Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes

Bibliographic Details
Main Author: Rodrigues, Margarida
Publication Date: 2012
Other Authors: Santos, Andrea, de la Torre, Beatriz G., Rádis-Baptista, Gandhi, Andreu, David, Santos, Nuno C.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/10770
Summary: © 2012 Elsevier B.V. All rights reserved.
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spelling Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranesNucleolar-targeting peptideCrotamineCell-penetrating peptideMembrane partitionMembrane translocationFluorescence spectroscopy© 2012 Elsevier B.V. All rights reserved.A novel class of cell-penetrating, nucleolar-targeting peptides (NrTPs), was recently developed from the rattlesnake venom toxin crotamine. Based on the intrinsic fluorescence of tyrosine or tryptophan residues, the partition of NrTPs and crotamine to membranes with variable lipid compositions was studied. Partition coefficient values (in the 102–105 range) followed essentially the compositional trend POPC:POPG≤POPGbPOPC≤POPC: cholesterol. Leakage assays showed that NrTPs induce minimal lipid vesicle disruption. Fluorescence quenching of NrTPs, either by acrylamide or lipophilic probes, revealed that NrTPs are buried in the lipid bilayer only for negatively-charged membranes. Adoption of partial secondary structure by the NrTPs upon interaction with POPC and POPG vesicles was demonstrated by circular dichroism. Translocation studies were conducted using a novel methodology, based on the confocal microscopy imaging of giant multilamellar vesicles or giant multivesicular liposomes.With this new procedure, which can now be used to evaluate the membrane translocation ability of other molecules, it was demonstrated that NrTPs are able to cross lipidmembranes even in the absence of a receptor or transmembrane gradient. Altogether, these results indicate that NrTPs interactwith lipid bilayers and can penetrate cells via different entry mechanisms, reinforcing the applicability of this class of peptide as therapeutic tools for the delivery of molecular cargoes.This work was funded by the Portuguese Ministry of Education and Science (Fundação para a Ciência e a Tecnologia, FCT-MEC; including M.R. and A.S. fellowships SFRH/BD/37432/2007 and SFRH/BPD/26821/2006, respectively), the Spanish Ministry of Economy and Competitiveness (MINECO, grant SAF2011-24899), Generalitat de Catalunya (2009 SGR 492), FP7-PEOPLE IRSES (International Research Staff Exchange Scheme) project MEMPEPACROSS (European Union), and the European Biophysical Societies’ Association (EBSA).ElsevierRepositório da Universidade de LisboaRodrigues, MargaridaSantos, Andreade la Torre, Beatriz G.Rádis-Baptista, GandhiAndreu, DavidSantos, Nuno C.2014-03-21T14:48:37Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10770engBiochimica et Biophysica Acta 1818 (2012) 2707–27170006-3002http://dx.doi.org/10.1016/j.bbamem.2012.06.014metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:06:52Zoai:repositorio.ulisboa.pt:10451/10770Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:35:11.567438Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes
title Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes
spellingShingle Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes
Rodrigues, Margarida
Nucleolar-targeting peptide
Crotamine
Cell-penetrating peptide
Membrane partition
Membrane translocation
Fluorescence spectroscopy
title_short Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes
title_full Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes
title_fullStr Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes
title_full_unstemmed Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes
title_sort Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes
author Rodrigues, Margarida
author_facet Rodrigues, Margarida
Santos, Andrea
de la Torre, Beatriz G.
Rádis-Baptista, Gandhi
Andreu, David
Santos, Nuno C.
author_role author
author2 Santos, Andrea
de la Torre, Beatriz G.
Rádis-Baptista, Gandhi
Andreu, David
Santos, Nuno C.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Rodrigues, Margarida
Santos, Andrea
de la Torre, Beatriz G.
Rádis-Baptista, Gandhi
Andreu, David
Santos, Nuno C.
dc.subject.por.fl_str_mv Nucleolar-targeting peptide
Crotamine
Cell-penetrating peptide
Membrane partition
Membrane translocation
Fluorescence spectroscopy
topic Nucleolar-targeting peptide
Crotamine
Cell-penetrating peptide
Membrane partition
Membrane translocation
Fluorescence spectroscopy
description © 2012 Elsevier B.V. All rights reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-03-21T14:48:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10770
url http://hdl.handle.net/10451/10770
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochimica et Biophysica Acta 1818 (2012) 2707–2717
0006-3002
http://dx.doi.org/10.1016/j.bbamem.2012.06.014
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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