Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats

Detalhes bibliográficos
Autor(a) principal: Rodrigues,Daniela
Data de Publicação: 2012
Outros Autores: Silveira,Themis Reverbel Da, Matte,Ursula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos de gastroenterologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032012000400011
Resumo: CONTEXT: Hepatocyte transplantation is an attractive therapeutic modality for liver disease as an alternative for orthotopic liver transplantation. OBJECTIVE: The aim of the current study was to investigate the feasibility of freshly isolated rat hepatocyte transplantation in acetaminophen-induced hepatotoxicity model. METHODS: Hepatocytes were isolated from male Wistar rats and transplanted 24 hours after acetaminophen administration in female recipients. Female rats received either 1x10(7) hepatocytes or phosphate buffered saline through the portal vein or into the spleen and were sacrificed after 48 hours. RESULTS: Alanine aminotransferase levels measured within the experiment did not differ between groups at any time point. Molecular analysis and histology showed presence of hepatocytes in liver of transplanted animals injected either through portal vein or spleen. CONCLUSION: These data demonstrate the feasibility and efficacy of hepatocyte transplantation in the liver or spleen in a mild acetaminophen-induced hepatotoxicity model.
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spelling Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in ratsHepatocytes, transplantationAcetaminophenDrug-induced liver injuryRatsCONTEXT: Hepatocyte transplantation is an attractive therapeutic modality for liver disease as an alternative for orthotopic liver transplantation. OBJECTIVE: The aim of the current study was to investigate the feasibility of freshly isolated rat hepatocyte transplantation in acetaminophen-induced hepatotoxicity model. METHODS: Hepatocytes were isolated from male Wistar rats and transplanted 24 hours after acetaminophen administration in female recipients. Female rats received either 1x10(7) hepatocytes or phosphate buffered saline through the portal vein or into the spleen and were sacrificed after 48 hours. RESULTS: Alanine aminotransferase levels measured within the experiment did not differ between groups at any time point. Molecular analysis and histology showed presence of hepatocytes in liver of transplanted animals injected either through portal vein or spleen. CONCLUSION: These data demonstrate the feasibility and efficacy of hepatocyte transplantation in the liver or spleen in a mild acetaminophen-induced hepatotoxicity model.Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE. 2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032012000400011Arquivos de Gastroenterologia v.49 n.4 2012reponame:Arquivos de gastroenterologia (Online)instname:Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologiainstacron:IBEPEGE10.1590/S0004-28032012000400011info:eu-repo/semantics/openAccessRodrigues,DanielaSilveira,Themis Reverbel DaMatte,Ursulaeng2013-09-11T00:00:00Zoai:scielo:S0004-28032012000400011Revistahttp://www.scielo.br/aghttps://old.scielo.br/oai/scielo-oai.php||secretariaarqgastr@hospitaligesp.com.br1678-42190004-2803opendoar:2013-09-11T00:00Arquivos de gastroenterologia (Online) - Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologiafalse
dc.title.none.fl_str_mv Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats
title Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats
spellingShingle Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats
Rodrigues,Daniela
Hepatocytes, transplantation
Acetaminophen
Drug-induced liver injury
Rats
title_short Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats
title_full Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats
title_fullStr Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats
title_full_unstemmed Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats
title_sort Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats
author Rodrigues,Daniela
author_facet Rodrigues,Daniela
Silveira,Themis Reverbel Da
Matte,Ursula
author_role author
author2 Silveira,Themis Reverbel Da
Matte,Ursula
author2_role author
author
dc.contributor.author.fl_str_mv Rodrigues,Daniela
Silveira,Themis Reverbel Da
Matte,Ursula
dc.subject.por.fl_str_mv Hepatocytes, transplantation
Acetaminophen
Drug-induced liver injury
Rats
topic Hepatocytes, transplantation
Acetaminophen
Drug-induced liver injury
Rats
description CONTEXT: Hepatocyte transplantation is an attractive therapeutic modality for liver disease as an alternative for orthotopic liver transplantation. OBJECTIVE: The aim of the current study was to investigate the feasibility of freshly isolated rat hepatocyte transplantation in acetaminophen-induced hepatotoxicity model. METHODS: Hepatocytes were isolated from male Wistar rats and transplanted 24 hours after acetaminophen administration in female recipients. Female rats received either 1x10(7) hepatocytes or phosphate buffered saline through the portal vein or into the spleen and were sacrificed after 48 hours. RESULTS: Alanine aminotransferase levels measured within the experiment did not differ between groups at any time point. Molecular analysis and histology showed presence of hepatocytes in liver of transplanted animals injected either through portal vein or spleen. CONCLUSION: These data demonstrate the feasibility and efficacy of hepatocyte transplantation in the liver or spleen in a mild acetaminophen-induced hepatotoxicity model.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032012000400011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032012000400011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0004-28032012000400011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE.
publisher.none.fl_str_mv Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE.
dc.source.none.fl_str_mv Arquivos de Gastroenterologia v.49 n.4 2012
reponame:Arquivos de gastroenterologia (Online)
instname:Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia
instacron:IBEPEGE
instname_str Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia
instacron_str IBEPEGE
institution IBEPEGE
reponame_str Arquivos de gastroenterologia (Online)
collection Arquivos de gastroenterologia (Online)
repository.name.fl_str_mv Arquivos de gastroenterologia (Online) - Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia
repository.mail.fl_str_mv ||secretariaarqgastr@hospitaligesp.com.br
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