Analysis of the inactivation and reactivation kinetics of horseradish peroxidase in mixtures of phosphate buffer/dimethylformamide

Bibliographic Details
Main Author: Machado, M. F.
Publication Date: 2001
Other Authors: Saraiva, J.
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10773/7784
Summary: In this work, the kinetics of the thermal inactivation and reactivation of horseradish peroxidase were analysed in buffered solutions of dimethylformamide at severa1 concentrations (up to 40% vlv) and temperatures ranging fiom 70 to 85 "C. The experimental data of the inactivation and reactivation processes showed a biphasic behaviour, which was well described by a series-type model. The significant activity regain at low temperature of the thermal inactivated enzyme had a strong influence on the estimated value for the a parameter of the model, thus reflecting a clear change of the profile of the experimental curves.
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spelling Analysis of the inactivation and reactivation kinetics of horseradish peroxidase in mixtures of phosphate buffer/dimethylformamideInactivation and reactivation kineticsHorseradish peroxidaseMiixtures of phosphate buffer/dimethylformamideIn this work, the kinetics of the thermal inactivation and reactivation of horseradish peroxidase were analysed in buffered solutions of dimethylformamide at severa1 concentrations (up to 40% vlv) and temperatures ranging fiom 70 to 85 "C. The experimental data of the inactivation and reactivation processes showed a biphasic behaviour, which was well described by a series-type model. The significant activity regain at low temperature of the thermal inactivated enzyme had a strong influence on the estimated value for the a parameter of the model, thus reflecting a clear change of the profile of the experimental curves.Universidade de Aveiro2012-04-05T09:56:43Z2001-01-01T00:00:00Z2001conference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10773/7784eng972-789-041-5Machado, M. F.Saraiva, J.info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-06T03:40:05Zoai:ria.ua.pt:10773/7784Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T13:42:36.056373Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Analysis of the inactivation and reactivation kinetics of horseradish peroxidase in mixtures of phosphate buffer/dimethylformamide
title Analysis of the inactivation and reactivation kinetics of horseradish peroxidase in mixtures of phosphate buffer/dimethylformamide
spellingShingle Analysis of the inactivation and reactivation kinetics of horseradish peroxidase in mixtures of phosphate buffer/dimethylformamide
Machado, M. F.
Inactivation and reactivation kinetics
Horseradish peroxidase
Miixtures of phosphate buffer/dimethylformamide
title_short Analysis of the inactivation and reactivation kinetics of horseradish peroxidase in mixtures of phosphate buffer/dimethylformamide
title_full Analysis of the inactivation and reactivation kinetics of horseradish peroxidase in mixtures of phosphate buffer/dimethylformamide
title_fullStr Analysis of the inactivation and reactivation kinetics of horseradish peroxidase in mixtures of phosphate buffer/dimethylformamide
title_full_unstemmed Analysis of the inactivation and reactivation kinetics of horseradish peroxidase in mixtures of phosphate buffer/dimethylformamide
title_sort Analysis of the inactivation and reactivation kinetics of horseradish peroxidase in mixtures of phosphate buffer/dimethylformamide
author Machado, M. F.
author_facet Machado, M. F.
Saraiva, J.
author_role author
author2 Saraiva, J.
author2_role author
dc.contributor.author.fl_str_mv Machado, M. F.
Saraiva, J.
dc.subject.por.fl_str_mv Inactivation and reactivation kinetics
Horseradish peroxidase
Miixtures of phosphate buffer/dimethylformamide
topic Inactivation and reactivation kinetics
Horseradish peroxidase
Miixtures of phosphate buffer/dimethylformamide
description In this work, the kinetics of the thermal inactivation and reactivation of horseradish peroxidase were analysed in buffered solutions of dimethylformamide at severa1 concentrations (up to 40% vlv) and temperatures ranging fiom 70 to 85 "C. The experimental data of the inactivation and reactivation processes showed a biphasic behaviour, which was well described by a series-type model. The significant activity regain at low temperature of the thermal inactivated enzyme had a strong influence on the estimated value for the a parameter of the model, thus reflecting a clear change of the profile of the experimental curves.
publishDate 2001
dc.date.none.fl_str_mv 2001-01-01T00:00:00Z
2001
2012-04-05T09:56:43Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/7784
url http://hdl.handle.net/10773/7784
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 972-789-041-5
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de Aveiro
publisher.none.fl_str_mv Universidade de Aveiro
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833593995445403648

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