Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies

Bibliographic Details
Main Author: Beldade, P.
Publication Date: 2009
Other Authors: Van´T Hof, Arje`n E., Jerónimo, MA., Long, AD.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.7/195
Summary: Deriving useful microsatellite markers in lepidopterans has been challenging when relying on scans of genomic DNA libraries, presumably due to repetitiveness in their genomes. We assayed 96 of 320 microsatellites identified in silico from a collection of Bicyclus anynana ESTs, in 11 independent individuals from a laboratory population.From the 68 successful assays, we identified 40 polymorphic markers including 22 with BLAST-based annotation. Nine of 12 selected polymorphic markers tested in a panel of 24 wild-caught individuals converted to successful assays and were all polymorphic. We discuss how microsatellite discovery in ESTs is an efficient strategy with important attendant advantages.
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spelling Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterfliesBicyclus anynanaESTGenetic markersLepidopteraMicrosatellitePolymorphismDeriving useful microsatellite markers in lepidopterans has been challenging when relying on scans of genomic DNA libraries, presumably due to repetitiveness in their genomes. We assayed 96 of 320 microsatellites identified in silico from a collection of Bicyclus anynana ESTs, in 11 independent individuals from a laboratory population.From the 68 successful assays, we identified 40 polymorphic markers including 22 with BLAST-based annotation. Nine of 12 selected polymorphic markers tested in a panel of 24 wild-caught individuals converted to successful assays and were all polymorphic. We discuss how microsatellite discovery in ESTs is an efficient strategy with important attendant advantages.BlackwellARCABeldade, P.Van´T Hof, Arje`n E.Jerónimo, MA.Long, AD.2010-08-30T09:37:40Z2009-022009-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/195eng1755-098Xinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-11-21T14:19:10Zoai:arca.igc.gulbenkian.pt:10400.7/195Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T19:14:35.465451Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies
title Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies
spellingShingle Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies
Beldade, P.
Bicyclus anynana
EST
Genetic markers
Lepidoptera
Microsatellite
Polymorphism
title_short Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies
title_full Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies
title_fullStr Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies
title_full_unstemmed Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies
title_sort Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies
author Beldade, P.
author_facet Beldade, P.
Van´T Hof, Arje`n E.
Jerónimo, MA.
Long, AD.
author_role author
author2 Van´T Hof, Arje`n E.
Jerónimo, MA.
Long, AD.
author2_role author
author
author
dc.contributor.none.fl_str_mv ARCA
dc.contributor.author.fl_str_mv Beldade, P.
Van´T Hof, Arje`n E.
Jerónimo, MA.
Long, AD.
dc.subject.por.fl_str_mv Bicyclus anynana
EST
Genetic markers
Lepidoptera
Microsatellite
Polymorphism
topic Bicyclus anynana
EST
Genetic markers
Lepidoptera
Microsatellite
Polymorphism
description Deriving useful microsatellite markers in lepidopterans has been challenging when relying on scans of genomic DNA libraries, presumably due to repetitiveness in their genomes. We assayed 96 of 320 microsatellites identified in silico from a collection of Bicyclus anynana ESTs, in 11 independent individuals from a laboratory population.From the 68 successful assays, we identified 40 polymorphic markers including 22 with BLAST-based annotation. Nine of 12 selected polymorphic markers tested in a panel of 24 wild-caught individuals converted to successful assays and were all polymorphic. We discuss how microsatellite discovery in ESTs is an efficient strategy with important attendant advantages.
publishDate 2009
dc.date.none.fl_str_mv 2009-02
2009-02-01T00:00:00Z
2010-08-30T09:37:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.7/195
url http://hdl.handle.net/10400.7/195
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1755-098X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Blackwell
publisher.none.fl_str_mv Blackwell
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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