Export Ready — 

In vitro propagation and structural analysis of apple tree

Bibliographic Details
Main Author: Rodrigues, Michele de Medeiros
Publication Date: 2006
Other Authors: Melo, Maria das Dores, Aloufa, Magdi Ahmed Ibrahim
Format: Article
Language: por
Source: Pesquisa Agropecuária Brasileira (Online)
Download full: https://seer.sct.embrapa.br/index.php/pab/article/view/7126
Summary: Effects of different concentrations of saccharose on multiplicative rates of in vitro sprouts and on anatomy of vegetative organs of young apple plants were tested. The micropropagation was obtained by direct organogenesis starting from stem apexes and internodal segments. Explants were removed from young plants, germinated in vitro at two months age. For the anatomical analysis of vegetative organs, plantlets developped in M&S medium after 30 days of culture were used. Results showed that the medium without sucrose caused death of the explant. The highest rate of shoots appeared using explants of stem apexes, and the size of the shoots in the concentrations 30, 45 and 60 g L-1 varied between 1 and 3 cm. The leaf presents structural alterations according to the medium sucrose concentration.
id EMBRAPA-4_312b4f4736f1a97f662e35e92ccf15ba
oai_identifier_str oai:ojs.seer.sct.embrapa.br:article/7126
network_acronym_str EMBRAPA-4
network_name_str Pesquisa Agropecuária Brasileira (Online)
repository_id_str
spelling In vitro propagation and structural analysis of apple treePropagação vegetativa in vitro e análise estrutural de macieiraMalus domestica; in vitro culture; anatomyMalus domestica; cultura in vitro; anatomiaEffects of different concentrations of saccharose on multiplicative rates of in vitro sprouts and on anatomy of vegetative organs of young apple plants were tested. The micropropagation was obtained by direct organogenesis starting from stem apexes and internodal segments. Explants were removed from young plants, germinated in vitro at two months age. For the anatomical analysis of vegetative organs, plantlets developped in M&S medium after 30 days of culture were used. Results showed that the medium without sucrose caused death of the explant. The highest rate of shoots appeared using explants of stem apexes, and the size of the shoots in the concentrations 30, 45 and 60 g L-1 varied between 1 and 3 cm. The leaf presents structural alterations according to the medium sucrose concentration.Foram testados os efeitos de diferentes concentrações de sacarose sobre a taxa multiplicativa de brotos in vitro e sobre a anatomia dos órgãos vegetativos de plântulas de macieira. A micropropagação foi obtida por organogênese direta a partir de ápices caulinares e segmentos internodais. Na análise estrutural, utilizou-se plântula desenvolvida em meio MS após 30 dias de cultura. A ausência de sacarose no meio de cultura ocasionou a morte ou atrofiamento do explante. A maior taxa de brotos foi obtida com os explantes de ápices caulinares, e o tamanho médio dos brotos, nas concentrações 30, 45 e 60 g L-1, variou entre 1 e 3 cm. A folha apresentou alterações estruturais de acordo com as concentrações de sacarose do meio.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraRodrigues, Michele de MedeirosMelo, Maria das DoresAloufa, Magdi Ahmed Ibrahim2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7126Pesquisa Agropecuaria Brasileira; v.41, n.1, jan. 2006; 171-173Pesquisa Agropecuária Brasileira; v.41, n.1, jan. 2006; 171-1731678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7126/4171info:eu-repo/semantics/openAccess2014-09-25T17:09:25Zoai:ojs.seer.sct.embrapa.br:article/7126Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-25T17:09:25Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv In vitro propagation and structural analysis of apple tree
Propagação vegetativa in vitro e análise estrutural de macieira
title In vitro propagation and structural analysis of apple tree
spellingShingle In vitro propagation and structural analysis of apple tree
Rodrigues, Michele de Medeiros
Malus domestica; in vitro culture; anatomy
Malus domestica; cultura in vitro; anatomia
title_short In vitro propagation and structural analysis of apple tree
title_full In vitro propagation and structural analysis of apple tree
title_fullStr In vitro propagation and structural analysis of apple tree
title_full_unstemmed In vitro propagation and structural analysis of apple tree
title_sort In vitro propagation and structural analysis of apple tree
author Rodrigues, Michele de Medeiros
author_facet Rodrigues, Michele de Medeiros
Melo, Maria das Dores
Aloufa, Magdi Ahmed Ibrahim
author_role author
author2 Melo, Maria das Dores
Aloufa, Magdi Ahmed Ibrahim
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Rodrigues, Michele de Medeiros
Melo, Maria das Dores
Aloufa, Magdi Ahmed Ibrahim
dc.subject.por.fl_str_mv Malus domestica; in vitro culture; anatomy
Malus domestica; cultura in vitro; anatomia
topic Malus domestica; in vitro culture; anatomy
Malus domestica; cultura in vitro; anatomia
description Effects of different concentrations of saccharose on multiplicative rates of in vitro sprouts and on anatomy of vegetative organs of young apple plants were tested. The micropropagation was obtained by direct organogenesis starting from stem apexes and internodal segments. Explants were removed from young plants, germinated in vitro at two months age. For the anatomical analysis of vegetative organs, plantlets developped in M&S medium after 30 days of culture were used. Results showed that the medium without sucrose caused death of the explant. The highest rate of shoots appeared using explants of stem apexes, and the size of the shoots in the concentrations 30, 45 and 60 g L-1 varied between 1 and 3 cm. The leaf presents structural alterations according to the medium sucrose concentration.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7126
url https://seer.sct.embrapa.br/index.php/pab/article/view/7126
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7126/4171
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.41, n.1, jan. 2006; 171-173
Pesquisa Agropecuária Brasileira; v.41, n.1, jan. 2006; 171-173
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
_version_ 1830213313685880832

Similar Items