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Expression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approach

Bibliographic Details
Main Author: Bessa, Paulo C.
Publication Date: 2006
Other Authors: Pedro, A. P., Nobre, Alexandra, Klosch, B., Redl, Heinz, Reis, R. L., Casal, Margarida
Language: por
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/54019
Summary: Bone morphogenetic proteins have promoted great biomedical interest due to their ability in inducing new bone formation when used as powerful osteoinductive components of several late-stage bone grafting products. Recombinant human bone morphogenetic protein-2 (rhBMP-2) is obtained from mammalian cell expressing systems in low amounts or from bacteria inclusion bodies after timeconsuming refolding methods. Thus, there is a need to establish novel approaches for producing rhBMP-2 in high yields by simple and cheap procedures.
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spelling Expression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approachBoneProteinBioactivityCiências Naturais::Ciências BiológicasEngenharia e Tecnologia::Engenharia MédicaBone morphogenetic proteins have promoted great biomedical interest due to their ability in inducing new bone formation when used as powerful osteoinductive components of several late-stage bone grafting products. Recombinant human bone morphogenetic protein-2 (rhBMP-2) is obtained from mammalian cell expressing systems in low amounts or from bacteria inclusion bodies after timeconsuming refolding methods. Thus, there is a need to establish novel approaches for producing rhBMP-2 in high yields by simple and cheap procedures.Portuguese Foundation for Science and Technolog y, FCT (PhD Grant to PC Bessa , SFRH/BD/17049/2004). This work was also partially supported by the European STREP HIPPOCRATES (NMP3 - CT - 2003 - 505758) and carried out under the scope of European NoE EXPERTISSUES (NMP3 - CT - 2004 - 500283).info:eu-repo/semantics/publishedVersionUniversidade do MinhoBessa, Paulo C.Pedro, A. P.Nobre, AlexandraKlosch, B.Redl, HeinzReis, R. L.Casal, Margarida20062006-01-01T00:00:00Zconference posterinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/54019porinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T05:45:02Zoai:repositorium.sdum.uminho.pt:1822/54019Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:28:47.690532Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Expression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approach
title Expression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approach
spellingShingle Expression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approach
Bessa, Paulo C.
Bone
Protein
Bioactivity
Ciências Naturais::Ciências Biológicas
Engenharia e Tecnologia::Engenharia Médica
title_short Expression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approach
title_full Expression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approach
title_fullStr Expression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approach
title_full_unstemmed Expression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approach
title_sort Expression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approach
author Bessa, Paulo C.
author_facet Bessa, Paulo C.
Pedro, A. P.
Nobre, Alexandra
Klosch, B.
Redl, Heinz
Reis, R. L.
Casal, Margarida
author_role author
author2 Pedro, A. P.
Nobre, Alexandra
Klosch, B.
Redl, Heinz
Reis, R. L.
Casal, Margarida
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Bessa, Paulo C.
Pedro, A. P.
Nobre, Alexandra
Klosch, B.
Redl, Heinz
Reis, R. L.
Casal, Margarida
dc.subject.por.fl_str_mv Bone
Protein
Bioactivity
Ciências Naturais::Ciências Biológicas
Engenharia e Tecnologia::Engenharia Médica
topic Bone
Protein
Bioactivity
Ciências Naturais::Ciências Biológicas
Engenharia e Tecnologia::Engenharia Médica
description Bone morphogenetic proteins have promoted great biomedical interest due to their ability in inducing new bone formation when used as powerful osteoinductive components of several late-stage bone grafting products. Recombinant human bone morphogenetic protein-2 (rhBMP-2) is obtained from mammalian cell expressing systems in low amounts or from bacteria inclusion bodies after timeconsuming refolding methods. Thus, there is a need to establish novel approaches for producing rhBMP-2 in high yields by simple and cheap procedures.
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference poster
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/54019
url http://hdl.handle.net/1822/54019
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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