Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi

Bibliographic Details
Main Author: Serrazina, Susana
Publication Date: 2021
Other Authors: Machado, Helena, Costa, Rita Lourenço, Duque, Paula, Malhó, Rui
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.5/21375
Summary: Original Research
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spelling Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomiArabidopsis thalianaallene oxide synthaseplant defensegene functional analysisPhytophthora cinnamomiCastanea crenataCastanea sativaOriginal ResearchAllene oxide synthase (AOS) is a key enzyme of the jasmonic acid (JA) signaling pathway. The AOS gene was previously found to be upregulated in an Asian chestnut species resistant to infection by the oomycete Phytophthora cinnamomi (Castanea crenata), while lower expression values were detected in the susceptible European chestnut (Castanea sativa). Here, we report a genetic and functional characterization of the C. crenata AOS (CcAOS) upon its heterologous gene expression in a susceptible ecotype of Arabidopsis thaliana, which contains a single AOS gene. It was found that Arabidopsis plants expressing CcAOS delay pathogen progression and exhibit more vigorous growth in its presence. They also show upregulation of jasmonic acid and salicylic acid-related genes. As in its native species, heterologous CcAOS localized to plastids, as revealed by confocal imaging of the CcAOS-eGFP fusion protein in transgenic Arabidopsis roots. This observation was confirmed upon transient expression in Nicotiana benthamiana leaf epidermal cells. To further confirm a specific role of CcAOS in the defense mechanism against the pathogen, we performed crosses between transgenic CcAOS plants and an infertile Arabidopsis AOS knockout mutant line. It was found that plants expressing CcAOS exhibit normal growth, remain infertile but are significantly more tolerant to the pathogen than wild type plants. Together, our results indicate that CcAOS is an important player in plant defense responses against oomycete infection and that its expression in susceptible varieties may be a valuable tool to mitigate biotic stress responsesFrontiers in Plant ScienceRepositório da Universidade de LisboaSerrazina, SusanaMachado, HelenaCosta, Rita LourençoDuque, PaulaMalhó, Rui2021-05-28T10:11:34Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/21375engSerrazina S, Machado H, Costa RL, Duque P and Malhó R (2021) Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi. Front. Plant Sci. 12:62869710.3389/fpls.2021.628697info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T15:58:57Zoai:repositorio.ulisboa.pt:10400.5/21375Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:59:16.139548Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi
title Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi
spellingShingle Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi
Serrazina, Susana
Arabidopsis thaliana
allene oxide synthase
plant defense
gene functional analysis
Phytophthora cinnamomi
Castanea crenata
Castanea sativa
title_short Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi
title_full Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi
title_fullStr Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi
title_full_unstemmed Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi
title_sort Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi
author Serrazina, Susana
author_facet Serrazina, Susana
Machado, Helena
Costa, Rita Lourenço
Duque, Paula
Malhó, Rui
author_role author
author2 Machado, Helena
Costa, Rita Lourenço
Duque, Paula
Malhó, Rui
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Serrazina, Susana
Machado, Helena
Costa, Rita Lourenço
Duque, Paula
Malhó, Rui
dc.subject.por.fl_str_mv Arabidopsis thaliana
allene oxide synthase
plant defense
gene functional analysis
Phytophthora cinnamomi
Castanea crenata
Castanea sativa
topic Arabidopsis thaliana
allene oxide synthase
plant defense
gene functional analysis
Phytophthora cinnamomi
Castanea crenata
Castanea sativa
description Original Research
publishDate 2021
dc.date.none.fl_str_mv 2021-05-28T10:11:34Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/21375
url http://hdl.handle.net/10400.5/21375
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Serrazina S, Machado H, Costa RL, Duque P and Malhó R (2021) Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi. Front. Plant Sci. 12:628697
10.3389/fpls.2021.628697
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers in Plant Science
publisher.none.fl_str_mv Frontiers in Plant Science
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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