Ochratoxin A in raisins samples marketed in São Paulo, Brazil

Bibliographic Details
Main Author: Almeida, Adriana Palma de
Publication Date: 2006
Other Authors: Alaburda, Janete, Ruvieri, Valter, Sabino, Myrna
Format: Article
Language: eng
Source: Revista do Instituto Adolfo Lutz (Online)
Download full: https://periodicos.saude.sp.gov.br/RIAL/article/view/32860
Summary: The present study aimed to evaluate a methodology for analyzing ochratoxin A (OTA) in raisins samples marketed in São Paulo, Brazil. OTA was extracted with methanol:water (80:20, v/v) and diluted with phosphate buffer solution, and purified through an immunoaffinity column. OTA was separated and quantified using HPLC with fluorescence detection. The established detection and quantification limits were 0.24 and 0.80 ng/g, respectively. The recoveries values were 81.6, 80.4 and 81.9%, and the relative standard deviations (RSD) were 6.0, 4.3, and 6.1 % at 2.0, 5.0 and 10.0 ng/g levels, respectively. Of twenty black raisin samples analyzed 10 (50 %) contained OTA at levels ranging from 1.3 to 39.1 ng/g. All of twenty-two white raisin samples showed no contamination with OTA.
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spelling Ochratoxin A in raisins samples marketed in São Paulo, BrazilOcratoxina A em amostras de uva passa comercializadas em São Paulo, Brasilochratoxin AOTAraisinsHPLCimmunoaffinity columnocratoxina AOTAuva passaCLAEcoluna de imunoafinidadeThe present study aimed to evaluate a methodology for analyzing ochratoxin A (OTA) in raisins samples marketed in São Paulo, Brazil. OTA was extracted with methanol:water (80:20, v/v) and diluted with phosphate buffer solution, and purified through an immunoaffinity column. OTA was separated and quantified using HPLC with fluorescence detection. The established detection and quantification limits were 0.24 and 0.80 ng/g, respectively. The recoveries values were 81.6, 80.4 and 81.9%, and the relative standard deviations (RSD) were 6.0, 4.3, and 6.1 % at 2.0, 5.0 and 10.0 ng/g levels, respectively. Of twenty black raisin samples analyzed 10 (50 %) contained OTA at levels ranging from 1.3 to 39.1 ng/g. All of twenty-two white raisin samples showed no contamination with OTA.O presente estudo teve como objetivo avaliar uma metodologia para análise de ocratoxina A (OTA) em amostras de uva passa comercializadas em São Paulo, Brasil. A OTA foi extraída com metanol: água(80:20, v/v) e diluída com solução tampão fosfato, utilizando-se coluna de imunoafinidade para purificação da amostra. A OTA foi separada e quantificada por meio de CLAE com detector de fluorescência. Os limites de detecção e de quantificação estabelecidos foram respectivamente de 0,24 e 0,80 ng/g. Os valores de recuperação foram 81,6; 80,4 e 81,9% e os coeficientes de variação foram 6,0; 4,3 e 6,1 % respectivamente para os níveis de 2,0; 5,0 e 10,0 ng/g. De um total de vinte amostras de uvas passas pretas, 10 (50 %) continham OTA na faixa de concentrações de 1,3 a 39,1 ng/g. Todas as vinte e duas amostras de uvas passas brancas não apresentaram contaminação por OTA.Instituto Adolfo Lutz2006-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/RIAL/article/view/3286010.53393/rial.2006.65.32860Instituto Adolfo Lutz Journal - RIAL; Vol. 65 No. 3 (2006); 171-175Revista del Instituto Adolfo Lutz - RIAL; Vol. 65 Núm. 3 (2006); 171-175Revista do Instituto Adolfo Lutz; v. 65 n. 3 (2006); 171-1751983-38140073-9855reponame:Revista do Instituto Adolfo Lutz (Online)instname:Instituto Adolfo Lutzinstacron:IALenghttps://periodicos.saude.sp.gov.br/RIAL/article/view/32860/31691Copyright (c) 2006 Adriana Palma de Almeida, Janete Alaburda, Valter Ruvieri, Myrna Sabinoinfo:eu-repo/semantics/openAccessAlmeida, Adriana Palma deAlaburda, JaneteRuvieri, ValterSabino, Myrna2024-01-22T19:46:30Zoai:ojs.periodicos.saude.sp.gov.br:article/32860Revistahttps://periodicos.saude.sp.gov.br/RIAL/indexPUBhttps://periodicos.saude.sp.gov.br/RIAL/oairial@saude.sp.gov.brhttps://doi.org/10.53393/rial1983-38140073-9855opendoar:2024-01-22T19:46:30Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutzfalse
dc.title.none.fl_str_mv Ochratoxin A in raisins samples marketed in São Paulo, Brazil
Ocratoxina A em amostras de uva passa comercializadas em São Paulo, Brasil
title Ochratoxin A in raisins samples marketed in São Paulo, Brazil
spellingShingle Ochratoxin A in raisins samples marketed in São Paulo, Brazil
Almeida, Adriana Palma de
ochratoxin A
OTA
raisins
HPLC
immunoaffinity column
ocratoxina A
OTA
uva passa
CLAE
coluna de imunoafinidade
title_short Ochratoxin A in raisins samples marketed in São Paulo, Brazil
title_full Ochratoxin A in raisins samples marketed in São Paulo, Brazil
title_fullStr Ochratoxin A in raisins samples marketed in São Paulo, Brazil
title_full_unstemmed Ochratoxin A in raisins samples marketed in São Paulo, Brazil
title_sort Ochratoxin A in raisins samples marketed in São Paulo, Brazil
author Almeida, Adriana Palma de
author_facet Almeida, Adriana Palma de
Alaburda, Janete
Ruvieri, Valter
Sabino, Myrna
author_role author
author2 Alaburda, Janete
Ruvieri, Valter
Sabino, Myrna
author2_role author
author
author
dc.contributor.author.fl_str_mv Almeida, Adriana Palma de
Alaburda, Janete
Ruvieri, Valter
Sabino, Myrna
dc.subject.por.fl_str_mv ochratoxin A
OTA
raisins
HPLC
immunoaffinity column
ocratoxina A
OTA
uva passa
CLAE
coluna de imunoafinidade
topic ochratoxin A
OTA
raisins
HPLC
immunoaffinity column
ocratoxina A
OTA
uva passa
CLAE
coluna de imunoafinidade
description The present study aimed to evaluate a methodology for analyzing ochratoxin A (OTA) in raisins samples marketed in São Paulo, Brazil. OTA was extracted with methanol:water (80:20, v/v) and diluted with phosphate buffer solution, and purified through an immunoaffinity column. OTA was separated and quantified using HPLC with fluorescence detection. The established detection and quantification limits were 0.24 and 0.80 ng/g, respectively. The recoveries values were 81.6, 80.4 and 81.9%, and the relative standard deviations (RSD) were 6.0, 4.3, and 6.1 % at 2.0, 5.0 and 10.0 ng/g levels, respectively. Of twenty black raisin samples analyzed 10 (50 %) contained OTA at levels ranging from 1.3 to 39.1 ng/g. All of twenty-two white raisin samples showed no contamination with OTA.
publishDate 2006
dc.date.none.fl_str_mv 2006-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/32860
10.53393/rial.2006.65.32860
url https://periodicos.saude.sp.gov.br/RIAL/article/view/32860
identifier_str_mv 10.53393/rial.2006.65.32860
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/32860/31691
dc.rights.driver.fl_str_mv Copyright (c) 2006 Adriana Palma de Almeida, Janete Alaburda, Valter Ruvieri, Myrna Sabino
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2006 Adriana Palma de Almeida, Janete Alaburda, Valter Ruvieri, Myrna Sabino
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Adolfo Lutz
publisher.none.fl_str_mv Instituto Adolfo Lutz
dc.source.none.fl_str_mv Instituto Adolfo Lutz Journal - RIAL; Vol. 65 No. 3 (2006); 171-175
Revista del Instituto Adolfo Lutz - RIAL; Vol. 65 Núm. 3 (2006); 171-175
Revista do Instituto Adolfo Lutz; v. 65 n. 3 (2006); 171-175
1983-3814
0073-9855
reponame:Revista do Instituto Adolfo Lutz (Online)
instname:Instituto Adolfo Lutz
instacron:IAL
instname_str Instituto Adolfo Lutz
instacron_str IAL
institution IAL
reponame_str Revista do Instituto Adolfo Lutz (Online)
collection Revista do Instituto Adolfo Lutz (Online)
repository.name.fl_str_mv Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutz
repository.mail.fl_str_mv rial@saude.sp.gov.br
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