Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration

Detalhes bibliográficos
Autor(a) principal: Escobar Fernandez, Ane
Data de Publicação: 2023
Outros Autores: Carvalho, Mariana R., Silva, Tiago H., Reis, R. L., Oliveira, Joaquim M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: https://hdl.handle.net/1822/85736
Resumo: Published 18 April 2023
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spelling Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regenerationConduitsPeripheral Nerve Regeneration (PNR)Silk FibroinNGCsPublished 18 April 2023Peripheral nerve injuries represent a major clinical challenge, if nerve ends retract, there is no spontaneous regeneration, and grafts are required to proximate the nerve ends and give continuity to the nerve. The nerve guidance conduits (NGCs) presented in this work are silk fibroin (SF)-based, which is biocompatible and very versatile. The formation of conduits is obtained by forming a covalently cross-linked hydrogel in two concentric moulds, and the inner longitudinally aligned pattern of the SF NGCs is obtained through the use of a patterned inner mould. SF NGCs with two wall thicknesses of ~ 200 to ~ 400 μm are synthesized. Their physicochemical and mechanical characteristics have shown improved properties when the wall thickness is thicker such as resistance to kinking, which is of special importance as conduits might also be used to substitute nerves in flexible body parts. The Young modulus is higher for conduits with inner pattern, and none of the conduits has shown any salt deposition in presence of simulated body fluid, meaning they do not calcify; thus, the regeneration does not get impaired when conduits have contact with body fluids. In vitro studies demonstrated the biocompatibility of the SF NGCs; proliferation is enhanced when iSCs are cultured on top of conduits with longitudinally aligned pattern. BJ fibroblasts cannot infiltrate through the SF wall, avoiding scar tissue formation on the lumen of the graft when used in vivo. These conduits have been demonstrated to be very versatile and fulfil with the requirements for their use in PNR.Open access funding provided by FCT|FCCN (b-on).SpringerUniversidade do MinhoEscobar Fernandez, AneCarvalho, Mariana R.Silva, Tiago H.Reis, R. L.Oliveira, Joaquim M.2023-042023-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/85736engEscobar A., Carvalho M. R., Silva T. H., Reis R. L., Oliveira J. M. Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration, In vitro models, pp. 1-11, doi:10.1007/s44164-023-00050-3, 20232731-344110.1007/s44164-023-00050-3http://dx.doi.org/10.1007/s44164-023-00050-3info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T04:50:56Zoai:repositorium.sdum.uminho.pt:1822/85736Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:00:14.725Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration
title Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration
spellingShingle Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration
Escobar Fernandez, Ane
Conduits
Peripheral Nerve Regeneration (PNR)
Silk Fibroin
NGCs
title_short Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration
title_full Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration
title_fullStr Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration
title_full_unstemmed Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration
title_sort Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration
author Escobar Fernandez, Ane
author_facet Escobar Fernandez, Ane
Carvalho, Mariana R.
Silva, Tiago H.
Reis, R. L.
Oliveira, Joaquim M.
author_role author
author2 Carvalho, Mariana R.
Silva, Tiago H.
Reis, R. L.
Oliveira, Joaquim M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Escobar Fernandez, Ane
Carvalho, Mariana R.
Silva, Tiago H.
Reis, R. L.
Oliveira, Joaquim M.
dc.subject.por.fl_str_mv Conduits
Peripheral Nerve Regeneration (PNR)
Silk Fibroin
NGCs
topic Conduits
Peripheral Nerve Regeneration (PNR)
Silk Fibroin
NGCs
description Published 18 April 2023
publishDate 2023
dc.date.none.fl_str_mv 2023-04
2023-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/85736
url https://hdl.handle.net/1822/85736
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Escobar A., Carvalho M. R., Silva T. H., Reis R. L., Oliveira J. M. Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration, In vitro models, pp. 1-11, doi:10.1007/s44164-023-00050-3, 2023
2731-3441
10.1007/s44164-023-00050-3
http://dx.doi.org/10.1007/s44164-023-00050-3
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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