Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient
| Main Author: | |
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| Publication Date: | 2024 |
| Other Authors: | , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1016/j.meegid.2024.105598 |
| Download full: | https://arca.fiocruz.br/handle/icict/64570 |
Summary: | Produção científica do Laboratório de Pesquisa em Infecção Hospitalar (vigente até jan/2023). |
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Boff, LuanaDuarte, Humberlânia de SousaKraychete, Gabriela BergianteRocha, Gabriel TaddeucciOliveira, Bianca DinizAlbano, Rodolpho MattosAssef, Ana Paula D'Alincourt CarvalhoSuperti, Silvana VargasMartins, Ianick SoutoPicão, Renata Cristina2024-06-19T17:52:39Z2024-06-19T17:52:39Z2024BOFF, Luana et al. Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient. Infection, Genetics and Evolution, v. 121, p. 1-4, July 2024.1567-1348https://arca.fiocruz.br/handle/icict/6457010.1016/j.meegid.2024.105598Produção científica do Laboratório de Pesquisa em Infecção Hospitalar (vigente até jan/2023).This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) [process numbers 311737/2019–6, 408725/2022–2].Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) [process numbers, E-26/200.795/2019, E-26/211.554/2019, E-26/211.163/2019, E-26/201.191/2021, E-26/211.351/2021].INPRA - Instituto Nacional de Pesquisa em Resistência Antimicrobiana - Brazil [INCT/CNPq: 465718/2014–0].Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil [CAPES, financing code 001].The corresponding author always had full access to study data and had final responsibility for the decision to submit for publication.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Laboratório de Investigação em Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Laboratório de Investigação em Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Laboratório de Investigação em Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Laboratório de Investigação em Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Hospital do Câncer I. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia Roberto Alcantara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Hospital do Câncer I. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Hospital do Câncer I. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Faculdade de Medicina. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Laboratório de Investigação em Microbiologia Médica. Rio de Janeiro, RJ, Brasil / Universidade Federal de São Carlos. Centro de Ciências Biológicas e da Saúde. Departamento de Hidrobiologia. São Carlos, SP, Brasil.Klebsiella pneumoniae is an opportunistic pathogen that can colonize the gastrointestinal tract (GIT) of humans. The mechanisms underlying the successful translocation of this pathogen to cause extra-intestinal infections remain unknown, although virulence and antimicrobial resistance traits likely play significant roles in the establishment of infections. We investigated K. pneumoniae strains isolated from GIT colonization (strains Kp_FZcol-1, Kp_FZcol-2 and Kp_FZcro-1) and from a fatal bloodstream infection (strain Kp_HM-1) in a leukemia patient. All strains belonged to ST307, carried a transferable IncF plasmid containing the blaCTX-M-15 gene (pKPN3–307 TypeA-like plasmid) and showed a multidrug-resistance phenotype. Phylogenetic analysis demonstrated that Kp_HM-1 was more closely related to Kp_FZcro-1 than to the other colonizing strains. The Kp_FZcol-2 genome showed 81 % coverage with the Kp_HM-1 246,730 bp plasmid (pKp_HM-1), lacking most of its putative virulence genes. Searching public genomes with similar coverage, we observed the occurrence of this deletion in K. pneumoniae ST307 strains recovered from human colonization and infection in different countries. Our findings suggest that strains lacking the putative virulence genes found in the pKPN3–307 TypeA plasmid are still able to colonize and infect humans, highlighting the need to further investigate the role of these genes for the adaptation of K. pneumoniae ST307 in distinct human body sites.engElsevierComparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patientinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleKlebsiella pneumoniaeST307Bacterial translocationVirulenceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/eb999bac-4375-4448-a506-fd04724ade17/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALve_Ana_Assef_etal_IOC_2024.pdfve_Ana_Assef_etal_IOC_2024.pdfapplication/pdf2346447https://arca.fiocruz.br/bitstreams/2741461f-b887-42a7-b4c9-b5462cc5f903/download6ee176d40e25f0436f7dff892589c48cMD52trueAnonymousREADTHUMBNAILcapa_Ana_Assef_etal_IOC_2024.jpgcapa_Ana_Assef_etal_IOC_2024.jpgCapaimage/jpeg2362535https://arca.fiocruz.br/bitstreams/71e04e7b-79d7-44de-808a-165c23fb0dd3/download9a8b70ae8e13f48782dcf5d035fbb5ccMD53falseAnonymousREADve_Ana_Assef_etal_IOC_2024.pdf.jpgve_Ana_Assef_etal_IOC_2024.pdf.jpgGenerated Thumbnailimage/jpeg5699https://arca.fiocruz.br/bitstreams/ab0dfa71-7735-449a-a0aa-c97f8b8d4676/download717c0c9f659b2e806166f41093a66e5eMD57falseAnonymousREADTEXTve_Ana_Assef_etal_IOC_2024.pdf.txtve_Ana_Assef_etal_IOC_2024.pdf.txtExtracted texttext/plain25718https://arca.fiocruz.br/bitstreams/1e9caf4b-26d6-40dc-ada7-d9bb8a77466c/download1d6c63d03a81cd7c014bdd20a1d403deMD56falseAnonymousREADicict/645702025-07-29 23:16:45.716open.accessoai:arca.fiocruz.br:icict/64570https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T02:16:45Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.none.fl_str_mv |
Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient |
| title |
Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient |
| spellingShingle |
Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient Boff, Luana Klebsiella pneumoniae ST307 Bacterial translocation Virulence |
| title_short |
Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient |
| title_full |
Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient |
| title_fullStr |
Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient |
| title_full_unstemmed |
Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient |
| title_sort |
Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient |
| author |
Boff, Luana |
| author_facet |
Boff, Luana Duarte, Humberlânia de Sousa Kraychete, Gabriela Bergiante Rocha, Gabriel Taddeucci Oliveira, Bianca Diniz Albano, Rodolpho Mattos Assef, Ana Paula D'Alincourt Carvalho Superti, Silvana Vargas Martins, Ianick Souto Picão, Renata Cristina |
| author_role |
author |
| author2 |
Duarte, Humberlânia de Sousa Kraychete, Gabriela Bergiante Rocha, Gabriel Taddeucci Oliveira, Bianca Diniz Albano, Rodolpho Mattos Assef, Ana Paula D'Alincourt Carvalho Superti, Silvana Vargas Martins, Ianick Souto Picão, Renata Cristina |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Boff, Luana Duarte, Humberlânia de Sousa Kraychete, Gabriela Bergiante Rocha, Gabriel Taddeucci Oliveira, Bianca Diniz Albano, Rodolpho Mattos Assef, Ana Paula D'Alincourt Carvalho Superti, Silvana Vargas Martins, Ianick Souto Picão, Renata Cristina |
| dc.subject.en.none.fl_str_mv |
Klebsiella pneumoniae ST307 Bacterial translocation Virulence |
| topic |
Klebsiella pneumoniae ST307 Bacterial translocation Virulence |
| description |
Produção científica do Laboratório de Pesquisa em Infecção Hospitalar (vigente até jan/2023). |
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2024 |
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2024-06-19T17:52:39Z |
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2024-06-19T17:52:39Z |
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2024 |
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info:eu-repo/semantics/article |
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BOFF, Luana et al. Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient. Infection, Genetics and Evolution, v. 121, p. 1-4, July 2024. |
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https://arca.fiocruz.br/handle/icict/64570 |
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1567-1348 |
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10.1016/j.meegid.2024.105598 |
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BOFF, Luana et al. Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient. Infection, Genetics and Evolution, v. 121, p. 1-4, July 2024. 1567-1348 10.1016/j.meegid.2024.105598 |
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Elsevier |
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Elsevier |
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