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Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells

Bibliographic Details
Main Author: Mihaila, Silvia M.
Publication Date: 2017
Other Authors: Resende, Margarida F., Reis, R. L., Gomes, Manuela E., Marques, A. P.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/41904
Summary: "Version of Record online: 29 OCT 2015"
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spelling Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cellsCo-cultureSSEA-4-positive hASCsEndothelial cellsOsteoblast-like cellsBoneVascularizationScience & Technology"Version of Record online: 29 OCT 2015"Bone formation relies on complex processes that require well-orchestrated interactions between several cell types, such as bone-forming cells (osteoblasts, OBs) and endothelial cells (ECs). Their co-culture has been proved relevant to mimicking specific features of the bone niche. Here we propose the co-culture of microvascular-like ECs and pre-OBs, both derived from the SSEA-4+ cell subpopulation from the stromal vascular fraction of human adipose tissue (SSEA-4+ hASCs), to define the conditions in which cells synergistically communicate to support the full differentiation of pre-OBs and maintenance of the EC phenotype. Co-cultures of different ratios of the two cell types were established and maintained for up to 21 days in standard endothelial maintenance (ENDO) and osteogenic differentiation (OST) media, as well as in a mixture of these (MIX). The osteogenic maturation of pre-OBs (ALP activity, OPN and OCN expression, calcium deposition), the evolution of EC numbers (CD31+ cells) and maintenance of the endothelial phenotype (CD31 and vWF expression, LDL uptake) were assessed throughout the culture time as a function of cell ratio and culture media. The results obtained demonstrate that EC number has a significant effect on the osteogenic differentiation of pre-OBs, depending on the medium used. While in ENDO medium the osteogenic differentiation was not observed, in the OST and MIX media it was attained at similar levels, except for the co-culture with a higher number of ECs in MIX medium. These findings demonstrate that the use of SSEA-4+ hASCs as a single-cell source is promising to attain 3D bone-like models with the potential to promote vascularized bone tissue regeneration.The authors are grateful to the Portuguese Foundation for Science and Technology (FCT) for personal grants SFRH/BD/ 42968/2008 through the MIT–Portugal Programme (SMM). The research leading to these results has received funding from the MIT/ECE/0047/2009 project, the European Union Seventh Framework Programme (Grant No. FP7/2007-2013, under Grant Agreement No. REGPOT-CT2012-316331-POLARIS) and the European Research Council (Advanced Grant No. ERC-2012- AdG_20120216-321266 for the project ComplexiTE).John Wiley and SonsUniversidade do MinhoMihaila, Silvia M.Resende, Margarida F.Reis, R. L.Gomes, Manuela E.Marques, A. P.20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/41904engMihaila S. M., Resende M., Reis R. L., Gomes M. E., Marques A. P. Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells, Journal of Tissue Engineering and Regenerative Medicine, doi:10.1002/term.2096, 20151932-700510.1002/term.209626510831http://onlinelibrary.wiley.com/doi/10.1002/term.2096/fullinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T06:58:27Zoai:repositorium.sdum.uminho.pt:1822/41904Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:10:53.197292Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells
title Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells
spellingShingle Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells
Mihaila, Silvia M.
Co-culture
SSEA-4-positive hASCs
Endothelial cells
Osteoblast-like cells
Bone
Vascularization
Science & Technology
title_short Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells
title_full Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells
title_fullStr Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells
title_full_unstemmed Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells
title_sort Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells
author Mihaila, Silvia M.
author_facet Mihaila, Silvia M.
Resende, Margarida F.
Reis, R. L.
Gomes, Manuela E.
Marques, A. P.
author_role author
author2 Resende, Margarida F.
Reis, R. L.
Gomes, Manuela E.
Marques, A. P.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Mihaila, Silvia M.
Resende, Margarida F.
Reis, R. L.
Gomes, Manuela E.
Marques, A. P.
dc.subject.por.fl_str_mv Co-culture
SSEA-4-positive hASCs
Endothelial cells
Osteoblast-like cells
Bone
Vascularization
Science & Technology
topic Co-culture
SSEA-4-positive hASCs
Endothelial cells
Osteoblast-like cells
Bone
Vascularization
Science & Technology
description "Version of Record online: 29 OCT 2015"
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/41904
url http://hdl.handle.net/1822/41904
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mihaila S. M., Resende M., Reis R. L., Gomes M. E., Marques A. P. Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells, Journal of Tissue Engineering and Regenerative Medicine, doi:10.1002/term.2096, 2015
1932-7005
10.1002/term.2096
26510831
http://onlinelibrary.wiley.com/doi/10.1002/term.2096/full
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595787559305216

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