, Daniela
author_facet Fonseca, Daniela
Martins, Nuno
Garcia, Raquel
Cabrita, Maria João
author_role author
author2 Martins, Nuno
Garcia, Raquel
Cabrita, Maria João
author2_role author
author
author
dc.contributor.author.fl_str_mv Fonseca, Daniela
Martins, Nuno
Garcia, Raquel
Cabrita, Maria João
dc.subject.por.fl_str_mv Aroma composition
HS-SPME-GC×GC-ToFMS
AR2000 enzyme
Clones
topic Aroma composition
HS-SPME-GC×GC-ToFMS
AR2000 enzyme
Clones
description This works focuses on developing an optimized methodology for analyzing free and bound volatile compounds in grapes using enzymatic hydrolysis with AR2000 enzyme and HS-SPME-GC × GC-ToFMS. To achieve this purpose, a previous optimization step of enzymatic hydrolysis was implemented and optimal conditions for enzymatic hydrolysis were established using 50 mg of AR2000 enzyme at 35 ◦C for 24 h, while the best extraction conditions were found to be 40 min at 60 ◦C. Applying this methodology to clones of Moreto grapes from harvest of 2022 and 2023, it was possible to identify and semi-quantify sixty volatile compounds, with more compounds identifiable in 2022 than in 2023. The results also showed the influence of crop year and genetic intravariability
publishDate 2025
dc.date.none.fl_str_mv 2025-01-07T16:02:38Z
2025-01-07
2025-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/37637
http://hdl.handle.net/10174/37637
https://doi.org/10.1016/j.lwt.2024.117314
url http://hdl.handle.net/10174/37637
https://doi.org/10.1016/j.lwt.2024.117314
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv nd
nmartins@uevora.pt
raquelg@uevora.pt
mjbc@uevora.pt
210
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier - LWT
publisher.none.fl_str_mv Elsevier - LWT
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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