Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis
| Main Author: | |
|---|---|
| Publication Date: | 2019 |
| Other Authors: | , , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1016/j.cmi.2018.12.027 |
| Download full: | https://arca.fiocruz.br/handle/icict/31587 |
Summary: | FAPESB e Brazil (SUS0024/ 2013). This study was financed in part by the Coordenaç~ao de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - Finance Code 001. |
| id |
CRUZ_d56f3c44682309e10c8c8ac5fc47c5d4 |
|---|---|
| oai_identifier_str |
oai:arca.fiocruz.br:icict/31587 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Canário, A.Queiroz, M.Cunha, G.Cavalcante, T.Riesz, V.Sharma, RohitNoronha, Almério deCorreia, T.Barral Netto, ManoelBarral, Aldina Maria PradoCunha, Antonio Ricardo KhouriOliveira, Viviane Sampaio Boaventura de2019-02-12T18:30:42Z2019-02-12T18:30:42Z2019CANÁRIO, A. et al. Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis. Clinical Microbiology and Infection, 2019.1198-743Xhttps://arca.fiocruz.br/handle/icict/3158710.1016/j.cmi.2018.12.027FAPESB e Brazil (SUS0024/ 2013). This study was financed in part by the Coordenaç~ao de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - Finance Code 001.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Pós-Graduação em Ciências da Saúde. Salvador, BA, Brasil / Hospital Santa Izabel. Santa Casa de Misericórdia da Bahia. Serviço de Otorrinolaringologia. Salvador, BA, Brazil.Hospital Santa Izabel. Santa Casa de Misericórdia da Bahia. Serviço de Otorrinolaringologia. Salvador, BA, Brazil.Hospital Santa Izabel. Santa Casa de Misericórdia da Bahia. Serviço de Otorrinolaringologia. Salvador, BA, Brazil.Hospital Santa Izabel. Santa Casa de Misericórdia da Bahia. Serviço de Otorrinolaringologia. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Pós-Graduação em Ciências da Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Pós-Graduação em Ciências da Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Pós-Graduação em Ciências da Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Pós-Graduação em Ciências da Saúde. Salvador, BA, Brasil / Hospital Santa Izabel. Santa Casa de Misericórdia da Bahia. Serviço de Otorrinolaringologia. Salvador, BA, Brazil.We aimed to detect Leishmania DNA carriage in nasal mucosa of individuals with cutaneous leishmaniasis (CL) caused by Leishmania (Viannia) braziliensis. Methods: A cross-sectional study was performed in all individuals with CL without nasal lesions (n ¼ 153) attended within 2 years in an endemic area of L. (Viannia) braziliensis in Bahia (Brazil). An otorhinolaryngologist assessed the clinical status of the nasal mucosa by anterior rhinoscopy and endoscopic examinations. Swab samples were collected for parasite DNA detection by PCR from all individuals before standard treatment for leishmaniasis. A second evaluation 3 months after treatment was performed to assess clinical outcomes. Results: Parasite DNA was detected in 7.8% (12/153) of clinically healthy nasal mucosa of individuals with CL. Interestingly, DNA was more frequently identified in individuals with more skin lesions (median 1.5, interquartile range (IQR) 1e3.5 versus 1.0, IQR 1e1.5; p 0.044), or larger injuries (median 2.7, IQR 2e3.8 versus 1.6, IQR 1e2.5; p 0.013). Additionally, the disease of those individuals with positive PCR evolved more frequently to unusual forms of leishmaniasis (recidiva cutis and disseminated) (45.5% (5/11) versus 11.5% (14/122); p 0.009), and required more cycles of treatment to reach clinical cure (median 2, IQR 1e4 versus 1, IQR 1e2; p 0.05). Conclusion: These findings suggest an early parasite tropism to nasal mucosa in L. (Viannia) braziliensis infection and a clinical phenotype of CL cases associated with parasite DNA in nasal mucosa. Future studies should evaluate whether PCR of nasal swab samples could serve as a prognostic tool for individuals at risk of mucocutaneous leishmaniasis.engElsevierLeishmaniose cutâneaLeishmania braziliensisLeishmaniose mucosaCotonete nasalReação em cadeia da polimeraseCutaneous leishmaniasisLeishmania braziliensisMucosal leishmaniasisNasal swabPolymerase chain reactionPresence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/798d8a99-6ddb-419c-9a79-82ddf226650a/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALCanário, A. Presence of parasite DNA in clinically...2019.pdfCanário, A. Presence of parasite DNA in clinically...2019.pdfapplication/pdf248161https://arca.fiocruz.br/bitstreams/ea7c1c52-214d-4864-b8a5-f3f0d3021384/downloadd71cb01c750c9ac331f7e6511eef667aMD52trueAnonymousREADTEXTCanário, A. Presence of parasite DNA in clinically...2019.pdf.txtCanário, A. Presence of parasite DNA in clinically...2019.pdf.txtExtracted texttext/plain22186https://arca.fiocruz.br/bitstreams/d7822c3b-be37-412e-a131-8799b559419c/download322ca3fa1756f3faacfc7fa84be5b700MD55falseAnonymousREADTHUMBNAILCanário, A. Presence of parasite DNA in clinically...2019.pdf.jpgCanário, A. Presence of parasite DNA in clinically...2019.pdf.jpgGenerated Thumbnailimage/jpeg6002https://arca.fiocruz.br/bitstreams/559d8102-af6d-4aa6-abdd-3e74e65c50d6/download87c90b70a718b4a49ad82aac6b4cf8c5MD56falseAnonymousREADicict/315872025-07-30 01:33:54.641open.accessoai:arca.fiocruz.br:icict/31587https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T04:33:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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 |
| dc.title.none.fl_str_mv |
Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis |
| title |
Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis |
| spellingShingle |
Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis Canário, A. Leishmaniose cutânea Leishmania braziliensis Leishmaniose mucosa Cotonete nasal Reação em cadeia da polimerase Cutaneous leishmaniasis Leishmania braziliensis Mucosal leishmaniasis Nasal swab Polymerase chain reaction |
| title_short |
Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis |
| title_full |
Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis |
| title_fullStr |
Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis |
| title_full_unstemmed |
Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis |
| title_sort |
Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis |
| author |
Canário, A. |
| author_facet |
Canário, A. Queiroz, M. Cunha, G. Cavalcante, T. Riesz, V. Sharma, Rohit Noronha, Almério de Correia, T. Barral Netto, Manoel Barral, Aldina Maria Prado Cunha, Antonio Ricardo Khouri Oliveira, Viviane Sampaio Boaventura de |
| author_role |
author |
| author2 |
Queiroz, M. Cunha, G. Cavalcante, T. Riesz, V. Sharma, Rohit Noronha, Almério de Correia, T. Barral Netto, Manoel Barral, Aldina Maria Prado Cunha, Antonio Ricardo Khouri Oliveira, Viviane Sampaio Boaventura de |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Canário, A. Queiroz, M. Cunha, G. Cavalcante, T. Riesz, V. Sharma, Rohit Noronha, Almério de Correia, T. Barral Netto, Manoel Barral, Aldina Maria Prado Cunha, Antonio Ricardo Khouri Oliveira, Viviane Sampaio Boaventura de |
| dc.subject.other.none.fl_str_mv |
Leishmaniose cutânea Leishmania braziliensis Leishmaniose mucosa Cotonete nasal Reação em cadeia da polimerase |
| topic |
Leishmaniose cutânea Leishmania braziliensis Leishmaniose mucosa Cotonete nasal Reação em cadeia da polimerase Cutaneous leishmaniasis Leishmania braziliensis Mucosal leishmaniasis Nasal swab Polymerase chain reaction |
| dc.subject.en.none.fl_str_mv |
Cutaneous leishmaniasis Leishmania braziliensis Mucosal leishmaniasis Nasal swab Polymerase chain reaction |
| description |
FAPESB e Brazil (SUS0024/ 2013). This study was financed in part by the Coordenaç~ao de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - Finance Code 001. |
| publishDate |
2019 |
| dc.date.accessioned.fl_str_mv |
2019-02-12T18:30:42Z |
| dc.date.available.fl_str_mv |
2019-02-12T18:30:42Z |
| dc.date.issued.fl_str_mv |
2019 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
CANÁRIO, A. et al. Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis. Clinical Microbiology and Infection, 2019. |
| dc.identifier.uri.fl_str_mv |
https://arca.fiocruz.br/handle/icict/31587 |
| dc.identifier.issn.none.fl_str_mv |
1198-743X |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.cmi.2018.12.027 |
| identifier_str_mv |
CANÁRIO, A. et al. Presence of parasite DNA in clinically unaffected nasal mucosa during cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis. Clinical Microbiology and Infection, 2019. 1198-743X 10.1016/j.cmi.2018.12.027 |
| url |
https://arca.fiocruz.br/handle/icict/31587 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://arca.fiocruz.br/bitstreams/798d8a99-6ddb-419c-9a79-82ddf226650a/download https://arca.fiocruz.br/bitstreams/ea7c1c52-214d-4864-b8a5-f3f0d3021384/download https://arca.fiocruz.br/bitstreams/d7822c3b-be37-412e-a131-8799b559419c/download https://arca.fiocruz.br/bitstreams/559d8102-af6d-4aa6-abdd-3e74e65c50d6/download |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 d71cb01c750c9ac331f7e6511eef667a 322ca3fa1756f3faacfc7fa84be5b700 87c90b70a718b4a49ad82aac6b4cf8c5 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1839716382073159680 |