Functional aspects of hybrid genome interaction : sex determination and gene expression regulation in the Squalius alburnoides complex

Bibliographic Details
Main Author: Pala, Irene Alexandra de Carvalho Braz dos Santos, 1981-
Publication Date: 2008
Language: por
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/1594
Summary: Tese de doutoramento em Biologia (Biologia Evolutiva) apresentada à Universidade de Lisboa através da Faculdade de Ciências, 2009.
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spelling Functional aspects of hybrid genome interaction : sex determination and gene expression regulation in the Squalius alburnoides complexHibridaçãoPoliploidiaDeterminação do sexoExpressão genéticaBiologia evolutivaTeses de doutoramento - 2009Tese de doutoramento em Biologia (Biologia Evolutiva) apresentada à Universidade de Lisboa através da Faculdade de Ciências, 2009.Hybrid polyploidy success has been extensively reported in plant species and more restrictedly in animals, but the regulatory changes that contribute to genome stabilization and regulation in the presence of distinct chromosome sets are still elusive. Due to its unique features among polyploidy taxa, the Squalius alburnoides complex of hybrid fish is a very desirable system to address these questions. It is considered an example of polyploid viability and evolutionary success, thus making it more interesting as a model to assess whether such features could be the result of an underlying specific gene regulatory basis. The aim of this dissertation was to assess whether hybridisation and polyploidy could have a functional effect over gene expression regulation: at the level of sex determination, a more strictly regulated process of gene interactions that apparently could be disrupted in the hybrids, as well as in a broader context regarding the contribution of each genome to the overall gene expression patterns. Six conserved elements of sex determination cascades (vasa, amh, dmrt1, wt1, dax1 e figla) were isolated, and their expression patterns and potential contributions were characterized in the adult gonads of S. alburnoides and the parental species S. pyrenaicus. Transcripts of four of these genes were shown to be present during early stages of S. alburnoides male development, suggesting their involvement, at least in the process of hybrid gonad differentiation. The participation of genome-specific gene copies in the global gene expression patterns was assessed by the analysis of six housekeeping and tissue specific genes, revealing the occurrence of a mechanism of non-additive gene expression and allele silencing acting in S. alburnoides triploids, described for the first time in a polyploid vertebrate. Further examination of the evolutionary consequences of the observed patterns of gene expression and the extension of the analysis to representative populatioCoelho, Maria Manuela, 1954-Schartl, Manfred, 1953-Repositório da Universidade de LisboaPala, Irene Alexandra de Carvalho Braz dos Santos, 1981-2010-07-27T08:59:49Z20082008-01-01T00:00:00Zdoctoral thesisinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdftext/xmlhttp://hdl.handle.net/10451/1594porinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T12:39:38Zoai:repositorio.ulisboa.pt:10451/1594Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:25:06.921339Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Functional aspects of hybrid genome interaction : sex determination and gene expression regulation in the Squalius alburnoides complex
title Functional aspects of hybrid genome interaction : sex determination and gene expression regulation in the Squalius alburnoides complex
spellingShingle Functional aspects of hybrid genome interaction : sex determination and gene expression regulation in the Squalius alburnoides complex
Pala, Irene Alexandra de Carvalho Braz dos Santos, 1981-
Hibridação
Poliploidia
Determinação do sexo
Expressão genética
Biologia evolutiva
Teses de doutoramento - 2009
title_short Functional aspects of hybrid genome interaction : sex determination and gene expression regulation in the Squalius alburnoides complex
title_full Functional aspects of hybrid genome interaction : sex determination and gene expression regulation in the Squalius alburnoides complex
title_fullStr Functional aspects of hybrid genome interaction : sex determination and gene expression regulation in the Squalius alburnoides complex
title_full_unstemmed Functional aspects of hybrid genome interaction : sex determination and gene expression regulation in the Squalius alburnoides complex
title_sort Functional aspects of hybrid genome interaction : sex determination and gene expression regulation in the Squalius alburnoides complex
author Pala, Irene Alexandra de Carvalho Braz dos Santos, 1981-
author_facet Pala, Irene Alexandra de Carvalho Braz dos Santos, 1981-
author_role author
dc.contributor.none.fl_str_mv Coelho, Maria Manuela, 1954-
Schartl, Manfred, 1953-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Pala, Irene Alexandra de Carvalho Braz dos Santos, 1981-
dc.subject.por.fl_str_mv Hibridação
Poliploidia
Determinação do sexo
Expressão genética
Biologia evolutiva
Teses de doutoramento - 2009
topic Hibridação
Poliploidia
Determinação do sexo
Expressão genética
Biologia evolutiva
Teses de doutoramento - 2009
description Tese de doutoramento em Biologia (Biologia Evolutiva) apresentada à Universidade de Lisboa através da Faculdade de Ciências, 2009.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2010-07-27T08:59:49Z
dc.type.driver.fl_str_mv doctoral thesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/1594
url http://hdl.handle.net/10451/1594
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
text/xml
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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