Comparison of laboratorial tests for the diagnosis of human T-lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected patients

Bibliographic Details
Main Author: Campos, Karoline Rodrigues
Publication Date: 2015
Other Authors: Gonçalves, Maria Gisele, Fukasawa, Lucila Okuyama, Costa, Nadia Aparecida, Barreto-Damião, Carlos Henrique, Magri, Mariana Cavalheiro, Alencar, Wong Kuen, Caterino-De-Araujo, Adele
Format: Article
Language: por
Source: Revista do Instituto Adolfo Lutz (Online)
Download full: https://periodicos.saude.sp.gov.br/RIAL/article/view/33386
Summary: The present study aimed at investigating the best algorithm definition to be employed for diagnosing the human T lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected/Aids patients. Blood samples of 1,608 HIV-1 infected patients from the AIDS Reference Center (CRT DST/AIDS-SP) were tested for the presence of HTLV-1/2 antibodies using two screening assays (EIA Murex HTLV-I+II, and Gold ELISA HTLV-I/II), and confirmed by two Blots [HTLV Blot 2.4 (Western Blot –WB) and INNO-LIA HTLV I/II (Line ImmunoAssay - LIA)], and one molecular assay (pol real-time PCR). The screening tests detected 51(Murex) and 49 (Gold ELISA) reagents sera. WB , confirmed 23 HTLV-1, 12 HTLV-2, six HTLV, and nine showed HTLV indeterminate profiles. LIA confirmed 24 HTLV-1, 20 HTLV-2, and six HTLV. PCR detected 18 HTLV-1- and 12 HTLV-2-infected blood samples. By using any confirmatory assay, 50 patients confirmed HTLV infection: 25 HTLV-1 (1.55 %), 21 HTLV-2 (1.31 %) and four HTLV (0.25 %). The sensitivity of LIA, WB and PCR assays were 96 %, 76 % and 60 %, respectively. By considering the assays cost as the sole variable, the best testing algorithm for diagnosing HIV-1/HTLV-coinfection in HIV-1 infected patient was the use of PCR followed by LIA technique.
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spelling Comparison of laboratorial tests for the diagnosis of human T-lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected patientsComparação de testes laboratoriais para o diagnóstico de infecção por vírus linfotrópicos de células T humanas do tipo 1 (HTLV-1) e tipo 2 (HTLV-2) em pacientes infectados por HIV-1HTLV-1/2diagnosisserologyPCRHIV-1/HTLV-coinfectionalgorithm testsHTLV-1/2diagnósticosorologiaPCRcoinfecção HIV-1/HTLValgoritmo de testes laboratoriaisThe present study aimed at investigating the best algorithm definition to be employed for diagnosing the human T lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected/Aids patients. Blood samples of 1,608 HIV-1 infected patients from the AIDS Reference Center (CRT DST/AIDS-SP) were tested for the presence of HTLV-1/2 antibodies using two screening assays (EIA Murex HTLV-I+II, and Gold ELISA HTLV-I/II), and confirmed by two Blots [HTLV Blot 2.4 (Western Blot –WB) and INNO-LIA HTLV I/II (Line ImmunoAssay - LIA)], and one molecular assay (pol real-time PCR). The screening tests detected 51(Murex) and 49 (Gold ELISA) reagents sera. WB , confirmed 23 HTLV-1, 12 HTLV-2, six HTLV, and nine showed HTLV indeterminate profiles. LIA confirmed 24 HTLV-1, 20 HTLV-2, and six HTLV. PCR detected 18 HTLV-1- and 12 HTLV-2-infected blood samples. By using any confirmatory assay, 50 patients confirmed HTLV infection: 25 HTLV-1 (1.55 %), 21 HTLV-2 (1.31 %) and four HTLV (0.25 %). The sensitivity of LIA, WB and PCR assays were 96 %, 76 % and 60 %, respectively. By considering the assays cost as the sole variable, the best testing algorithm for diagnosing HIV-1/HTLV-coinfection in HIV-1 infected patient was the use of PCR followed by LIA technique.O presente estudo pesquisou o melhor algoritmo de testes laboratoriais para efetuar o diagnóstico de infecção por vírus linfotrópicos de células T humanas dos tipos 1 (HTLV-1) e 2 (HTLV-2) em pacientes HIV-1 positivos. Amostras de sangue de 1.608 pacientes do CRT DST/Aids-SP foram analisadas quanto à presença de anticorpos específicos usando-se dois ensaios de triagem (EIA Murex HTLV-I+II e Gold ELISA HTLV-I/II), dois confirmatórios [HTLV Blot 2.4 (Western Blot – WB) e INNO-LIA HTLV I/II (Line ImmunoAssay - LIA)] e um molecular (PCR em tempo real pol). Na triagem foram detectados 51(Murex) e 49 (Gold ELISA) soros reagentes. Pelo WB, 23 soros confirmaram infecção por HTLV-1, 12 HTLV-2, seis HTLV e nove apresentaram perfis indeterminados. O LIA detectou 24 soros HTLV-1 positivos, 20 HTLV-2 e seis HTLV. A PCR evidenciou segmento pol de HTLV-1 em 18 e HTLV-2 em 12 amostras de sangue. Pelos testes confirmatórios, em 50 pacientes foi confirmada a infecção por HTLV: 25 HTLV-1 (1,55 %), 21 HTLV-2 (1,31 %) e quatro HTLV (0,25 %). As sensibilidades do LIA, WB e PCR foram de 96 %, 76 % e 60 %, respectivamente. Considerando-se apenas o custo, o melhor algoritmo diagnóstico para população infectada pelo HIV-1 foi o uso da PCR seguida do LIA.Instituto Adolfo Lutz2015-10-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/RIAL/article/view/3338610.53393/rial.2015.v74.33386Instituto Adolfo Lutz Journal - RIAL; Vol. 74 No. 1 (2015); 57-65Revista del Instituto Adolfo Lutz - RIAL; Vol. 74 Núm. 1 (2015); 57-65Revista do Instituto Adolfo Lutz; v. 74 n. 1 (2015); 57-651983-38140073-9855reponame:Revista do Instituto Adolfo Lutz (Online)instname:Instituto Adolfo Lutzinstacron:IALporhttps://periodicos.saude.sp.gov.br/RIAL/article/view/33386/32218Copyright (c) 2015 Karoline Rodrigues Campos, Maria Gisele Gonçalves, Lucila Okuyama Fukasawa, Nadia Aparecida Costa, Carlos Henrique Barreto-Damião, Mariana Cavalheiro Magri, Wong Kuen Alencar, Adele Caterino-De-Araujoinfo:eu-repo/semantics/openAccessCampos, Karoline RodriguesGonçalves, Maria GiseleFukasawa, Lucila OkuyamaCosta, Nadia AparecidaBarreto-Damião, Carlos HenriqueMagri, Mariana CavalheiroAlencar, Wong KuenCaterino-De-Araujo, Adele2023-11-07T19:38:52Zoai:ojs.periodicos.saude.sp.gov.br:article/33386Revistahttps://periodicos.saude.sp.gov.br/RIAL/indexPUBhttps://periodicos.saude.sp.gov.br/RIAL/oairial@saude.sp.gov.brhttps://doi.org/10.53393/rial1983-38140073-9855opendoar:2023-11-07T19:38:52Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutzfalse
dc.title.none.fl_str_mv Comparison of laboratorial tests for the diagnosis of human T-lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected patients
Comparação de testes laboratoriais para o diagnóstico de infecção por vírus linfotrópicos de células T humanas do tipo 1 (HTLV-1) e tipo 2 (HTLV-2) em pacientes infectados por HIV-1
title Comparison of laboratorial tests for the diagnosis of human T-lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected patients
spellingShingle Comparison of laboratorial tests for the diagnosis of human T-lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected patients
Campos, Karoline Rodrigues
HTLV-1/2
diagnosis
serology
PCR
HIV-1/HTLV-coinfection
algorithm tests
HTLV-1/2
diagnóstico
sorologia
PCR
coinfecção HIV-1/HTLV
algoritmo de testes laboratoriais
title_short Comparison of laboratorial tests for the diagnosis of human T-lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected patients
title_full Comparison of laboratorial tests for the diagnosis of human T-lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected patients
title_fullStr Comparison of laboratorial tests for the diagnosis of human T-lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected patients
title_full_unstemmed Comparison of laboratorial tests for the diagnosis of human T-lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected patients
title_sort Comparison of laboratorial tests for the diagnosis of human T-lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected patients
author Campos, Karoline Rodrigues
author_facet Campos, Karoline Rodrigues
Gonçalves, Maria Gisele
Fukasawa, Lucila Okuyama
Costa, Nadia Aparecida
Barreto-Damião, Carlos Henrique
Magri, Mariana Cavalheiro
Alencar, Wong Kuen
Caterino-De-Araujo, Adele
author_role author
author2 Gonçalves, Maria Gisele
Fukasawa, Lucila Okuyama
Costa, Nadia Aparecida
Barreto-Damião, Carlos Henrique
Magri, Mariana Cavalheiro
Alencar, Wong Kuen
Caterino-De-Araujo, Adele
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Campos, Karoline Rodrigues
Gonçalves, Maria Gisele
Fukasawa, Lucila Okuyama
Costa, Nadia Aparecida
Barreto-Damião, Carlos Henrique
Magri, Mariana Cavalheiro
Alencar, Wong Kuen
Caterino-De-Araujo, Adele
dc.subject.por.fl_str_mv HTLV-1/2
diagnosis
serology
PCR
HIV-1/HTLV-coinfection
algorithm tests
HTLV-1/2
diagnóstico
sorologia
PCR
coinfecção HIV-1/HTLV
algoritmo de testes laboratoriais
topic HTLV-1/2
diagnosis
serology
PCR
HIV-1/HTLV-coinfection
algorithm tests
HTLV-1/2
diagnóstico
sorologia
PCR
coinfecção HIV-1/HTLV
algoritmo de testes laboratoriais
description The present study aimed at investigating the best algorithm definition to be employed for diagnosing the human T lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) in HIV-1 infected/Aids patients. Blood samples of 1,608 HIV-1 infected patients from the AIDS Reference Center (CRT DST/AIDS-SP) were tested for the presence of HTLV-1/2 antibodies using two screening assays (EIA Murex HTLV-I+II, and Gold ELISA HTLV-I/II), and confirmed by two Blots [HTLV Blot 2.4 (Western Blot –WB) and INNO-LIA HTLV I/II (Line ImmunoAssay - LIA)], and one molecular assay (pol real-time PCR). The screening tests detected 51(Murex) and 49 (Gold ELISA) reagents sera. WB , confirmed 23 HTLV-1, 12 HTLV-2, six HTLV, and nine showed HTLV indeterminate profiles. LIA confirmed 24 HTLV-1, 20 HTLV-2, and six HTLV. PCR detected 18 HTLV-1- and 12 HTLV-2-infected blood samples. By using any confirmatory assay, 50 patients confirmed HTLV infection: 25 HTLV-1 (1.55 %), 21 HTLV-2 (1.31 %) and four HTLV (0.25 %). The sensitivity of LIA, WB and PCR assays were 96 %, 76 % and 60 %, respectively. By considering the assays cost as the sole variable, the best testing algorithm for diagnosing HIV-1/HTLV-coinfection in HIV-1 infected patient was the use of PCR followed by LIA technique.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-05
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/33386
10.53393/rial.2015.v74.33386
url https://periodicos.saude.sp.gov.br/RIAL/article/view/33386
identifier_str_mv 10.53393/rial.2015.v74.33386
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/33386/32218
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Adolfo Lutz
publisher.none.fl_str_mv Instituto Adolfo Lutz
dc.source.none.fl_str_mv Instituto Adolfo Lutz Journal - RIAL; Vol. 74 No. 1 (2015); 57-65
Revista del Instituto Adolfo Lutz - RIAL; Vol. 74 Núm. 1 (2015); 57-65
Revista do Instituto Adolfo Lutz; v. 74 n. 1 (2015); 57-65
1983-3814
0073-9855
reponame:Revista do Instituto Adolfo Lutz (Online)
instname:Instituto Adolfo Lutz
instacron:IAL
instname_str Instituto Adolfo Lutz
instacron_str IAL
institution IAL
reponame_str Revista do Instituto Adolfo Lutz (Online)
collection Revista do Instituto Adolfo Lutz (Online)
repository.name.fl_str_mv Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutz
repository.mail.fl_str_mv rial@saude.sp.gov.br
_version_ 1836991960964923392

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