Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain

Bibliographic Details
Main Author: Santos, Susana Isabel Duarte dos
Publication Date: 2019
Format: Master thesis
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/130114
Summary: "Staphylococcus epidermidis is a Gram-positive commensal bacterium found mainly in mucous membranes but also in sebaceous areas. It is considered an opportunistic pathogen associated with nosocomial infections. Indeed, S. epidermidis is the main cause of coagulase negative staphylococci infections associated with medical devices. This thesis aims to understand the impact of pH conditions which mimic the skin pH (5.5) and blood pH (7.4) in a S. epidermidis commensal strain (19N) biological processes and metabolic pathways. Proteomic studies were realized by nanoscale liquid chromatography-tandem mass spectrometry (nano LC-MS/MS) and metabolomic studies through nuclear magnetic resonance (NMR) in the whole cellular extract."
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spelling Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strainStaphylococcus epidermidisinfection processproteomicmetabolomic"Staphylococcus epidermidis is a Gram-positive commensal bacterium found mainly in mucous membranes but also in sebaceous areas. It is considered an opportunistic pathogen associated with nosocomial infections. Indeed, S. epidermidis is the main cause of coagulase negative staphylococci infections associated with medical devices. This thesis aims to understand the impact of pH conditions which mimic the skin pH (5.5) and blood pH (7.4) in a S. epidermidis commensal strain (19N) biological processes and metabolic pathways. Proteomic studies were realized by nanoscale liquid chromatography-tandem mass spectrometry (nano LC-MS/MS) and metabolomic studies through nuclear magnetic resonance (NMR) in the whole cellular extract."Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António XavierRUNSantos, Susana Isabel Duarte dos2022-11-30T01:34:03Z2019-11-292019-11-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/130114enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:57:49Zoai:run.unl.pt:10362/130114Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:29:05.718644Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
title Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
spellingShingle Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
Santos, Susana Isabel Duarte dos
Staphylococcus epidermidis
infection process
proteomic
metabolomic
title_short Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
title_full Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
title_fullStr Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
title_full_unstemmed Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
title_sort Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
author Santos, Susana Isabel Duarte dos
author_facet Santos, Susana Isabel Duarte dos
author_role author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Santos, Susana Isabel Duarte dos
dc.subject.por.fl_str_mv Staphylococcus epidermidis
infection process
proteomic
metabolomic
topic Staphylococcus epidermidis
infection process
proteomic
metabolomic
description "Staphylococcus epidermidis is a Gram-positive commensal bacterium found mainly in mucous membranes but also in sebaceous areas. It is considered an opportunistic pathogen associated with nosocomial infections. Indeed, S. epidermidis is the main cause of coagulase negative staphylococci infections associated with medical devices. This thesis aims to understand the impact of pH conditions which mimic the skin pH (5.5) and blood pH (7.4) in a S. epidermidis commensal strain (19N) biological processes and metabolic pathways. Proteomic studies were realized by nanoscale liquid chromatography-tandem mass spectrometry (nano LC-MS/MS) and metabolomic studies through nuclear magnetic resonance (NMR) in the whole cellular extract."
publishDate 2019
dc.date.none.fl_str_mv 2019-11-29
2019-11-29T00:00:00Z
2022-11-30T01:34:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/130114
url http://hdl.handle.net/10362/130114
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier
publisher.none.fl_str_mv Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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