Streptococcus pneumoniae carriage, serotypes, genotypes, and antimicrobial resistance trends among children in Portugal, after introduction of PCV13 in National Immunization Program
| Main Author: | |
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| Publication Date: | 2024 |
| Other Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | http://hdl.handle.net/10362/182747 |
Summary: | Funding Information: This work was supported by unrestricted research grant from Pfizer (to RSL), and through MOSTMICRO (LISBOA-01-0145-FEDER-007660) funded by FEDER through COMPETE2020 \u2013 Programa Operacional Competitividade e Internacionaliza\u00E7\u00E3o (POCI) . CC and BF were supported by grants PD/BD/148434/2019 and 2020.05293.BD, respectively. Publisher Copyright: © 2024 The Authors |
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Streptococcus pneumoniae carriage, serotypes, genotypes, and antimicrobial resistance trends among children in Portugal, after introduction of PCV13 in National Immunization ProgramA cross-sectional studyAntimicrobial resistanceCarriageGPSCPneumococcal conjugate vaccineSerotypeStreptococcus pneumoniaeMolecular MedicineImmunology and Microbiology(all)veterinary(all)Public Health, Environmental and Occupational HealthInfectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: This work was supported by unrestricted research grant from Pfizer (to RSL), and through MOSTMICRO (LISBOA-01-0145-FEDER-007660) funded by FEDER through COMPETE2020 \u2013 Programa Operacional Competitividade e Internacionaliza\u00E7\u00E3o (POCI) . CC and BF were supported by grants PD/BD/148434/2019 and 2020.05293.BD, respectively. Publisher Copyright: © 2024 The AuthorsStreptococcus pneumoniae carriage studies are crucial to monitor changes induced by use of pneumococcal conjugate vaccines and inform vaccination policies. In this cross-sectional study, we examined changes within the pneumococcal population following introduction of PCV13 in 2015 in the National Immunization Program (NIP), in Portugal. In 2018–2020 (NIP-PCV13), we obtained 1450 nasopharyngeal samples from children ≤6 years attending day-care. We assessed serotypes, antimicrobial resistance, and genotypes (MLST and GPSC) and compared findings with earlier periods: 2009–2010 (pre-PCV13), 2011–2012 (early-PCV13), and 2015–2016 (late-PCV13). Pneumococcal carriage prevalence remained stable at 60.2 %. Carriage of PCV13 serotypes was 10.7 %, markedly reduced compared to pre-PCV13 period (47.6 %). The most prevalent PCV13 serotypes were 19F, 3, and 19A all showing a significant decreasing trend compared to the pre-PCV13 period (from 7.1 % to 4.7 %, 10.1 % to 1.8 %, and 14.1 % to 1.8 %, respectively), a notable observation given the described limited effectiveness of PCV13 against serotype 3. Non-vaccinated children and children aged 4–6 years were more likely to carry PCV13 serotypes (2.5-fold, 95 %CI [1.1–5.6], and 2.9-fold, 95 %CI [1.3–6.8], respectively). The most prevalent non-PCV13 serotypes were 15B/C, 11A, 23B, 23A, and NT, collectively accounting for 51.9 % of all isolates. In total, 30.5 % of all pneumococci were potentially covered by PCV20. Resistance to penicillin (low-level) and macrolides increased significantly, from 9.3 % and 13.4 %, respectively, in the late-PCV13 period, to approximately 20 % each, mostly due to lineages expressing non-PCV13 serotypes, nearing pre-PCV13 levels. An expansion of lineages traditionally associated with PCV13 serotypes, like CC156-GPSC6 (serotype 14) and CC193-GPSC11 (serotype 19F), but now predominantly expressing non-PCV13 serotypes (11A, 15B/C, and 24F for GPSC6; and 15A and 21 for GPSC11) was noted. These findings indicate that the pneumococcal population is adapting to the pressures conferred by PCV13 and antimicrobial use and indicate the need to maintain close surveillance.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)Molecular, Structural and Cellular Microbiology (MOSTMICRO)RUNCandeias, CatarinaAlmeida, Sónia T.Paulo, A. CristinaSimões, Alexandra S.Ferreira, BárbaraCruz, Ana R.Queirós, MiguelTouret, TiagoBrito-Avô, Antóniode Lencastre, HermíniaSá-Leão, Raquel2025-05-07T21:19:27Z2024-09-172024-09-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/182747eng0264-410XPURE: 99909567https://doi.org/10.1016/j.vaccine.2024.126219info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-05-19T01:39:22Zoai:run.unl.pt:10362/182747Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T07:13:46.780359Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Streptococcus pneumoniae carriage, serotypes, genotypes, and antimicrobial resistance trends among children in Portugal, after introduction of PCV13 in National Immunization Program A cross-sectional study |
| title |
Streptococcus pneumoniae carriage, serotypes, genotypes, and antimicrobial resistance trends among children in Portugal, after introduction of PCV13 in National Immunization Program |
| spellingShingle |
Streptococcus pneumoniae carriage, serotypes, genotypes, and antimicrobial resistance trends among children in Portugal, after introduction of PCV13 in National Immunization Program Candeias, Catarina Antimicrobial resistance Carriage GPSC Pneumococcal conjugate vaccine Serotype Streptococcus pneumoniae Molecular Medicine Immunology and Microbiology(all) veterinary(all) Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| title_short |
Streptococcus pneumoniae carriage, serotypes, genotypes, and antimicrobial resistance trends among children in Portugal, after introduction of PCV13 in National Immunization Program |
| title_full |
Streptococcus pneumoniae carriage, serotypes, genotypes, and antimicrobial resistance trends among children in Portugal, after introduction of PCV13 in National Immunization Program |
| title_fullStr |
Streptococcus pneumoniae carriage, serotypes, genotypes, and antimicrobial resistance trends among children in Portugal, after introduction of PCV13 in National Immunization Program |
| title_full_unstemmed |
Streptococcus pneumoniae carriage, serotypes, genotypes, and antimicrobial resistance trends among children in Portugal, after introduction of PCV13 in National Immunization Program |
| title_sort |
Streptococcus pneumoniae carriage, serotypes, genotypes, and antimicrobial resistance trends among children in Portugal, after introduction of PCV13 in National Immunization Program |
| author |
Candeias, Catarina |
| author_facet |
Candeias, Catarina Almeida, Sónia T. Paulo, A. Cristina Simões, Alexandra S. Ferreira, Bárbara Cruz, Ana R. Queirós, Miguel Touret, Tiago Brito-Avô, António de Lencastre, Hermínia Sá-Leão, Raquel |
| author_role |
author |
| author2 |
Almeida, Sónia T. Paulo, A. Cristina Simões, Alexandra S. Ferreira, Bárbara Cruz, Ana R. Queirós, Miguel Touret, Tiago Brito-Avô, António de Lencastre, Hermínia Sá-Leão, Raquel |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Tecnologia Química e Biológica António Xavier (ITQB) Molecular, Structural and Cellular Microbiology (MOSTMICRO) RUN |
| dc.contributor.author.fl_str_mv |
Candeias, Catarina Almeida, Sónia T. Paulo, A. Cristina Simões, Alexandra S. Ferreira, Bárbara Cruz, Ana R. Queirós, Miguel Touret, Tiago Brito-Avô, António de Lencastre, Hermínia Sá-Leão, Raquel |
| dc.subject.por.fl_str_mv |
Antimicrobial resistance Carriage GPSC Pneumococcal conjugate vaccine Serotype Streptococcus pneumoniae Molecular Medicine Immunology and Microbiology(all) veterinary(all) Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| topic |
Antimicrobial resistance Carriage GPSC Pneumococcal conjugate vaccine Serotype Streptococcus pneumoniae Molecular Medicine Immunology and Microbiology(all) veterinary(all) Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| description |
Funding Information: This work was supported by unrestricted research grant from Pfizer (to RSL), and through MOSTMICRO (LISBOA-01-0145-FEDER-007660) funded by FEDER through COMPETE2020 \u2013 Programa Operacional Competitividade e Internacionaliza\u00E7\u00E3o (POCI) . CC and BF were supported by grants PD/BD/148434/2019 and 2020.05293.BD, respectively. Publisher Copyright: © 2024 The Authors |
| publishDate |
2024 |
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2024-09-17 2024-09-17T00:00:00Z 2025-05-07T21:19:27Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://hdl.handle.net/10362/182747 |
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http://hdl.handle.net/10362/182747 |
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eng |
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eng |
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0264-410X PURE: 99909567 https://doi.org/10.1016/j.vaccine.2024.126219 |
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