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Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern Mozambique

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Main Author: Manjate, Filomena
Publication Date: 2024
Other Authors: João, Eva D., Mwangi, Peter, Chirinda, Percina, Mogotsi, Milton, Garrine, Marcelino, Messa, Augusto, Vubil, Delfino, Nobela, Nélio, Kotloff, Karen, Nataro, James P., Nhampossa, Tacilta, Acácio, Sozinho, Weldegebriel, Goitom, Tate, Jacqueline E., Parashar, Umesh, Mwenda, Jason M., Alonso, Pedro L., Cunha, Celso, Nyaga, Martin, Mandomando, Inácio
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/178500
Summary: 0.870\u2009\u2212\u200915 SC; The United States Agency for International Development (USAID), grant number AID-656-F-16-00002 and Fundo Nacional de Investiga\u00E7\u00E3o (FNI), Mo\u00E7ambique, grant number 245-INV funded the implementation of the surveillance of rotavirus and other enteropathogens in children less than 5 years of age in Manhi\u00E7a. The whole genome characterization undertaken in this study was supported by The Child Health and Mortality Prevention program (Surveillance), CHAMPS funded by the Bill & Melinda Gates Foundation under Grant OPP1126780; The Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia for funds to GHTM-UID/04413/2020 and LA-REAL \u2013 LA/P/0117/2020, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Portugal and the Next Generation Sequencing Unit, and the Division of Virology, Faculty of Health Sciences, University of the Free State, South Africa. The Calouste Gulbenkian Foundation financed F. M\u2019s PhD studies, under the grant number 234066. CISM receives core funding from the Mozambican government and the \u201CAgencia Espa\u00F1ola de Cooperacion Internacional para el Desarollo (AECID).\u201D Publisher Copyright: © The Author(s) 2024.
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spelling Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern MozambiqueChildren with diarrhoeaChildren without diarrhoeaManhiça districtMozambiqueRotavirus group AWhole genome sequencingGeneralSDG 3 - Good Health and Well-being0.870\u2009\u2212\u200915 SC; The United States Agency for International Development (USAID), grant number AID-656-F-16-00002 and Fundo Nacional de Investiga\u00E7\u00E3o (FNI), Mo\u00E7ambique, grant number 245-INV funded the implementation of the surveillance of rotavirus and other enteropathogens in children less than 5 years of age in Manhi\u00E7a. The whole genome characterization undertaken in this study was supported by The Child Health and Mortality Prevention program (Surveillance), CHAMPS funded by the Bill & Melinda Gates Foundation under Grant OPP1126780; The Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia for funds to GHTM-UID/04413/2020 and LA-REAL \u2013 LA/P/0117/2020, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Portugal and the Next Generation Sequencing Unit, and the Division of Virology, Faculty of Health Sciences, University of the Free State, South Africa. The Calouste Gulbenkian Foundation financed F. M\u2019s PhD studies, under the grant number 234066. CISM receives core funding from the Mozambican government and the \u201CAgencia Espa\u00F1ola de Cooperacion Internacional para el Desarollo (AECID).\u201D Publisher Copyright: © The Author(s) 2024.Post rotavirus vaccine introduction in Mozambique (September 2015), we documented a decline in rotavirus-associated diarrhoea and genotypes changes in our diarrhoeal surveillance spanning 2008–2021. This study aimed to perform whole-genome sequencing of rotavirus strains from 2009 to 2012 (pre-vaccine) and 2017–2018 (post-vaccine). Rotavirus strains previously detected by conventional PCR as G2P[4], G2P[6], G3P[4], G8P[4], G8P[6], and G9P[6] from children with moderate-to-severe and less-severe diarrhoea and without diarrhoea (healthy community controls) were sequenced using Illumina MiSeq® platform and analysed using bioinformatics tools. All these G and P-type combinations exhibited DS-1-like constellation in the rest of the genome segments as, I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that strains from children with and without diarrhoea clustered together with other Mozambican and global strains. Notably, the NSP4 gene of strains G3P[4] and G8P[4] in children with diarrhoea clustered with animal strains, such as bovine and caprine, with similarity identities ranging from 89.1 to 97.0% nucleotide and 89.5-97.0% amino acids. Our findings revealed genetic similarities among rotavirus strains from children with and without diarrhoea, as well as with animal strains, reinforcing the need of implementing studies with One Health approach in our setting, to elucidate the genetic diversity of this important pathogen.TB, HIV and opportunistic diseases and pathogens (THOP)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo IHMT)RUNManjate, FilomenaJoão, Eva D.Mwangi, PeterChirinda, PercinaMogotsi, MiltonGarrine, MarcelinoMessa, AugustoVubil, DelfinoNobela, NélioKotloff, KarenNataro, James P.Nhampossa, TaciltaAcácio, SozinhoWeldegebriel, GoitomTate, Jacqueline E.Parashar, UmeshMwenda, Jason M.Alonso, Pedro L.Cunha, CelsoNyaga, MartinMandomando, Inácio2025-02-05T21:20:05Z2024-12-282024-12-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/10362/178500eng2045-2322PURE: 108511128https://doi.org/10.1038/s41598-024-79767-4info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-10T01:39:30Zoai:run.unl.pt:10362/178500Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T19:46:51.672171Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern Mozambique
title Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern Mozambique
spellingShingle Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern Mozambique
Manjate, Filomena
Children with diarrhoea
Children without diarrhoea
Manhiça district
Mozambique
Rotavirus group A
Whole genome sequencing
General
SDG 3 - Good Health and Well-being
title_short Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern Mozambique
title_full Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern Mozambique
title_fullStr Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern Mozambique
title_full_unstemmed Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern Mozambique
title_sort Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern Mozambique
author Manjate, Filomena
author_facet Manjate, Filomena
João, Eva D.
Mwangi, Peter
Chirinda, Percina
Mogotsi, Milton
Garrine, Marcelino
Messa, Augusto
Vubil, Delfino
Nobela, Nélio
Kotloff, Karen
Nataro, James P.
Nhampossa, Tacilta
Acácio, Sozinho
Weldegebriel, Goitom
Tate, Jacqueline E.
Parashar, Umesh
Mwenda, Jason M.
Alonso, Pedro L.
Cunha, Celso
Nyaga, Martin
Mandomando, Inácio
author_role author
author2 João, Eva D.
Mwangi, Peter
Chirinda, Percina
Mogotsi, Milton
Garrine, Marcelino
Messa, Augusto
Vubil, Delfino
Nobela, Nélio
Kotloff, Karen
Nataro, James P.
Nhampossa, Tacilta
Acácio, Sozinho
Weldegebriel, Goitom
Tate, Jacqueline E.
Parashar, Umesh
Mwenda, Jason M.
Alonso, Pedro L.
Cunha, Celso
Nyaga, Martin
Mandomando, Inácio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv TB, HIV and opportunistic diseases and pathogens (THOP)
Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo IHMT)
RUN
dc.contributor.author.fl_str_mv Manjate, Filomena
João, Eva D.
Mwangi, Peter
Chirinda, Percina
Mogotsi, Milton
Garrine, Marcelino
Messa, Augusto
Vubil, Delfino
Nobela, Nélio
Kotloff, Karen
Nataro, James P.
Nhampossa, Tacilta
Acácio, Sozinho
Weldegebriel, Goitom
Tate, Jacqueline E.
Parashar, Umesh
Mwenda, Jason M.
Alonso, Pedro L.
Cunha, Celso
Nyaga, Martin
Mandomando, Inácio
dc.subject.por.fl_str_mv Children with diarrhoea
Children without diarrhoea
Manhiça district
Mozambique
Rotavirus group A
Whole genome sequencing
General
SDG 3 - Good Health and Well-being
topic Children with diarrhoea
Children without diarrhoea
Manhiça district
Mozambique
Rotavirus group A
Whole genome sequencing
General
SDG 3 - Good Health and Well-being
description 0.870\u2009\u2212\u200915 SC; The United States Agency for International Development (USAID), grant number AID-656-F-16-00002 and Fundo Nacional de Investiga\u00E7\u00E3o (FNI), Mo\u00E7ambique, grant number 245-INV funded the implementation of the surveillance of rotavirus and other enteropathogens in children less than 5 years of age in Manhi\u00E7a. The whole genome characterization undertaken in this study was supported by The Child Health and Mortality Prevention program (Surveillance), CHAMPS funded by the Bill & Melinda Gates Foundation under Grant OPP1126780; The Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia for funds to GHTM-UID/04413/2020 and LA-REAL \u2013 LA/P/0117/2020, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Portugal and the Next Generation Sequencing Unit, and the Division of Virology, Faculty of Health Sciences, University of the Free State, South Africa. The Calouste Gulbenkian Foundation financed F. M\u2019s PhD studies, under the grant number 234066. CISM receives core funding from the Mozambican government and the \u201CAgencia Espa\u00F1ola de Cooperacion Internacional para el Desarollo (AECID).\u201D Publisher Copyright: © The Author(s) 2024.
publishDate 2024
dc.date.none.fl_str_mv 2024-12-28
2024-12-28T00:00:00Z
2025-02-05T21:20:05Z
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