Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia

Bibliographic Details
Main Author: Almeida, J. P. Lopes de
Publication Date: 2012
Other Authors: Freitas-Santos, T., Saldanha, C.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/10677
Summary: © 2012 – IOS Press and the authors. All rights reserved
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spelling Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemiaBand 3 proteinErythrocyteDeformabilityFibrinogen© 2012 – IOS Press and the authors. All rights reservedRecent evidence has shown that plasma fibrinogen, a major cardiovascular risk factor, interacts with the erythrocyte membrane and acts to influence blood flow via erythrocyte nitric oxide (NO) modulation. In the present in-vitro study, whole blood samples were harvested from healthy subjects and aliquots were incubated in the absence (control aliquots) and presence of fibrinogen at different degrees of band 3 phosphorylation, and the erythrocyte deformability was determined. The present study shows that in the presence of higher fibrinogen concentrations, similar to those found in inflammatory conditions, erythrocyte deformability is increased only when band 3 is dephosphorylated by the presence of syk inhibitor and at low shear stress. On the contrary, no changes were verified in the presence of fibrinogen when band 3 is allowed to be phosphorylated by inhibiting the phosphotyrosine phosphatase enzyme activity with calpeptin. We also observed that the presence of fibrinogen at higher concentration does not induce changes in erythrocyte deformability in the absence of modulators of the band 3 phosphorylation degree. However, the mechanisms by which fibrinogen signalling modulates erythrocyte function remain to be clarified and are currently under study.IOS PressRepositório da Universidade de LisboaAlmeida, J. P. Lopes deFreitas-Santos, T.Saldanha, C.2014-03-05T10:54:34Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10677engClinical Hemorheology and Microcirculation 50 (2012) 213–2191386-0291http://dx.doi.org/10.3233/CH-2010-1433metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:06:35Zoai:repositorio.ulisboa.pt:10451/10677Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:35:07.461536Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia
title Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia
spellingShingle Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia
Almeida, J. P. Lopes de
Band 3 protein
Erythrocyte
Deformability
Fibrinogen
title_short Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia
title_full Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia
title_fullStr Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia
title_full_unstemmed Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia
title_sort Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia
author Almeida, J. P. Lopes de
author_facet Almeida, J. P. Lopes de
Freitas-Santos, T.
Saldanha, C.
author_role author
author2 Freitas-Santos, T.
Saldanha, C.
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Almeida, J. P. Lopes de
Freitas-Santos, T.
Saldanha, C.
dc.subject.por.fl_str_mv Band 3 protein
Erythrocyte
Deformability
Fibrinogen
topic Band 3 protein
Erythrocyte
Deformability
Fibrinogen
description © 2012 – IOS Press and the authors. All rights reserved
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-03-05T10:54:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10677
url http://hdl.handle.net/10451/10677
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Hemorheology and Microcirculation 50 (2012) 213–219
1386-0291
http://dx.doi.org/10.3233/CH-2010-1433
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv IOS Press
publisher.none.fl_str_mv IOS Press
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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