The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium

Bibliographic Details
Main Author: Amaral, Ana
Publication Date: 2021
Other Authors: Fernandes, Carina, Szostek-Mioduchowska, Anna, Lukasik, Karolina, Rebordão, Maria Rosa, Pinto-Bravo, Pedro, Skarzynski, Dariusz Jan, Ferreira-Dias, Graça
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.5/23107
Summary: Research Areas: Life Sciences & Biomedicine ; Microbiology
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spelling The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometriumEquineEndometrosisFibrosisCollagenCathepsin GNoscapineInhibitionResearch Areas: Life Sciences & Biomedicine ; MicrobiologyABSTRACT - Cathepsin G (CAT) is a protease released by neutrophils when forming neutrophil extracellular traps that was already associated with inducing type I collagen (COL1) in equine endometrium in vitro. Endometrosis is a fibrotic condition mainly characterized by COL1 deposition in the equine endometrium. The objective was to evaluate if noscapine (an alkaloid for cough treatment with anti-neoplastic and anti-fibrotic properties) would reduce COL1A2 transcription (evaluated by qPCR) and COL1 protein relative abundance (evaluated by western blot) induced by CAT in equine endometrial explants from follicular and mid-luteal phases treated for 24 or 48 h. The explants treated with CAT increased COL1 expression. Noscapine decreased COL1A2 transcription at both estrous cycle phases, but COL1 relative protein only at the follicular phase, both induced by CAT. Additionally, the noscapine anti-fibrotic action was found to be more effective in the follicular phase. The CAT treatment caused more fibrosis at the longest period of treatment, while noscapine acted better at the shortest time of treatment. Our results showed that noscapine could act as an anti-fibrotic drug in equine endometrosis by inhibiting CAT in vitro. Noscapine offers a new promising therapeutic tool for treating fibrosis as a single non-selective agent to be considered in the future.MDPIRepositório da Universidade de LisboaAmaral, AnaFernandes, CarinaSzostek-Mioduchowska, AnnaLukasik, KarolinaRebordão, Maria RosaPinto-Bravo, PedroSkarzynski, Dariusz JanFerreira-Dias, Graça2022-01-17T14:52:03Z2021-10-192021-10-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23107engAmaral A, Fernandes C, Szostek-Mioduchowska A, Lukasik K, Rebordao MR) 1, 3 Pinto-Bravo, P (Pinto-Bravo, Pedro) 3 Skarzynski, DJ, Ferreira-Dias G. 2021. The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium. Life 11(10):1107. Doi: 10.3390/life1110110710.3390/life111011072075-1729info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T15:24:14Zoai:repositorio.ulisboa.pt:10400.5/23107Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:42:23.933190Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium
title The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium
spellingShingle The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium
Amaral, Ana
Equine
Endometrosis
Fibrosis
Collagen
Cathepsin G
Noscapine
Inhibition
title_short The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium
title_full The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium
title_fullStr The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium
title_full_unstemmed The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium
title_sort The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium
author Amaral, Ana
author_facet Amaral, Ana
Fernandes, Carina
Szostek-Mioduchowska, Anna
Lukasik, Karolina
Rebordão, Maria Rosa
Pinto-Bravo, Pedro
Skarzynski, Dariusz Jan
Ferreira-Dias, Graça
author_role author
author2 Fernandes, Carina
Szostek-Mioduchowska, Anna
Lukasik, Karolina
Rebordão, Maria Rosa
Pinto-Bravo, Pedro
Skarzynski, Dariusz Jan
Ferreira-Dias, Graça
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Amaral, Ana
Fernandes, Carina
Szostek-Mioduchowska, Anna
Lukasik, Karolina
Rebordão, Maria Rosa
Pinto-Bravo, Pedro
Skarzynski, Dariusz Jan
Ferreira-Dias, Graça
dc.subject.por.fl_str_mv Equine
Endometrosis
Fibrosis
Collagen
Cathepsin G
Noscapine
Inhibition
topic Equine
Endometrosis
Fibrosis
Collagen
Cathepsin G
Noscapine
Inhibition
description Research Areas: Life Sciences & Biomedicine ; Microbiology
publishDate 2021
dc.date.none.fl_str_mv 2021-10-19
2021-10-19T00:00:00Z
2022-01-17T14:52:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23107
url http://hdl.handle.net/10400.5/23107
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Amaral A, Fernandes C, Szostek-Mioduchowska A, Lukasik K, Rebordao MR) 1, 3 Pinto-Bravo, P (Pinto-Bravo, Pedro) 3 Skarzynski, DJ, Ferreira-Dias G. 2021. The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium. Life 11(10):1107. Doi: 10.3390/life11101107
10.3390/life11101107
2075-1729
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833601801976283136

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