Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats

Bibliographic Details
Main Author: Farias Junior, Paulo Mecenas Alves
Publication Date: 2017
Other Authors: Teixeira, Francisco Bruno, Fagundes, Nathalia Carolina Fernandes, Miranda, Giza Hellen Nonato, Bittencourt, Leonardo Oliveira, Paraense, Ricardo Sousa de Oliveira, Silva, Márcia Cristina Freitas, Sagica, Fernanda do Espirito Santo, Oliveira, Edivaldo Herculano Correa de, Crespo-Lopez, Maria Elena, Lima, Rafael Rodrigues
Format: Article
Language: eng
Source: Repositório Digital do Instituto Evandro Chagas (Patuá)
Download full: https://patua.iec.gov.br/handle/iec/2924
Summary: Methylmercury (MeHg) is one of the most toxic species of mercury, causing several systemic damages; however, its effect on the salivary glands has rarely been explored to date. This study was aimed at analyzing the mercury deposit, oxidative stress markers, and cell viability in parotid and submandibular rat salivary glands after chronic methylmercury intoxication. Herein, forty male Wistar rats (40 days old) were used in the experiment. The animals of the experimental group were intoxicated by intragastric gavage with MeHg at a dose of 0.04 mg per kg body weight per day for 35 days, whereas the control group received only corn oil, a diluent. After the period of intoxication, the glands were obtained for evaluation of total mercury deposit, cell viability, and the malondialdehyde (MDA) and the nitrite levels. Our results indicated mercury deposits in salivary glands, with a decrease in cell viability, higher levels of MDA in both glands of intoxicated animals, and a higher concentration of nitrite only in the submandibular gland of the mercury group. Thus, the intoxication by MeHg was able to generate deposits and oxidative stress in salivary glands that resulted in a decrease in cell viability in both types of glands.
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spelling Farias Junior, Paulo Mecenas AlvesTeixeira, Francisco BrunoFagundes, Nathalia Carolina FernandesMiranda, Giza Hellen NonatoBittencourt, Leonardo OliveiraParaense, Ricardo Sousa de OliveiraSilva, Márcia Cristina FreitasSagica, Fernanda do Espirito SantoOliveira, Edivaldo Herculano Correa deCrespo-Lopez, Maria ElenaLima, Rafael Rodrigues2018-02-06T16:16:48Z2018-02-06T16:16:48Z2017FARIAS JUNIOR, Paulo Mecenas Alves et al. Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats. Metallomics, v. 9, n. 12, p. 1778-1785, Dec. 2017. DOI: https://doi.org/10.1039/c7mt00168a1756-5901https://patua.iec.gov.br/handle/iec/292410.1039/c7mt00168aMethylmercury (MeHg) is one of the most toxic species of mercury, causing several systemic damages; however, its effect on the salivary glands has rarely been explored to date. This study was aimed at analyzing the mercury deposit, oxidative stress markers, and cell viability in parotid and submandibular rat salivary glands after chronic methylmercury intoxication. Herein, forty male Wistar rats (40 days old) were used in the experiment. The animals of the experimental group were intoxicated by intragastric gavage with MeHg at a dose of 0.04 mg per kg body weight per day for 35 days, whereas the control group received only corn oil, a diluent. After the period of intoxication, the glands were obtained for evaluation of total mercury deposit, cell viability, and the malondialdehyde (MDA) and the nitrite levels. Our results indicated mercury deposits in salivary glands, with a decrease in cell viability, higher levels of MDA in both glands of intoxicated animals, and a higher concentration of nitrite only in the submandibular gland of the mercury group. Thus, the intoxication by MeHg was able to generate deposits and oxidative stress in salivary glands that resulted in a decrease in cell viability in both types of glands.Federal University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Molecular Pharmacology. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil / Federal University of Pará. Institute of Natural Sciences. College of Natural Sciences. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Molecular Pharmacology. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil.engRoyal Society of ChemistryChronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMercúrioIntoxicação por MercúrioCompostos de Metilmercúrio / toxicidadeGlândulas Salivares / efeitos de drogasRatos Wistarinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/66e80511-6fca-4d32-bfe5-68b0ee910979/download52f1732ea66fbd1123abe39f5373b797MD52TEXTChronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats.pdf.txtChronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats.pdf.txtExtracted texttext/plain38151https://patua.iec.gov.br/bitstreams/7e668ae9-3669-48b5-a432-c45406287197/download25cc6003c42787f5e0da41d53bcbed88MD57THUMBNAILChronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats.pdf.jpgChronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats.pdf.jpgGenerated Thumbnailimage/jpeg6043https://patua.iec.gov.br/bitstreams/71163551-1df0-40db-b97c-0d8c38d7aae8/download41602cc4f29d79402b3337f0d70bd0ebMD58ORIGINALChronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats.pdfChronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats.pdfapplication/pdf2019651https://patua.iec.gov.br/bitstreams/054009bf-92a9-49c1-8cd3-e548c7bba8e6/downloadf8bbdd25fcfde7b3f4035f1d4a4eb8cfMD56iec/29242023-06-13 14:12:24.687oai:patua.iec.gov.br:iec/2924https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-06-13T14:12:24Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats
title Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats
spellingShingle Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats
Farias Junior, Paulo Mecenas Alves
Mercúrio
Intoxicação por Mercúrio
Compostos de Metilmercúrio / toxicidade
Glândulas Salivares / efeitos de drogas
Ratos Wistar
title_short Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats
title_full Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats
title_fullStr Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats
title_full_unstemmed Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats
title_sort Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats
author Farias Junior, Paulo Mecenas Alves
author_facet Farias Junior, Paulo Mecenas Alves
Teixeira, Francisco Bruno
Fagundes, Nathalia Carolina Fernandes
Miranda, Giza Hellen Nonato
Bittencourt, Leonardo Oliveira
Paraense, Ricardo Sousa de Oliveira
Silva, Márcia Cristina Freitas
Sagica, Fernanda do Espirito Santo
Oliveira, Edivaldo Herculano Correa de
Crespo-Lopez, Maria Elena
Lima, Rafael Rodrigues
author_role author
author2 Teixeira, Francisco Bruno
Fagundes, Nathalia Carolina Fernandes
Miranda, Giza Hellen Nonato
Bittencourt, Leonardo Oliveira
Paraense, Ricardo Sousa de Oliveira
Silva, Márcia Cristina Freitas
Sagica, Fernanda do Espirito Santo
Oliveira, Edivaldo Herculano Correa de
Crespo-Lopez, Maria Elena
Lima, Rafael Rodrigues
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Farias Junior, Paulo Mecenas Alves
Teixeira, Francisco Bruno
Fagundes, Nathalia Carolina Fernandes
Miranda, Giza Hellen Nonato
Bittencourt, Leonardo Oliveira
Paraense, Ricardo Sousa de Oliveira
Silva, Márcia Cristina Freitas
Sagica, Fernanda do Espirito Santo
Oliveira, Edivaldo Herculano Correa de
Crespo-Lopez, Maria Elena
Lima, Rafael Rodrigues
dc.subject.decsPrimary.pt_BR.fl_str_mv Mercúrio
Intoxicação por Mercúrio
Compostos de Metilmercúrio / toxicidade
Glândulas Salivares / efeitos de drogas
Ratos Wistar
topic Mercúrio
Intoxicação por Mercúrio
Compostos de Metilmercúrio / toxicidade
Glândulas Salivares / efeitos de drogas
Ratos Wistar
description Methylmercury (MeHg) is one of the most toxic species of mercury, causing several systemic damages; however, its effect on the salivary glands has rarely been explored to date. This study was aimed at analyzing the mercury deposit, oxidative stress markers, and cell viability in parotid and submandibular rat salivary glands after chronic methylmercury intoxication. Herein, forty male Wistar rats (40 days old) were used in the experiment. The animals of the experimental group were intoxicated by intragastric gavage with MeHg at a dose of 0.04 mg per kg body weight per day for 35 days, whereas the control group received only corn oil, a diluent. After the period of intoxication, the glands were obtained for evaluation of total mercury deposit, cell viability, and the malondialdehyde (MDA) and the nitrite levels. Our results indicated mercury deposits in salivary glands, with a decrease in cell viability, higher levels of MDA in both glands of intoxicated animals, and a higher concentration of nitrite only in the submandibular gland of the mercury group. Thus, the intoxication by MeHg was able to generate deposits and oxidative stress in salivary glands that resulted in a decrease in cell viability in both types of glands.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-06T16:16:48Z
dc.date.available.fl_str_mv 2018-02-06T16:16:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIAS JUNIOR, Paulo Mecenas Alves et al. Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats. Metallomics, v. 9, n. 12, p. 1778-1785, Dec. 2017. DOI: https://doi.org/10.1039/c7mt00168a
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2924
dc.identifier.issn.-.fl_str_mv 1756-5901
dc.identifier.doi.-.fl_str_mv 10.1039/c7mt00168a
identifier_str_mv FARIAS JUNIOR, Paulo Mecenas Alves et al. Chronic intoxication by methylmercury leads to oxidative damage and cell death in salivary glands of rats. Metallomics, v. 9, n. 12, p. 1778-1785, Dec. 2017. DOI: https://doi.org/10.1039/c7mt00168a
1756-5901
10.1039/c7mt00168a
url https://patua.iec.gov.br/handle/iec/2924
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
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instname_str Instituto Evandro Chagas (IEC)
instacron_str IEC
institution IEC
reponame_str Repositório Digital do Instituto Evandro Chagas (Patuá)
collection Repositório Digital do Instituto Evandro Chagas (Patuá)
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