Activation of adenosine A2A receptor facilitates brain-derived neurotrophic factor modulation of synaptic

Bibliographic Details
Main Author: Diógenes, Maria José
Publication Date: 2004
Other Authors: Fernandes, Catarina Cunha, Sebastião, Ana M, Ribeiro, Joaquim A.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/7505
Summary: ©2004 Society for Neuroscience
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spelling Activation of adenosine A2A receptor facilitates brain-derived neurotrophic factor modulation of synapticAdenosine receptorsBrain-derived neurotrophic factor (BDNF)HippocampusSynaptic transmissionNeuromodulationNeurotrophic factors©2004 Society for NeuroscienceBoth brain-derived neurotrophic factor (BDNF) and adenosine influence neuronal plasticity. We now investigated how adenosine influences the action of BDNF on synaptic transmission in the CA1 area of the rat hippocampal slices. Alone, BDNF (20 –100 ng/ml) did not significantly affect field EPSPs (fEPSPs). However, a 2 min pulse of high-K+ (10 mM) 46 min before the application of BDNF (20 ng/ml)triggered an excitatory action, an effect blocked by the TrkB receptor inhibitor K252a (200 nM), by the adenosine A2A receptor antagonist ZM241385 (50 nM), and by the protein kinaseAinhibitor H-89 (1µM). Presynaptic, rather than postsynaptic depolarization was required to trigger the BDNF action because after K+ depolarization BDNF also increased EPSCs recorded from pyramidal neurons voltageclamped at -60 mV, and transient postsynaptic depolarization was unable to unmask the BDNF action. A weak theta burst stimulation of the afferents could elicit potentiation of synaptic transmission only when applied in the presence of BDNF. Activation of adenosineA2A receptors with CGS 21680 (10 nM), or the increase in extracellular adenosine levels induced by 5-iodotubercidin (100 nM) triggered the excitatory action of BDNF, a process prevented by ZM 241385 and by H-89. In the presence of dibutyryl-cAMP (0.5 mM), BDNF also increased fEPSPs but postsynaptic cAMP (0.5mM) was unable to trigger the BDNF action. It is concluded that presynaptic activity-dependent release of adenosine, through activation of A2A receptors, facilitates BDNF modulation of synaptic transmission at hippocampal synapses.This work was supported by Fundação para a Ciência e TecnologiaSociety for NeuroscienceRepositório da Universidade de LisboaDiógenes, Maria JoséFernandes, Catarina CunhaSebastião, Ana MRibeiro, Joaquim A.2013-01-15T15:29:21Z20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/7505engThe Journal of Neuroscience, March 24, 2004 • 24(12):2905–29130270-6474http://dx.doi.org/10.1523/JNEUROSCI.4454-03.2004info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T12:56:13Zoai:repositorio.ulisboa.pt:10451/7505Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:31:24.310453Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Activation of adenosine A2A receptor facilitates brain-derived neurotrophic factor modulation of synaptic
title Activation of adenosine A2A receptor facilitates brain-derived neurotrophic factor modulation of synaptic
spellingShingle Activation of adenosine A2A receptor facilitates brain-derived neurotrophic factor modulation of synaptic
Diógenes, Maria José
Adenosine receptors
Brain-derived neurotrophic factor (BDNF)
Hippocampus
Synaptic transmission
Neuromodulation
Neurotrophic factors
title_short Activation of adenosine A2A receptor facilitates brain-derived neurotrophic factor modulation of synaptic
title_full Activation of adenosine A2A receptor facilitates brain-derived neurotrophic factor modulation of synaptic
title_fullStr Activation of adenosine A2A receptor facilitates brain-derived neurotrophic factor modulation of synaptic
title_full_unstemmed Activation of adenosine A2A receptor facilitates brain-derived neurotrophic factor modulation of synaptic
title_sort Activation of adenosine A2A receptor facilitates brain-derived neurotrophic factor modulation of synaptic
author Diógenes, Maria José
author_facet Diógenes, Maria José
Fernandes, Catarina Cunha
Sebastião, Ana M
Ribeiro, Joaquim A.
author_role author
author2 Fernandes, Catarina Cunha
Sebastião, Ana M
Ribeiro, Joaquim A.
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Diógenes, Maria José
Fernandes, Catarina Cunha
Sebastião, Ana M
Ribeiro, Joaquim A.
dc.subject.por.fl_str_mv Adenosine receptors
Brain-derived neurotrophic factor (BDNF)
Hippocampus
Synaptic transmission
Neuromodulation
Neurotrophic factors
topic Adenosine receptors
Brain-derived neurotrophic factor (BDNF)
Hippocampus
Synaptic transmission
Neuromodulation
Neurotrophic factors
description ©2004 Society for Neuroscience
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01T00:00:00Z
2013-01-15T15:29:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/7505
url http://hdl.handle.net/10451/7505
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv The Journal of Neuroscience, March 24, 2004 • 24(12):2905–2913
0270-6474
http://dx.doi.org/10.1523/JNEUROSCI.4454-03.2004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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