Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.

Bibliographic Details
Main Author: Santos, Isabela C. S.
Publication Date: 2024
Other Authors: Avelar, Daniel Moreira de, Miranda, Luciana F. C., Mello, Cintia X. de, Keidel, Lucas, Pimentel, Maria Inês F., Ventura, Luanna S., Fagundes, Aline, Santos, Fernanda N., Oliveira, Liliane F. A., Santos, Shanna A., Pereira, Sandro Antonio, Menezes, Rodrigo C., Marcelino, Andreza P.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.3390/tropicalmed9110277
Download full: https://arca.fiocruz.br/handle/icict/68382
Summary: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância de Leishmanioses. Rio de Janeiro, RJ, Brasil.
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spelling Santos, Isabela C. S.Avelar, Daniel Moreira deMiranda, Luciana F. C.Mello, Cintia X. deKeidel, LucasPimentel, Maria Inês F.Ventura, Luanna S.Fagundes, AlineSantos, Fernanda N.Oliveira, Liliane F. A.Santos, Shanna A.Pereira, Sandro AntonioMenezes, Rodrigo C.Marcelino, Andreza P.2025-02-03T17:22:32Z2025-02-03T17:22:32Z2024SANTOS, Isabela C S et al. Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling. Tropical Medicine and Infectious Disease, v. 9, n. 11, p. 277, 2024.2414-6366https://arca.fiocruz.br/handle/icict/6838210.3390/tropicalmed9110277engMDPI AGStandardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisa Médica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonose em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonose em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonose em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância de Leishmanioses. Rio de Janeiro, RJ, Brasil.The diagnosis of canine visceral leishmaniasis (CVL) presents a challenge due to a variety of non-specific clinical signs. The available tests have low sensitivity. This study aimed to standardize and evaluate the loop-mediated isothermal amplification technique with K26 target (K26-LAMP) for diagnosis of CVL in conjunctival swab (CS) DNA samples extracted through a silica column commercial kit (SW-kit) and boiling (SW-DB) and to compare sensitivity with conventional PCR (kDNA-cPCR) and quantitative real-time PCR (18S-qPCR). Clinical samples of CSs were collected from 54 dogs after reactive serology tests. Positive parasitological and/or histological tests were used as inclusion criteria for a sensitivity analysis. A total of 79.2% (43/54) of dogs without clinical signs or with mild, moderate, or severe clinical signs were included in the study. The sensitivity results of K26-LAMP, kDNA-cPCR, and 18S-qPCR were 72.1%, 81.4%, and 80.5% with the SW-kit and 97.2%, 95.2%, and 57.1% with SW-DB, respectively. In all techniques, the proportion of positives was higher in the group with severe clinical disease, with statistically significant differences in the K26-LAMP and 18S-qPCR techniques being seen with the SW-kit. The results obtained with LAMP for CS samples are promising and its performance is similar to other techniques.Canine visceral leishmaniasisConjunctival swabDiagnosisMolecular biologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/700a6057-4553-4a9f-b0b5-8ae483c13c72/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALStandardization and Evaluation of the LAMP Technique for the Diagnosis.pdfStandardization and Evaluation of the LAMP Technique for the Diagnosis.pdfapplication/pdf660696https://arca.fiocruz.br/bitstreams/094fd203-32b9-4cfb-ae32-0a78103c011f/downloade8c5693f70f4490b1461b53749c3640aMD52trueAnonymousREADTEXTStandardization and Evaluation of the LAMP Technique for the Diagnosis.pdf.txtStandardization and Evaluation of the LAMP Technique for the Diagnosis.pdf.txtExtracted texttext/plain52839https://arca.fiocruz.br/bitstreams/66f4c7ba-76c3-46e6-a5e9-fcc1354d454c/download2d89e6e2f074152eddbdbe8a427a408dMD57falseAnonymousREADTHUMBNAILStandardization and Evaluation of the LAMP Technique for the Diagnosis.pdf.jpgStandardization and Evaluation of the LAMP Technique for the Diagnosis.pdf.jpgGenerated Thumbnailimage/jpeg6038https://arca.fiocruz.br/bitstreams/89accb20-cd51-4ac9-8a10-3b82caeb0dd2/downloadd7b87775b859fc3a37ce131384a02586MD58falseAnonymousREADicict/683822025-07-29 19:10:30.482open.accessoai:arca.fiocruz.br:icict/68382https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-29T22:10:30Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.
title Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.
spellingShingle Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.
Santos, Isabela C. S.
Canine visceral leishmaniasis
Conjunctival swab
Diagnosis
Molecular biology
title_short Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.
title_full Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.
title_fullStr Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.
title_full_unstemmed Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.
title_sort Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.
author Santos, Isabela C. S.
author_facet Santos, Isabela C. S.
Avelar, Daniel Moreira de
Miranda, Luciana F. C.
Mello, Cintia X. de
Keidel, Lucas
Pimentel, Maria Inês F.
Ventura, Luanna S.
Fagundes, Aline
Santos, Fernanda N.
Oliveira, Liliane F. A.
Santos, Shanna A.
Pereira, Sandro Antonio
Menezes, Rodrigo C.
Marcelino, Andreza P.
author_role author
author2 Avelar, Daniel Moreira de
Miranda, Luciana F. C.
Mello, Cintia X. de
Keidel, Lucas
Pimentel, Maria Inês F.
Ventura, Luanna S.
Fagundes, Aline
Santos, Fernanda N.
Oliveira, Liliane F. A.
Santos, Shanna A.
Pereira, Sandro Antonio
Menezes, Rodrigo C.
Marcelino, Andreza P.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Isabela C. S.
Avelar, Daniel Moreira de
Miranda, Luciana F. C.
Mello, Cintia X. de
Keidel, Lucas
Pimentel, Maria Inês F.
Ventura, Luanna S.
Fagundes, Aline
Santos, Fernanda N.
Oliveira, Liliane F. A.
Santos, Shanna A.
Pereira, Sandro Antonio
Menezes, Rodrigo C.
Marcelino, Andreza P.
dc.subject.en.none.fl_str_mv Canine visceral leishmaniasis
Conjunctival swab
Diagnosis
Molecular biology
topic Canine visceral leishmaniasis
Conjunctival swab
Diagnosis
Molecular biology
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância de Leishmanioses. Rio de Janeiro, RJ, Brasil.
publishDate 2024
dc.date.issued.fl_str_mv 2024
dc.date.accessioned.fl_str_mv 2025-02-03T17:22:32Z
dc.date.available.fl_str_mv 2025-02-03T17:22:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Isabela C S et al. Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling. Tropical Medicine and Infectious Disease, v. 9, n. 11, p. 277, 2024.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/68382
dc.identifier.issn.none.fl_str_mv 2414-6366
dc.identifier.doi.none.fl_str_mv 10.3390/tropicalmed9110277
identifier_str_mv SANTOS, Isabela C S et al. Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling. Tropical Medicine and Infectious Disease, v. 9, n. 11, p. 277, 2024.
2414-6366
10.3390/tropicalmed9110277
url https://arca.fiocruz.br/handle/icict/68382
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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