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Disinfection and in vitro establishment of Acacia mearnsii nodal segments

Bibliographic Details
Main Author: Salles, Ecléia Alexandra Poltronieri Buda
Publication Date: 2017
Other Authors: Alcantara, Giovana Bomfim, Quoirin, Marguerite Germaine Ghislaine, Gonçalves, Antonio Natal, Higa, Antonio Rioyei
Format: Article
Language: por
Source: Pesquisa Florestal Brasileira (Online)
Download full: https://pfb.sede.embrapa.br/pfb/article/view/1392
Summary: Due to low rates of rooting it is difficult to propagate selected clones of Acacia mearnsii by cuttings. The purpose of this study was to define a protocol for disinfection of nodal segments for in vitro introduction, aiming to improve the species genetically for forest plantation. Combination of different products concentration (70% ethyl alcohol, sodium hypochlorite, plant preservative mixture and mercuric chloride), immersion period and size of nodal segment were tested. The best disinfection treatment was the combination of 70% ethanol, 0.4% mercuric chloride, sodium hypochlorite at 2% in nodal segments of 10 mm, with 80% survival.
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spelling Disinfection and in vitro establishment of Acacia mearnsii nodal segmentsDesinfestação e introdução in vitro de segmentos nodais de Acacia mearnsiiAcacia negraClonesEstaquiaBlack wattleClonesCuttingDue to low rates of rooting it is difficult to propagate selected clones of Acacia mearnsii by cuttings. The purpose of this study was to define a protocol for disinfection of nodal segments for in vitro introduction, aiming to improve the species genetically for forest plantation. Combination of different products concentration (70% ethyl alcohol, sodium hypochlorite, plant preservative mixture and mercuric chloride), immersion period and size of nodal segment were tested. The best disinfection treatment was the combination of 70% ethanol, 0.4% mercuric chloride, sodium hypochlorite at 2% in nodal segments of 10 mm, with 80% survival.A dificuldade em produzir clones de Acacia mearnsii por estaquia, em função das baixas taxas de enraizamento, constitui um empecilho para o uso desta técnica. O objetivo deste estudo foi o desenvolvimento de um protocolo de desinfestação para introdução in vitro de segmentos nodais de clones selecionados de A. mearnsii, visando aprimorar o melhoramento genético da espécie para plantios florestais. A desinfestação de propágulos, provenientes de jardim clonal, foi realizada combinando diferentes produtos e concentrações (álcool etílico 70%, hipoclorito de sódio, plant preservative mixture e cloreto de mercúrio), tempo de imersão e tamanho dos segmentos nodais (4 mm e 10 mm). Segmentos nodais de 10 mm responderam melhor ao tratamento de desinfestação, com 80% de sobrevivência, combinando álcool etílico 70%, cloreto de mercúrio a 0,4% e hipoclorito de sódio a 2%.Embrapa Florestas2017-12-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed articleArtigo avaliado pelos paresapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttps://pfb.sede.embrapa.br/pfb/article/view/139210.4336/2017.pfb.37.92.1392Pesquisa Florestal Brasileira; Vol. 37 No. 92 (2017): out./dez.; 485-491Pesquisa Florestal Brasileira; v. 37 n. 92 (2017): out./dez.; 485-4911983-26051809-3647reponame:Pesquisa Florestal Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://pfb.sede.embrapa.br/pfb/article/view/1392/607https://pfb.sede.embrapa.br/pfb/article/view/1392/1378https://pfb.sede.embrapa.br/pfb/article/view/1392/1379Salles, Ecléia Alexandra Poltronieri BudaAlcantara, Giovana BomfimQuoirin, Marguerite Germaine GhislaineGonçalves, Antonio NatalHiga, Antonio Rioyeiinfo:eu-repo/semantics/openAccess2019-01-02T18:39:23Zoai:ojs.pkp.sfu.ca:article/1392Revistahttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/PUBhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/oaipfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br1983-26051809-3647opendoar:2019-01-02T18:39:23Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Disinfection and in vitro establishment of Acacia mearnsii nodal segments
Desinfestação e introdução in vitro de segmentos nodais de Acacia mearnsii
title Disinfection and in vitro establishment of Acacia mearnsii nodal segments
spellingShingle Disinfection and in vitro establishment of Acacia mearnsii nodal segments
Salles, Ecléia Alexandra Poltronieri Buda
Acacia negra
Clones
Estaquia
Black wattle
Clones
Cutting
title_short Disinfection and in vitro establishment of Acacia mearnsii nodal segments
title_full Disinfection and in vitro establishment of Acacia mearnsii nodal segments
title_fullStr Disinfection and in vitro establishment of Acacia mearnsii nodal segments
title_full_unstemmed Disinfection and in vitro establishment of Acacia mearnsii nodal segments
title_sort Disinfection and in vitro establishment of Acacia mearnsii nodal segments
author Salles, Ecléia Alexandra Poltronieri Buda
author_facet Salles, Ecléia Alexandra Poltronieri Buda
Alcantara, Giovana Bomfim
Quoirin, Marguerite Germaine Ghislaine
Gonçalves, Antonio Natal
Higa, Antonio Rioyei
author_role author
author2 Alcantara, Giovana Bomfim
Quoirin, Marguerite Germaine Ghislaine
Gonçalves, Antonio Natal
Higa, Antonio Rioyei
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Salles, Ecléia Alexandra Poltronieri Buda
Alcantara, Giovana Bomfim
Quoirin, Marguerite Germaine Ghislaine
Gonçalves, Antonio Natal
Higa, Antonio Rioyei
dc.subject.por.fl_str_mv Acacia negra
Clones
Estaquia
Black wattle
Clones
Cutting
topic Acacia negra
Clones
Estaquia
Black wattle
Clones
Cutting
description Due to low rates of rooting it is difficult to propagate selected clones of Acacia mearnsii by cuttings. The purpose of this study was to define a protocol for disinfection of nodal segments for in vitro introduction, aiming to improve the species genetically for forest plantation. Combination of different products concentration (70% ethyl alcohol, sodium hypochlorite, plant preservative mixture and mercuric chloride), immersion period and size of nodal segment were tested. The best disinfection treatment was the combination of 70% ethanol, 0.4% mercuric chloride, sodium hypochlorite at 2% in nodal segments of 10 mm, with 80% survival.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed article
Artigo avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://pfb.sede.embrapa.br/pfb/article/view/1392
10.4336/2017.pfb.37.92.1392
url https://pfb.sede.embrapa.br/pfb/article/view/1392
identifier_str_mv 10.4336/2017.pfb.37.92.1392
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://pfb.sede.embrapa.br/pfb/article/view/1392/607
https://pfb.sede.embrapa.br/pfb/article/view/1392/1378
https://pfb.sede.embrapa.br/pfb/article/view/1392/1379
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/vnd.openxmlformats-officedocument.wordprocessingml.document
dc.publisher.none.fl_str_mv Embrapa Florestas
publisher.none.fl_str_mv Embrapa Florestas
dc.source.none.fl_str_mv Pesquisa Florestal Brasileira; Vol. 37 No. 92 (2017): out./dez.; 485-491
Pesquisa Florestal Brasileira; v. 37 n. 92 (2017): out./dez.; 485-491
1983-2605
1809-3647
reponame:Pesquisa Florestal Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Florestal Brasileira (Online)
collection Pesquisa Florestal Brasileira (Online)
repository.name.fl_str_mv Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br
_version_ 1831476824149852160

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