Serum multi-omics analysis in hindlimb unloading mice model
| Main Author: | |
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| Publication Date: | 2024 |
| Other Authors: | , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | http://hdl.handle.net/10362/163798 |
Summary: | © 2023 The Authors. |
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Serum multi-omics analysis in hindlimb unloading mice modelInsights into systemic molecular changes and potential diagnostic and therapeutic biomarkersHindlimb unloadingCardiovascularSkeletal muscleDeconditioningMetabolomicProteomicBiomarkersSDG 3 - Good Health and Well-being© 2023 The Authors.Microgravity, in space travel and prolonged bed rest conditions, induces cardiovascular deconditioning along with skeletal muscle mass loss and weakness. The findings of microgravity research may also aid in the understanding and treatment of human health conditions on Earth such as muscle atrophy, and cardiovascular diseases. Due to the paucity of biomarkers and the unknown underlying mechanisms of cardiovascular and skeletal muscle deconditioning in these environments, there are insufficient diagnostic and preventative measures. In this study, we employed hindlimb unloading (HU) mouse model, which mimics astronauts in space and bedridden patients, to first evaluate cardiovascular and skeletal muscle function, followed by proteomics and metabolomics LC-MS/MS-based analysis using serum samples. Three weeks of unloading caused changes in the function of the cardiovascular system in c57/Bl6 mice, as seen by a decrease in mean arterial pressure and heart weight. Unloading for three weeks also changed skeletal muscle function, causing a loss in grip strength in HU mice and atrophy of skeletal muscle indicated by a reduction in muscle mass. These modifications were partially reversed by a two-week recovery period of reloading condition, emphasizing the significance of the recovery process. Proteomics analysis revealed 12 dysregulated proteins among the groups, such as phospholipid transfer protein, Carbonic anhydrase 3, Parvalbumin alpha, Major urinary protein 20 (Mup20), Thrombospondin-1, and Apolipoprotein C-IV. On the other hand, metabolomics analysis showed altered metabolites among the groups such as inosine, hypoxanthine, xanthosine, sphinganine, l-valine, 3,4-Dihydroxyphenylglycol, and l-Glutamic acid. The joint data analysis revealed that HU conditions mainly impacted pathways such as ABC transporters, complement and coagulation cascades, nitrogen metabolism, and purine metabolism. Overall, our results indicate that microgravity environment induces significant alterations in the function, proteins, and metabolites of these mice. These observations suggest the potential utilization of these proteins and metabolites as novel biomarkers for assessing and mitigating cardiovascular and skeletal muscle deconditioning associated with such conditions.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centre for Toxicogenomics and Human Health (ToxOmics)RUNIbrahim, ZeinabKhan, Naveed AQaisar, RizwanSaleh, Mohamed ASiddiqui, RuqaiyyahAl-Hroub, Hamza MGiddey, Alexander DSemreen, Mohammad HarbSoares, Nelson CElmoselhi, Adel B2024-02-19T23:46:13Z2024-01-152024-01-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/163798eng2405-8440PURE: 82946047https://doi.org/10.1016/j.heliyon.2023.e23592info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:18:36Zoai:run.unl.pt:10362/163798Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:49:24.737038Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Serum multi-omics analysis in hindlimb unloading mice model Insights into systemic molecular changes and potential diagnostic and therapeutic biomarkers |
| title |
Serum multi-omics analysis in hindlimb unloading mice model |
| spellingShingle |
Serum multi-omics analysis in hindlimb unloading mice model Ibrahim, Zeinab Hindlimb unloading Cardiovascular Skeletal muscle Deconditioning Metabolomic Proteomic Biomarkers SDG 3 - Good Health and Well-being |
| title_short |
Serum multi-omics analysis in hindlimb unloading mice model |
| title_full |
Serum multi-omics analysis in hindlimb unloading mice model |
| title_fullStr |
Serum multi-omics analysis in hindlimb unloading mice model |
| title_full_unstemmed |
Serum multi-omics analysis in hindlimb unloading mice model |
| title_sort |
Serum multi-omics analysis in hindlimb unloading mice model |
| author |
Ibrahim, Zeinab |
| author_facet |
Ibrahim, Zeinab Khan, Naveed A Qaisar, Rizwan Saleh, Mohamed A Siddiqui, Ruqaiyyah Al-Hroub, Hamza M Giddey, Alexander D Semreen, Mohammad Harb Soares, Nelson C Elmoselhi, Adel B |
| author_role |
author |
| author2 |
Khan, Naveed A Qaisar, Rizwan Saleh, Mohamed A Siddiqui, Ruqaiyyah Al-Hroub, Hamza M Giddey, Alexander D Semreen, Mohammad Harb Soares, Nelson C Elmoselhi, Adel B |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centre for Toxicogenomics and Human Health (ToxOmics) RUN |
| dc.contributor.author.fl_str_mv |
Ibrahim, Zeinab Khan, Naveed A Qaisar, Rizwan Saleh, Mohamed A Siddiqui, Ruqaiyyah Al-Hroub, Hamza M Giddey, Alexander D Semreen, Mohammad Harb Soares, Nelson C Elmoselhi, Adel B |
| dc.subject.por.fl_str_mv |
Hindlimb unloading Cardiovascular Skeletal muscle Deconditioning Metabolomic Proteomic Biomarkers SDG 3 - Good Health and Well-being |
| topic |
Hindlimb unloading Cardiovascular Skeletal muscle Deconditioning Metabolomic Proteomic Biomarkers SDG 3 - Good Health and Well-being |
| description |
© 2023 The Authors. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-02-19T23:46:13Z 2024-01-15 2024-01-15T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/163798 |
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http://hdl.handle.net/10362/163798 |
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eng |
| language |
eng |
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2405-8440 PURE: 82946047 https://doi.org/10.1016/j.heliyon.2023.e23592 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
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RCAAP |
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RCAAP |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
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info@rcaap.pt |
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