Dengue virus capsid protein interacts specifically with very low-density lipoproteins

Bibliographic Details
Main Author: Faustino, André F.
Publication Date: 2014
Other Authors: Carvalho, Filomena A., Martins, Ivo C., Castanho, Miguel A. R. B., Mohana-Borges, Ronaldo, Almeida, Fábio C. L., Da Poian, Andrea T., Santos, Nuno C.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/10709
Summary: © 2014 Elsevier Inc. All rights reserved.
id RCAP_02b910a25a1ac11ec08b22634c212d04
oai_identifier_str oai:repositorio.ulisboa.pt:10451/10709
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling Dengue virus capsid protein interacts specifically with very low-density lipoproteinsDengue virus capsid proteinLipoproteinsSingle-molecule studiesAFM-based force spectroscopyDynamic light scattering© 2014 Elsevier Inc. All rights reserved.Dengue affects millions of people worldwide. No specific treatment is currently available, in part due to an incomplete understanding of the viral components' interactions with host cellular structures. We tested dengue virus (DENV) capsid protein (C) interaction with low- and very low-density lipoproteins (LDL and VLDL, respectively) using atomic force microscopy-based force spectroscopy, dynamic light scattering, NMR and computational analysis. Data reveal a specific DENV C interaction with VLDL, but not LDL. This binding is potassium-dependent and involves the DENV C N-terminal region, as previously observed for the DENV C-lipid droplets (LDs) interaction. A successful inhibition of DENV C-VLDL binding was achieved with a peptide drug lead. The similarities between LDs and VLDL, and between perilipin 3 (DENV C target on LDs) and ApoE, indicate ApoE as the molecular target on VLDL. We hypothesize that DENV may form lipoviroparticles, which would constitute a novel step on DENV life cycle.This work was supported by Fundação para a Ciência e Tecnologia – Ministério da Educação e Ciência (FCT-MEC, Portugal, projects PTDC/QUI-BIQ/112929/2009 and PTDC/SAU-ENB/117013/2010), Calouste Gulenkian Foundation (Portugal), European Union FP7-IRSES project MEMPEPACROSS, FCT-CAPES Portugal-Brazil joint cooperation projects, and by the Brazilian Funding Agencies CNPq, FAPERJ, FINEP, CAPES (PVE171/2012) and INCT-Dengue. AFF and ICM acknowledge FCT-MEC for fellowships SFRH/BD/77609/2011 and SFRH/BPD/74287/2010, respectively.ElsevierRepositório da Universidade de LisboaFaustino, André F.Carvalho, Filomena A.Martins, Ivo C.Castanho, Miguel A. R. B.Mohana-Borges, RonaldoAlmeida, Fábio C. L.Da Poian, Andrea T.Santos, Nuno C.2014-03-10T12:41:01Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10709engNanomedicine: Nanotechnology, Biology, and Medicine 10 (2014) 247–2551549-9634http://dx.doi.org/10.1016/j.nano.2013.06.004metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:06:42Zoai:repositorio.ulisboa.pt:10451/10709Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:35:09.670439Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Dengue virus capsid protein interacts specifically with very low-density lipoproteins
title Dengue virus capsid protein interacts specifically with very low-density lipoproteins
spellingShingle Dengue virus capsid protein interacts specifically with very low-density lipoproteins
Faustino, André F.
Dengue virus capsid protein
Lipoproteins
Single-molecule studies
AFM-based force spectroscopy
Dynamic light scattering
title_short Dengue virus capsid protein interacts specifically with very low-density lipoproteins
title_full Dengue virus capsid protein interacts specifically with very low-density lipoproteins
title_fullStr Dengue virus capsid protein interacts specifically with very low-density lipoproteins
title_full_unstemmed Dengue virus capsid protein interacts specifically with very low-density lipoproteins
title_sort Dengue virus capsid protein interacts specifically with very low-density lipoproteins
author Faustino, André F.
author_facet Faustino, André F.
Carvalho, Filomena A.
Martins, Ivo C.
Castanho, Miguel A. R. B.
Mohana-Borges, Ronaldo
Almeida, Fábio C. L.
Da Poian, Andrea T.
Santos, Nuno C.
author_role author
author2 Carvalho, Filomena A.
Martins, Ivo C.
Castanho, Miguel A. R. B.
Mohana-Borges, Ronaldo
Almeida, Fábio C. L.
Da Poian, Andrea T.
Santos, Nuno C.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Faustino, André F.
Carvalho, Filomena A.
Martins, Ivo C.
Castanho, Miguel A. R. B.
Mohana-Borges, Ronaldo
Almeida, Fábio C. L.
Da Poian, Andrea T.
Santos, Nuno C.
dc.subject.por.fl_str_mv Dengue virus capsid protein
Lipoproteins
Single-molecule studies
AFM-based force spectroscopy
Dynamic light scattering
topic Dengue virus capsid protein
Lipoproteins
Single-molecule studies
AFM-based force spectroscopy
Dynamic light scattering
description © 2014 Elsevier Inc. All rights reserved.
publishDate 2014
dc.date.none.fl_str_mv 2014-03-10T12:41:01Z
2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10709
url http://hdl.handle.net/10451/10709
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nanomedicine: Nanotechnology, Biology, and Medicine 10 (2014) 247–255
1549-9634
http://dx.doi.org/10.1016/j.nano.2013.06.004
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833601420351242240

Similar Items