Rabies virus in McCoy cell line: Part II – Titration
| Main Author: | |
|---|---|
| Publication Date: | 1992 |
| Other Authors: | |
| Format: | Article |
| Language: | por |
| Source: | Revista do Instituto Adolfo Lutz (Online) |
| Download full: | https://periodicos.saude.sp.gov.br/RIAL/article/view/35959 |
Summary: | The McCoy cell line has showed high sensivity to rabies virus. This cell line presents cythopatic effect (CPE), when infected with rabies virus. Three strains of rabies virus were tested: CVS, ERA, and PV1 and three street virus were isolated: one from a patient bitten by bat, other from a rabid dog and another from bovine. This cell line was used for virus titration purposes due to the presence of cytophatic effect, two techniques for observation of the CPE were used and were compared to the classical method of intracerebral inoculation in mice. |
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Revista do Instituto Adolfo Lutz (Online) |
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Rabies virus in McCoy cell line: Part II – TitrationVirus da raiva em linhagem celular McCoy: Parte II - TitulaçãoMcCoy cell lineRabies Virus(CVS, ERA, PVl and street virus).Isolationtitration and cytopathic effect (CPE)Linhagem celular McCoyVírus da raiva(CVS, ERA, PVI e vírus de rua). Isolamentotitulação e efeito citopático (ECP)The McCoy cell line has showed high sensivity to rabies virus. This cell line presents cythopatic effect (CPE), when infected with rabies virus. Three strains of rabies virus were tested: CVS, ERA, and PV1 and three street virus were isolated: one from a patient bitten by bat, other from a rabid dog and another from bovine. This cell line was used for virus titration purposes due to the presence of cytophatic effect, two techniques for observation of the CPE were used and were compared to the classical method of intracerebral inoculation in mice.A linhagem celular McCoy tem demonstrado elevada sensibilidade ao vírus da raiva. Essa linhagem apresenta efeito citopático (ECP) quando infectada com o vírus rábico. Foram testadas três cepas de vírus da raiva: CVS, ERA e PVI e foram isolados três vírus de rua: um proveniente de paciente mordido por morcego, outro de origem canina e outro de origem bovina. Utilizamos essa linhagem celular também para fins de titulação de vírus. Dada a presença do efeito citopático, foram empregadas duas técnicas de observação do ECP e ambas foram comparadas ao método clássico de inoculação em cérebros de camundongos.Instituto Adolfo Lutz1992-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/RIAL/article/view/3595910.53393/rial.1992.52.35959Instituto Adolfo Lutz Journal - RIAL; Vol. 52 No. 1-2 (1992); 17-21Revista del Instituto Adolfo Lutz - RIAL; Vol. 52 Núm. 1-2 (1992); 17-21Revista do Instituto Adolfo Lutz; v. 52 n. 1-2 (1992); 17-211983-38140073-9855reponame:Revista do Instituto Adolfo Lutz (Online)instname:Instituto Adolfo Lutzinstacron:IALporhttps://periodicos.saude.sp.gov.br/RIAL/article/view/35959/34298Copyright (c) 1992 Yeda L. Nogueira, Claudia F. C. do Amaralinfo:eu-repo/semantics/openAccessNogueira, Yeda L. Amaral, Claudia F. C. do 2024-02-21T18:35:01Zoai:ojs.periodicos.saude.sp.gov.br:article/35959Revistahttps://periodicos.saude.sp.gov.br/RIAL/indexPUBhttps://periodicos.saude.sp.gov.br/RIAL/oairial@saude.sp.gov.brhttps://doi.org/10.53393/rial1983-38140073-9855opendoar:2024-02-21T18:35:01Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutzfalse |
| dc.title.none.fl_str_mv |
Rabies virus in McCoy cell line: Part II – Titration Virus da raiva em linhagem celular McCoy: Parte II - Titulação |
| title |
Rabies virus in McCoy cell line: Part II – Titration |
| spellingShingle |
Rabies virus in McCoy cell line: Part II – Titration Nogueira, Yeda L. McCoy cell line Rabies Virus (CVS, ERA, PVl and street virus).Isolation titration and cytopathic effect (CPE) Linhagem celular McCoy Vírus da raiva (CVS, ERA, PVI e vírus de rua). Isolamento titulação e efeito citopático (ECP) |
| title_short |
Rabies virus in McCoy cell line: Part II – Titration |
| title_full |
Rabies virus in McCoy cell line: Part II – Titration |
| title_fullStr |
Rabies virus in McCoy cell line: Part II – Titration |
| title_full_unstemmed |
Rabies virus in McCoy cell line: Part II – Titration |
| title_sort |
Rabies virus in McCoy cell line: Part II – Titration |
| author |
Nogueira, Yeda L. |
| author_facet |
Nogueira, Yeda L. Amaral, Claudia F. C. do |
| author_role |
author |
| author2 |
Amaral, Claudia F. C. do |
| author2_role |
author |
| dc.contributor.author.fl_str_mv |
Nogueira, Yeda L. Amaral, Claudia F. C. do |
| dc.subject.por.fl_str_mv |
McCoy cell line Rabies Virus (CVS, ERA, PVl and street virus).Isolation titration and cytopathic effect (CPE) Linhagem celular McCoy Vírus da raiva (CVS, ERA, PVI e vírus de rua). Isolamento titulação e efeito citopático (ECP) |
| topic |
McCoy cell line Rabies Virus (CVS, ERA, PVl and street virus).Isolation titration and cytopathic effect (CPE) Linhagem celular McCoy Vírus da raiva (CVS, ERA, PVI e vírus de rua). Isolamento titulação e efeito citopático (ECP) |
| description |
The McCoy cell line has showed high sensivity to rabies virus. This cell line presents cythopatic effect (CPE), when infected with rabies virus. Three strains of rabies virus were tested: CVS, ERA, and PV1 and three street virus were isolated: one from a patient bitten by bat, other from a rabid dog and another from bovine. This cell line was used for virus titration purposes due to the presence of cytophatic effect, two techniques for observation of the CPE were used and were compared to the classical method of intracerebral inoculation in mice. |
| publishDate |
1992 |
| dc.date.none.fl_str_mv |
1992-12-30 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado pelos pares |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://periodicos.saude.sp.gov.br/RIAL/article/view/35959 10.53393/rial.1992.52.35959 |
| url |
https://periodicos.saude.sp.gov.br/RIAL/article/view/35959 |
| identifier_str_mv |
10.53393/rial.1992.52.35959 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.none.fl_str_mv |
https://periodicos.saude.sp.gov.br/RIAL/article/view/35959/34298 |
| dc.rights.driver.fl_str_mv |
Copyright (c) 1992 Yeda L. Nogueira, Claudia F. C. do Amaral info:eu-repo/semantics/openAccess |
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Copyright (c) 1992 Yeda L. Nogueira, Claudia F. C. do Amaral |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Instituto Adolfo Lutz |
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Instituto Adolfo Lutz |
| dc.source.none.fl_str_mv |
Instituto Adolfo Lutz Journal - RIAL; Vol. 52 No. 1-2 (1992); 17-21 Revista del Instituto Adolfo Lutz - RIAL; Vol. 52 Núm. 1-2 (1992); 17-21 Revista do Instituto Adolfo Lutz; v. 52 n. 1-2 (1992); 17-21 1983-3814 0073-9855 reponame:Revista do Instituto Adolfo Lutz (Online) instname:Instituto Adolfo Lutz instacron:IAL |
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Instituto Adolfo Lutz |
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IAL |
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IAL |
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Revista do Instituto Adolfo Lutz (Online) |
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Revista do Instituto Adolfo Lutz (Online) |
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Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutz |
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rial@saude.sp.gov.br |
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1836991963291713536 |