Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
| Main Author: | |
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| Publication Date: | 2012 |
| Other Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1128/JVI.06796-11 |
| Download full: | https://arca.fiocruz.br/handle/icict/23019 |
Summary: | Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal. |
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Carvalho, Filomena A.Carneiro, Fabiana A.Martins, Ivo C.Miranda, Iranaia AssunçãoFaustino, André F.Pereira, Renata M.Bozza, Patrícia T.Castanho, Miguel A. R. B.Borges, Ronaldo Mohanada Poian, Andrea T.Santos, Nuno C.2017-11-01T13:15:02Z2017-11-01T13:15:02Z2012CARVALHO, Filomena A. et al. Dengue Virus Capsid Protein Binding to Hepatic Lipid Droplets (LD) Is Potassium Ion Dependent and Is Mediated by LD Surface Proteins. Journal of Virology, v.86, n.4, p.2096-2108, Feb. 2012.0022-538Xhttps://arca.fiocruz.br/handle/icict/2301910.1128/JVI.06796-111098-5514engAmerican Society for MicrobiologyVírus da DengueProteínas de MembranaPotássioLípido hepáticoDengue virusHepatic Lipid DropletsLD Surface ProteinsPotassiumDengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Pólo Avançado de Xerém. Duque de Caxias, RJ, Brasil.Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.Universidade Federal do Rio de Janeiro, Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.Universidade Federal do Rio de Janeiro, Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.Dengue virus (DENV) affects millions of people, causing more than 20,000 deaths annually. No effective treatment for the disease caused by DENV infection is currently available, partially due to the lack of knowledge on the basic aspects of the viral life cycle, including the molecular basis of the interaction between viral components and cellular compartments. Here, we characterized the properties of the interaction between the DENV capsid (C) protein and hepatic lipid droplets (LDs), which was recently shown to be essential for the virus replication cycle. Zeta potential analysis revealed a negative surface charge of LDs, with an average surface charge of -19 mV. The titration of LDs with C protein led to an increase of the surface charge, which reached a plateau at +13.7 mV, suggesting that the viral protein-LD interaction exposes the protein cationic surface to the aqueous environment. Atomic force microscopy (AFM)-based force spectroscopy measurements were performed by using C protein-functionalized AFM tips. The C protein-LD interaction was found to be strong, with a single (un)binding force of 33.6 pN. This binding was dependent on high intracellular concentrations of potassium ions but not sodium. The inhibition of Na(+)/K(+)-ATPase in DENV-infected cells resulted in the dissociation of C protein from LDs and a 50-fold inhibition of infectious virus production but not of RNA replication, indicating a biological relevance for the potassium-dependent interaction. Limited proteolysis of the LD surface impaired the C protein-LD interaction, and force measurements in the presence of specific antibodies indicated that perilipin 3 (TIP47) is the major DENV C protein ligand on the surface of LDs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/628c1501-2ea0-4ccf-bcb8-00d9215100ee/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALpatricia2_bozza_etal_IOC_2012.pdfpatricia2_bozza_etal_IOC_2012.pdfapplication/pdf2219358https://arca.fiocruz.br/bitstreams/f54af165-1eb2-411d-8948-a7f33d77932d/downloadd2e446a37fd88dd259d30d4e8f256da4MD52trueAnonymousREADTEXTpatricia2_bozza_etal_IOC_2012.pdf.txtpatricia2_bozza_etal_IOC_2012.pdf.txtExtracted texttext/plain75443https://arca.fiocruz.br/bitstreams/3a17f05a-49f3-4b6c-9b3e-122613a455c8/downloadd57fd536d1f03d5b544a3464fa343d63MD55falseAnonymousREADTHUMBNAILpatricia2_bozza_etal_IOC_2012.pdf.jpgpatricia2_bozza_etal_IOC_2012.pdf.jpgGenerated Thumbnailimage/jpeg6112https://arca.fiocruz.br/bitstreams/7f407ca9-d6e6-4ec4-b90a-3269b0a0f34b/download921bf267c088ca7d4d8a1449bee57267MD56falseAnonymousREADicict/230192025-07-30 00:04:12.53open.accessoai:arca.fiocruz.br:icict/23019https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T03:04:12Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.none.fl_str_mv |
Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins |
| title |
Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins |
| spellingShingle |
Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins Carvalho, Filomena A. Vírus da Dengue Proteínas de Membrana Potássio Lípido hepático Dengue virus Hepatic Lipid Droplets LD Surface Proteins Potassium |
| title_short |
Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins |
| title_full |
Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins |
| title_fullStr |
Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins |
| title_full_unstemmed |
Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins |
| title_sort |
Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins |
| author |
Carvalho, Filomena A. |
| author_facet |
Carvalho, Filomena A. Carneiro, Fabiana A. Martins, Ivo C. Miranda, Iranaia Assunção Faustino, André F. Pereira, Renata M. Bozza, Patrícia T. Castanho, Miguel A. R. B. Borges, Ronaldo Mohana da Poian, Andrea T. Santos, Nuno C. |
| author_role |
author |
| author2 |
Carneiro, Fabiana A. Martins, Ivo C. Miranda, Iranaia Assunção Faustino, André F. Pereira, Renata M. Bozza, Patrícia T. Castanho, Miguel A. R. B. Borges, Ronaldo Mohana da Poian, Andrea T. Santos, Nuno C. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Carvalho, Filomena A. Carneiro, Fabiana A. Martins, Ivo C. Miranda, Iranaia Assunção Faustino, André F. Pereira, Renata M. Bozza, Patrícia T. Castanho, Miguel A. R. B. Borges, Ronaldo Mohana da Poian, Andrea T. Santos, Nuno C. |
| dc.subject.other.none.fl_str_mv |
Vírus da Dengue Proteínas de Membrana Potássio Lípido hepático |
| topic |
Vírus da Dengue Proteínas de Membrana Potássio Lípido hepático Dengue virus Hepatic Lipid Droplets LD Surface Proteins Potassium |
| dc.subject.en.none.fl_str_mv |
Dengue virus Hepatic Lipid Droplets LD Surface Proteins Potassium |
| description |
Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal. |
| publishDate |
2012 |
| dc.date.issued.fl_str_mv |
2012 |
| dc.date.accessioned.fl_str_mv |
2017-11-01T13:15:02Z |
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2017-11-01T13:15:02Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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CARVALHO, Filomena A. et al. Dengue Virus Capsid Protein Binding to Hepatic Lipid Droplets (LD) Is Potassium Ion Dependent and Is Mediated by LD Surface Proteins. Journal of Virology, v.86, n.4, p.2096-2108, Feb. 2012. |
| dc.identifier.uri.fl_str_mv |
https://arca.fiocruz.br/handle/icict/23019 |
| dc.identifier.issn.none.fl_str_mv |
0022-538X |
| dc.identifier.doi.none.fl_str_mv |
10.1128/JVI.06796-11 |
| dc.identifier.eissn.none.fl_str_mv |
1098-5514 |
| identifier_str_mv |
CARVALHO, Filomena A. et al. Dengue Virus Capsid Protein Binding to Hepatic Lipid Droplets (LD) Is Potassium Ion Dependent and Is Mediated by LD Surface Proteins. Journal of Virology, v.86, n.4, p.2096-2108, Feb. 2012. 0022-538X 10.1128/JVI.06796-11 1098-5514 |
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https://arca.fiocruz.br/handle/icict/23019 |
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eng |
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eng |
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openAccess |
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American Society for Microbiology |
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American Society for Microbiology |
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