Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis
| Main Author: | |
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| Publication Date: | 2007 |
| Other Authors: | , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1186/cc5783 |
| Download full: | https://arca.fiocruz.br/handle/icict/40892 |
Summary: | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. |
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Bozza, Fernando A.Salluh, Jorge I.Japiassú, André M.Soares, MarcioAssis, Edson F.Gomes, Rachel N.Bozza, Marcelo T.Faria Neto, Hugo Caire C.Bozza, Patrícia T.2020-04-20T18:03:46Z2020-04-20T18:03:46Z2007BOZZA, Fernando A. et al. Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis. Critical Care, v. 11, n. 2, p.1-8, Apr. 2007.1364-8535https://arca.fiocruz.br/handle/icict/4089210.1186/cc57831466-609XengBMCCitocinasMarcadoresSepseAnálise multiplexSeveridade da doençaCytokine profilesMarkersSisease severitySepsisMultiplex analysis03 Saúde e Bem-EstarCytokine profiles as markers of disease severity in sepsis: a multiplex analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.Hospital Barra D'Or. Unidade Barra da Tijuca, Rio de Janeiro, RJ, Brasil / Instituto Nacional do Câncer. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil / Hospital Quinta D'Or. Jacarepaguá, RJ, Brasil.Instituto Nacional do Câncer. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacologia. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Introduction The current shortage of accurate and readily available, validated biomarkers of disease severity in sepsis is an important limitation when attempting to stratify patients into homogeneous groups, in order to study pathogenesis or develop therapeutic interventions. The aim of the present study was to determine the cytokine profile in plasma of patients with severe sepsis by using a multiplex system for simultaneous detection of 17 cytokines. Methods This was a prospective cohort study conducted in four tertiary hospitals. A total of 60 patients with a recent diagnosis of severe sepsis were included. Plasma samples were collected for measurement of cytokine concentrations. A multiplex analysis was performed to evaluate levels of 17 cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, interferon-γ, granulocyte colony-stimulating factor [G-CSF], granulocyte-macrophage colony-stimulating factor, monocyte chemoattractant protein [MCP]-1, macrophage inflammatory protein-1 and tumour necrosis factor-α). Cytokine concentrations were related to the presence of severe sepsis or septic shock, the severity and evolution of organ failure, and early and late mortality. Results Concentrations of IL-1β, IL-6, IL-7, IL-8, IL-10, IL-13, interferon-γ, MCP-1 and tumour necrosis factor-α were significantly higher in septic shock patients than in those with severe sepsis. Cytokine concentrations were associated with severity and evolution of organ dysfunction. With regard to the severity of organ dysfunction on day 1, IL-8 and MCP-1 exhibited the best correlation with Sequential Organ Failure Assessment score. In addition, IL-6, IL-8 and G-CSF concentrations during the first 24 hours were predictive of worsening organ dysfunction or failure of organ dysfunction to improve on day three. In terms of predicting mortality, the cytokines IL-1β, IL-4, IL-6, IL-8, MCP-1 and G-CSF had good accuracy for predicting early mortality (< 48 hours), and IL-8 and MCP-1 had the best accuracy for predicting mortality at 28 days. In multivariate analysis, only MCP-1 was independently associated with prognosis. Conclusion In this exploratory analysis we demonstrated that use of a multiple cytokine assay platform allowed identification of distinct cytokine profiles associated with sepsis severity, evolution of organ failure and death.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/bdc647d1-b0d9-4c01-863e-bb398425e5f6/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALEdsonFAssis_RachelGomes_etal_IOC_2007.pdfEdsonFAssis_RachelGomes_etal_IOC_2007.pdfapplication/pdf207466https://arca.fiocruz.br/bitstreams/ec8c4497-01e4-4bab-b215-99efdde598da/download12cee73f9412a70da18527eaed333fd4MD52trueAnonymousREADTEXTEdsonFAssis_RachelGomes_etal_IOC_2007.pdf.txtEdsonFAssis_RachelGomes_etal_IOC_2007.pdf.txtExtracted texttext/plain48799https://arca.fiocruz.br/bitstreams/506caa66-2d45-4f5a-8d71-c86efcdbd163/download91a84796f55af8e42c2beccca0f11798MD55falseAnonymousREADTHUMBNAILEdsonFAssis_RachelGomes_etal_IOC_2007.pdf.jpgEdsonFAssis_RachelGomes_etal_IOC_2007.pdf.jpgGenerated Thumbnailimage/jpeg6266https://arca.fiocruz.br/bitstreams/78f16b31-5550-447f-8765-3242ba6bbb61/downloadd350124f29f2e71540f59ee1d23e0404MD56falseAnonymousREADicict/408922025-07-29 20:44:45.264open.accessoai:arca.fiocruz.br:icict/40892https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-29T23:44:45Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.none.fl_str_mv |
Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis |
| title |
Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis |
| spellingShingle |
Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis Bozza, Fernando A. Citocinas Marcadores Sepse Análise multiplex Severidade da doença Cytokine profiles Markers Sisease severity Sepsis Multiplex analysis 03 Saúde e Bem-Estar |
| title_short |
Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis |
| title_full |
Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis |
| title_fullStr |
Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis |
| title_full_unstemmed |
Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis |
| title_sort |
Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis |
| author |
Bozza, Fernando A. |
| author_facet |
Bozza, Fernando A. Salluh, Jorge I. Japiassú, André M. Soares, Marcio Assis, Edson F. Gomes, Rachel N. Bozza, Marcelo T. Faria Neto, Hugo Caire C. Bozza, Patrícia T. |
| author_role |
author |
| author2 |
Salluh, Jorge I. Japiassú, André M. Soares, Marcio Assis, Edson F. Gomes, Rachel N. Bozza, Marcelo T. Faria Neto, Hugo Caire C. Bozza, Patrícia T. |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bozza, Fernando A. Salluh, Jorge I. Japiassú, André M. Soares, Marcio Assis, Edson F. Gomes, Rachel N. Bozza, Marcelo T. Faria Neto, Hugo Caire C. Bozza, Patrícia T. |
| dc.subject.other.none.fl_str_mv |
Citocinas Marcadores Sepse Análise multiplex Severidade da doença |
| topic |
Citocinas Marcadores Sepse Análise multiplex Severidade da doença Cytokine profiles Markers Sisease severity Sepsis Multiplex analysis 03 Saúde e Bem-Estar |
| dc.subject.en.none.fl_str_mv |
Cytokine profiles Markers Sisease severity Sepsis Multiplex analysis |
| dc.subject.ods.none.fl_str_mv |
03 Saúde e Bem-Estar |
| description |
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. |
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2007 |
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2007 |
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2020-04-20T18:03:46Z |
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2020-04-20T18:03:46Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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BOZZA, Fernando A. et al. Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis. Critical Care, v. 11, n. 2, p.1-8, Apr. 2007. |
| dc.identifier.uri.fl_str_mv |
https://arca.fiocruz.br/handle/icict/40892 |
| dc.identifier.issn.none.fl_str_mv |
1364-8535 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/cc5783 |
| dc.identifier.eissn.none.fl_str_mv |
1466-609X |
| identifier_str_mv |
BOZZA, Fernando A. et al. Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis. Critical Care, v. 11, n. 2, p.1-8, Apr. 2007. 1364-8535 10.1186/cc5783 1466-609X |
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BMC |
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BMC |
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