Isolation and characterization of endophytic bacteria of coffee plants and their potential in caffeine degradation.

• Autor(a) principal: NUNES, F. V.
• Data de Publicação: 2006
• Outros Autores: MELO, I. S. de
• Idioma: por
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1026079
• topic: Coffee; Endophytic microorganisms; Caffeine; Biodegradation

The plant kingdom is colonized by a diverse array of endophytic bacteria, which form a non-pathogenic relationship with their hosts. Endophytic bacteria may confer benefits to the plant, and the benefits may be reciprocal, resulting in an enhanced symbiotic system for specifíc plant characteristics. The objective of this study was to isolate endophytic bacteria from coffee {Coffea arabica and C. robusta) and evaluate their potential in degrading caffeine. The isolates were identified by partial 16S rDNA sequence analysis and by FAME and the analysis of biodegradation of caffeine was carried out by HPLC. As a result, 252 bacteria! strains were isolated, with most of them belonging to the species Bacillus lentimorbus, B. meguterium, B. subtilis, B. cereus, Pseudomonas putida, P. chlororaphis, Pantoea ananatis, P. agglomerans, Stenotrophomonas malthophilia, Kluyvera cryocrescens, Kocuria kristinae etc. Approximately 20% of the bacterial strains showed the ability to grow in the presence of high concentrations (5.000 mg.L-1) of caffeine and two Pseudomonas putida strains completely degraded the alkaloid, showing the potential ofendophytic bacteria in decaffeination processes.