Metabolic profiling of renal cell carcinoma tissue using gas chromatography metabolomics

• Autor(a) principal: Amaro, Filipa
• Data de Publicação: 2024
• Outros Autores: Carvalho, Márcia, Carvalho-Maia, Carina, Jerónimo, Carmen, Henrique, Rui, Bastos, Maria de Lourdes, Guedes de Pinho, Paula, Pinto, Joana
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
• Texto Completo: https://doi.org/10.48797/sl.2024.243
• topic: Poster

Background: Renal cell carcinoma (RCC) is marked by dysregulation of angiogenesis, energy metabolism, and nutrient sensing pathways [1]. This diversity is an obstacle to achieving long-term responses to treatment, notwithstanding progress in targeted and immunotherapeutic drugs. Objective: This study aimed to characterise the metabolic dysregulations that occur in RCC tissue using a metabolomics approach. Methods: Tumour and non-tumour kidney tissues were collected from 18 patients who underwent nephrectomy at the Portuguese Oncology Institute of Porto (IPO-Porto). Ethical approval (238/2018) and written consent were obtained. Tissues were homogenised, and metabolites were extracted using a methanol-water technique. Metabolites were then analysed by gas chromatography-mass spectrometry (GC-MS) analysis. Statistical methods and pathway analysis were used to interpret potential dysregulations associated with RCC. Results: RCC tissue showed a significant reduction in amino acid levels (including alanine, asparagine, aspartate, serine, tyrosine, among others), except for β-alanine and glutamate, which exhibited significant elevated levels. Perturbations in organic acids were observed, with a significant decrease in fumarate and gluconate levels and an increase in 3-aminobutyrate, citrate, and lactate. Increased levels of glucose and maltose were also found in RCC tissue, whereas sugar derivatives such as myo-inositol and scyllo-inositol showed decreased levels. Pathway analysis suggested dysregulation in amino acid, energy (TCA cycle, pyruvate metabolism), sugar, and glutathione metabolism pathways in RCC tissue. Conclusions: These results reveal the metabolic reprogramming related with the development and progression of RCC. Understanding these alterations provides important insights for improving RCC treatment strategies.