Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits
| Main Author: | |
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| Publication Date: | 2024 |
| Other Authors: | , , , , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1038/s41598-024-52364-1 |
| Download full: | https://arca.fiocruz.br/handle/icict/66927 |
Summary: | Supplementary Information The online version contains supplementary material available at https://doi.org/10.1038/s41598-024-52364-1. |
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Penteado, Renato FerrasSilva, Renata SantanaMoura, Danielle Maria NascimentoLima, Gustavo BarbosaMalvezzi, Amaranta MunizMonteiro, Tallyta Tâmara da SilvaXavier, Camila CavalcantiVichier‑guerre, SophieDugué, LaurencePochet, SylvieZanchin, Nilson Ivo ToninReis, Christian Robson de SouzaMelo Neto, Osvaldo Pompilio deGuimarães, Beatriz Gomes2024-11-04T20:46:25Z2024-11-04T20:46:25Z2024PENTEADO, R. F. et al. Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits. Scientific Reports, v. 14, n. 2178, p. 1-18, 2024.2045-2322https://arca.fiocruz.br/handle/icict/6692710.1038/s41598-024-52364-1Supplementary Information The online version contains supplementary material available at https://doi.org/10.1038/s41598-024-52364-1.CNPq, Institut Pasteur Paris, Deutsche ForschungsgemeinschafFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Institut Pasteur. Epigenetic Chemical Biology. Paris, France.Institut Pasteur. Epigenetic Chemical Biology. Paris, France.Institut Pasteur. Epigenetic Chemical Biology. Paris, France.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Recognition of the mRNA 5′ end is a critical step needed for translation initiation. This step is performed by the cap binding protein eIF4E, which joins the larger eIF4G subunit to form the eIF4F complex. Trypanosomatids have a minimum of fve diferent eIF4F-like complexes formed through specifc but not well-defned interactions between four diferent eIF4E and fve eIF4G homologues. The EIF4E6/EIF4G5 complex has been linked with the stage-specifc translation of mRNAs encoding the major Trypanosoma brucei virulence factors. Here, to better defne the molecular basis for the TbEIF4E6/TbEIF4G5 interaction, we describe the identifcation of the peptide interacting with TbEIF4E6 in the region comprising residues 79–166 of TbEIF4G5. The TbEIF4E6-TbEIF4G5_79-116 complex reconstituted with recombinant proteins is highly stable even in the absence of cap-4. The crystal structure of the complex was subsequently solved, revealing extensive interacting surfaces. Comparative analyses highlight the conservation of the overall structural arrangement of diferent eIF4E/eIF4G complexes. However, highly diferent interacting surfaces are formed with distinct binding contacts occurring both in the canonical and noncanonical elements within eIF4G and the respective eIF4E counterpart. These specifc pairs of complementary interacting surfaces are likely responsible for the selective association needed for the formation of distinct eIF4F complexes in trypanosomatids.engNatureInteração peptídicaTrypanosomatídeomRNAProteína de ligação ao capTrypanosomatidsmRNAPeptide interactionCap binding proteinmRNATrypanosoma bruceiStructural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunitsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALve_Renato_Penteado_ICC_IAM_2024.pdfve_Renato_Penteado_ICC_IAM_2024.pdfapplication/pdf13110498https://arca.fiocruz.br/bitstreams/4d99b7fa-d4d9-4efb-b940-518f57d76f32/download19ba93d54fed8500f82354331ee9cd5aMD52trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/8260ed71-d0c2-4d3a-9600-57839218fd0f/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADTEXTve_Renato_Penteado_ICC_IAM_2024.pdf.txtve_Renato_Penteado_ICC_IAM_2024.pdf.txtExtracted texttext/plain77860https://arca.fiocruz.br/bitstreams/c1348e57-25f0-42ed-9dcc-4180f4c07575/downloadee1c961b4210450bbfba8526f75d380aMD55falseAnonymousREADTHUMBNAILve_Renato_Penteado_ICC_IAM_2024.pdf.jpgve_Renato_Penteado_ICC_IAM_2024.pdf.jpgGenerated Thumbnailimage/jpeg6362https://arca.fiocruz.br/bitstreams/00cbaf86-4829-4c8b-8dcd-d60cbd62137e/downloade4cd8e88c6794df88ce7f2a825e8a7f4MD56falseAnonymousREADicict/669272025-07-30 00:34:08.376open.accessoai:arca.fiocruz.br:icict/66927https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T03:34:08Repositório Institucional da FIOCRUZ (ARCA) - 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Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits |
| title |
Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits |
| spellingShingle |
Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits Penteado, Renato Ferras Interação peptídica Trypanosomatídeo mRNA Proteína de ligação ao cap Trypanosomatids mRNA Peptide interaction Cap binding protein mRNA Trypanosoma brucei |
| title_short |
Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits |
| title_full |
Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits |
| title_fullStr |
Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits |
| title_full_unstemmed |
Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits |
| title_sort |
Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits |
| author |
Penteado, Renato Ferras |
| author_facet |
Penteado, Renato Ferras Silva, Renata Santana Moura, Danielle Maria Nascimento Lima, Gustavo Barbosa Malvezzi, Amaranta Muniz Monteiro, Tallyta Tâmara da Silva Xavier, Camila Cavalcanti Vichier‑guerre, Sophie Dugué, Laurence Pochet, Sylvie Zanchin, Nilson Ivo Tonin Reis, Christian Robson de Souza Melo Neto, Osvaldo Pompilio de Guimarães, Beatriz Gomes |
| author_role |
author |
| author2 |
Silva, Renata Santana Moura, Danielle Maria Nascimento Lima, Gustavo Barbosa Malvezzi, Amaranta Muniz Monteiro, Tallyta Tâmara da Silva Xavier, Camila Cavalcanti Vichier‑guerre, Sophie Dugué, Laurence Pochet, Sylvie Zanchin, Nilson Ivo Tonin Reis, Christian Robson de Souza Melo Neto, Osvaldo Pompilio de Guimarães, Beatriz Gomes |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Penteado, Renato Ferras Silva, Renata Santana Moura, Danielle Maria Nascimento Lima, Gustavo Barbosa Malvezzi, Amaranta Muniz Monteiro, Tallyta Tâmara da Silva Xavier, Camila Cavalcanti Vichier‑guerre, Sophie Dugué, Laurence Pochet, Sylvie Zanchin, Nilson Ivo Tonin Reis, Christian Robson de Souza Melo Neto, Osvaldo Pompilio de Guimarães, Beatriz Gomes |
| dc.subject.other.none.fl_str_mv |
Interação peptídica Trypanosomatídeo mRNA Proteína de ligação ao cap |
| topic |
Interação peptídica Trypanosomatídeo mRNA Proteína de ligação ao cap Trypanosomatids mRNA Peptide interaction Cap binding protein mRNA Trypanosoma brucei |
| dc.subject.en.none.fl_str_mv |
Trypanosomatids mRNA Peptide interaction Cap binding protein |
| dc.subject.decs.none.fl_str_mv |
mRNA Trypanosoma brucei |
| description |
Supplementary Information The online version contains supplementary material available at https://doi.org/10.1038/s41598-024-52364-1. |
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2024 |
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2024-11-04T20:46:25Z |
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2024-11-04T20:46:25Z |
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2024 |
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info:eu-repo/semantics/article |
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PENTEADO, R. F. et al. Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits. Scientific Reports, v. 14, n. 2178, p. 1-18, 2024. |
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https://arca.fiocruz.br/handle/icict/66927 |
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2045-2322 |
| dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-024-52364-1 |
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PENTEADO, R. F. et al. Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits. Scientific Reports, v. 14, n. 2178, p. 1-18, 2024. 2045-2322 10.1038/s41598-024-52364-1 |
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openAccess |
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Nature |
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Nature |
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