The two alternative NADH: quinone oxidoreductases from Staphylococcus aureus

Bibliographic Details
Main Author: Sena, Filipa V.
Publication Date: 2024
Other Authors: Sousa, Filipe M., Pereira, Ana R., Catarino, Teresa, Cabrita, Eurico J., Pinho, Mariana G., Pinto, Francisco R., Pereira, Manuela M.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/174200
Summary: Funding Information: F.V.S. and F.M.S. were recipients of fellowships by Fundação para a Ciência e a Tecnologia within the scope of the PhD program Molecular Biosciences PD/00133/2012. Publisher Copyright: © 2024 Sena et al.
id RCAP_0cb7d8a4d36835a8d2088df6fc0b266a
oai_identifier_str oai:run.unl.pt:10362/174200
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling The two alternative NADH: quinone oxidoreductases from Staphylococcus aureustwo players with differentmolecular and cellular rolesalternative NADH oxidasecharge-transfer complexmembrane proteinsmonotopic proteinsNAD(P)Hquinonesrespiratory chainPhysiologyEcologyImmunology and Microbiology(all)GeneticsMicrobiology (medical)Cell BiologyInfectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: F.V.S. and F.M.S. were recipients of fellowships by Fundação para a Ciência e a Tecnologia within the scope of the PhD program Molecular Biosciences PD/00133/2012. Publisher Copyright: © 2024 Sena et al.Staphylococcus aureus is an opportunistic pathogen that has emerged as a major public health threat due to the increased incidence of its drug resistance. S. aureus presents a remarkable capacity to adapt to differentniches due to the plasticity of its energy metabolism. In this work, we investigated the energy metabolism of S. aureus, focusing on the alternative NADH:quinone oxidoreductases, NDH-2s. S. aureus presents two genes encoding NDH-2s (NDH-2A and NDH-2B) and lacks genes coding for Complex I, the canonical respiratory NADH:quinone oxidoreductase. This observation makes the action of NDH-2s crucial for the regeneration of NAD+ and, consequently, for the progression of metabolism. Our study involved the comprehensive biochemical characterization of NDH-2B and the exploration of the cellular roles of NDH-2A and NDH-2B, utilizing knockout mutants (Δndh-2a and Δndh-2b). We show that NDH-2B uses NADPH instead of NADH, does not establish a charge-transfer complex in the presence of NADPH, and its reduction by this substrate is the catalytic rate-limiting step. In the case of NDH-2B, the reduction of the flavinis inherently slow, and we suggest the establishment of a charge transfer complex between NADP+ and FADH2, as previously observed for NDH-2A, to slow down quinone reduction and, consequently, prevent the overproduction of reactive oxygen species, which is potentially unnecessary. Furthermore, we observed that the lack of NDH-2A or NDH-2B impacts cell growth, volume, and division differently.The absence of these enzymes results in distinct metabolic phenotypes, emphasizing the unique cellular roles of each NDH-2 in energy metabolism.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNSena, Filipa V.Sousa, Filipe M.Pereira, Ana R.Catarino, TeresaCabrita, Eurico J.Pinho, Mariana G.Pinto, Francisco R.Pereira, Manuela M.2024-10-29T00:33:41Z2024-082024-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article24application/pdfhttp://hdl.handle.net/10362/174200engSena, F. V., Sousa, F. M., Pereira, A. R., Catarino, T., Cabrita, E. J., Pinho, M. G., Pinto, F. R., & Pereira, M. M. (2024). The two alternative NADH: quinone oxidoreductases from Staphylococcus aureus: two players with differentmolecular and cellular roles. Microbiology Spectrum, 12(8). Advance online publication. https://doi.org/10.1128/spectrum.04152-232165-0497PURE: 99131336https://doi.org/10.1128/spectrum.04152-23info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-01-20T01:36:57Zoai:run.unl.pt:10362/174200Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T19:12:35.300105Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv The two alternative NADH: quinone oxidoreductases from Staphylococcus aureus
two players with differentmolecular and cellular roles
title The two alternative NADH: quinone oxidoreductases from Staphylococcus aureus
spellingShingle The two alternative NADH: quinone oxidoreductases from Staphylococcus aureus
Sena, Filipa V.
alternative NADH oxidase
charge-transfer complex
membrane proteins
monotopic proteins
NAD(P)H
quinones
respiratory chain
Physiology
Ecology
Immunology and Microbiology(all)
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short The two alternative NADH: quinone oxidoreductases from Staphylococcus aureus
title_full The two alternative NADH: quinone oxidoreductases from Staphylococcus aureus
title_fullStr The two alternative NADH: quinone oxidoreductases from Staphylococcus aureus
title_full_unstemmed The two alternative NADH: quinone oxidoreductases from Staphylococcus aureus
title_sort The two alternative NADH: quinone oxidoreductases from Staphylococcus aureus
author Sena, Filipa V.
author_facet Sena, Filipa V.
Sousa, Filipe M.
Pereira, Ana R.
Catarino, Teresa
Cabrita, Eurico J.
Pinho, Mariana G.
Pinto, Francisco R.
Pereira, Manuela M.
author_role author
author2 Sousa, Filipe M.
Pereira, Ana R.
Catarino, Teresa
Cabrita, Eurico J.
Pinho, Mariana G.
Pinto, Francisco R.
Pereira, Manuela M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Sena, Filipa V.
Sousa, Filipe M.
Pereira, Ana R.
Catarino, Teresa
Cabrita, Eurico J.
Pinho, Mariana G.
Pinto, Francisco R.
Pereira, Manuela M.
dc.subject.por.fl_str_mv alternative NADH oxidase
charge-transfer complex
membrane proteins
monotopic proteins
NAD(P)H
quinones
respiratory chain
Physiology
Ecology
Immunology and Microbiology(all)
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases
SDG 3 - Good Health and Well-being
topic alternative NADH oxidase
charge-transfer complex
membrane proteins
monotopic proteins
NAD(P)H
quinones
respiratory chain
Physiology
Ecology
Immunology and Microbiology(all)
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases
SDG 3 - Good Health and Well-being
description Funding Information: F.V.S. and F.M.S. were recipients of fellowships by Fundação para a Ciência e a Tecnologia within the scope of the PhD program Molecular Biosciences PD/00133/2012. Publisher Copyright: © 2024 Sena et al.
publishDate 2024
dc.date.none.fl_str_mv 2024-10-29T00:33:41Z
2024-08
2024-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/174200
url http://hdl.handle.net/10362/174200
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sena, F. V., Sousa, F. M., Pereira, A. R., Catarino, T., Cabrita, E. J., Pinho, M. G., Pinto, F. R., & Pereira, M. M. (2024). The two alternative NADH: quinone oxidoreductases from Staphylococcus aureus: two players with differentmolecular and cellular roles. Microbiology Spectrum, 12(8). Advance online publication. https://doi.org/10.1128/spectrum.04152-23
2165-0497
PURE: 99131336
https://doi.org/10.1128/spectrum.04152-23
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 24
application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833597942829678592

Similar Items