Characterization of Leptospira isolates from humans and the environment in Uruguay

Bibliographic Details
Main Author: Meny, Paulina
Publication Date: 2017
Other Authors: Menéndez, Clara, Quintero, Jair, Hernández, Elba, Ríos, Cristina, Balassiano, Ilana Teruszkin, Trindade, Camilla Nunes dos Reis, Vital Brazil, Juliana Magalhães, Ramos, Tatiane Mendes Varela, Ashfield, Natalia, Feble, Camila, Avila, Esthefani, Schelotto, Felipe, Varela, Gustavo
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/S1678-9946201759079
Download full: https://arca.fiocruz.br/handle/icict/24788
Summary: Universidad de la República. Uruguay.
id CRUZ_cb41d84d09ed6f9e808fabdf6b812ba9
oai_identifier_str oai:arca.fiocruz.br:icict/24788
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Meny, PaulinaMenéndez, ClaraQuintero, JairHernández, ElbaRíos, CristinaBalassiano, Ilana TeruszkinTrindade, Camilla Nunes dos ReisVital Brazil, Juliana MagalhãesRamos, Tatiane Mendes VarelaAshfield, NataliaFeble, CamilaAvila, EsthefaniSchelotto, FelipeVarela, Gustavo2018-02-09T14:02:04Z2018-02-09T14:02:04Z2017MENY, Paulina; et al. Characterization of Leptospira isolates from humans and the environment in Uruguay. Rev Inst Med Trop São Paulo, v.59: e79, 9p, 2017.0036-4665https://arca.fiocruz.br/handle/icict/2478810.1590/S1678-99462017590791678-9946engUniversidade de São PauloLeptospiroseHumanosUruguaiLeptospiraHuman isolatesMLVA-VNTR-PCRLeptospirosisEnvironment LeptospiraLeptospira interrogansLeptospira kirschneriL. meyeriUruguayCharacterization of Leptospira isolates from humans and the environment in Uruguayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose. WHO Collaborating Center for Leptospirosis. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose. WHO Collaborating Center for Leptospirosis. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose. WHO Collaborating Center for Leptospirosis. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose. WHO Collaborating Center for Leptospirosis. Rio de Janeiro, RJ, Brasil.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Laboratory diagnosis of human leptospirosis usually relies on indirect methods exploring specific immune response. Isolation and identification of the involved strains are cumbersome, but can provide biological resources for pathogenic studies and relevant information for guiding prevention and control measures. The aim of the research we are hereby reporting was the characterization of Leptospira isolates obtained from humans and the environment in Uruguay. Blood cultures were performed from early samples of 302 Uruguayan patients, mainly rural workers, and from 36 water samples taken from their living or working environments. Eight human isolates and seven environmental isolates were obtained and analyzed by end point Polymerase Chain Reaction (PCR), Multilocus Variable Number of Tandem Repeat Analysis (MLVA) and other molecular methods. Human isolates corresponded to several serogroups and serovars of Leptospira interrogans and Leptospira kirschneri species, probably reflecting the infection with similar involved Leptospira species and serovars of an extended animal reservoir in rural settings of the country, mostly dedicated to meat and dairy production. Culture-positive patients were older than usually affected workers, and presented signs and symptoms of severe illness. A high organic and circulating bacterial burden may explain an easier positive result from these workers' samples. Environmental isolates were mainly identified as Leptospira biflexa strains, with a single L. meyeri isolate of uncertain significance.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/9dcdd714-a635-4abb-bf23-93ef57d039c9/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALilana_balassiano_etal_IOC_2017.pdfilana_balassiano_etal_IOC_2017.pdfapplication/pdf363896https://arca.fiocruz.br/bitstreams/047a1ea9-c7e6-4e27-b2f2-1d2a9b693b81/download7d4f35dfa3e09d7c09bf4c73cbb5dccfMD52trueAnonymousREADTEXTilana_balassiano_etal_IOC_2017.pdf.txtilana_balassiano_etal_IOC_2017.pdf.txtExtracted texttext/plain41602https://arca.fiocruz.br/bitstreams/ce54c4e0-e7ea-4f0e-a8f4-d77c804977ed/download49aaa8a6d7620149ddec0884b7141979MD55falseAnonymousREADTHUMBNAILilana_balassiano_etal_IOC_2017.pdf.jpgilana_balassiano_etal_IOC_2017.pdf.jpgGenerated Thumbnailimage/jpeg5512https://arca.fiocruz.br/bitstreams/a6b0ef07-ab73-4a62-b34b-475656e4f1a9/downloadd4912828809f418cd389e64b239255c1MD56falseAnonymousREADicict/247882025-07-30 02:09:43.442open.accessoai:arca.fiocruz.br:icict/24788https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T05:09:43Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
dc.title.none.fl_str_mv Characterization of Leptospira isolates from humans and the environment in Uruguay
title Characterization of Leptospira isolates from humans and the environment in Uruguay
spellingShingle Characterization of Leptospira isolates from humans and the environment in Uruguay
Meny, Paulina
Leptospirose
Humanos
Uruguai
Leptospira
Human isolates
MLVA-VNTR-PCR
Leptospirosis
Environment Leptospira
Leptospira interrogans
Leptospira kirschneri
L. meyeri
Uruguay
title_short Characterization of Leptospira isolates from humans and the environment in Uruguay
title_full Characterization of Leptospira isolates from humans and the environment in Uruguay
title_fullStr Characterization of Leptospira isolates from humans and the environment in Uruguay
title_full_unstemmed Characterization of Leptospira isolates from humans and the environment in Uruguay
title_sort Characterization of Leptospira isolates from humans and the environment in Uruguay
author Meny, Paulina
author_facet Meny, Paulina
Menéndez, Clara
Quintero, Jair
Hernández, Elba
Ríos, Cristina
Balassiano, Ilana Teruszkin
Trindade, Camilla Nunes dos Reis
Vital Brazil, Juliana Magalhães
Ramos, Tatiane Mendes Varela
Ashfield, Natalia
Feble, Camila
Avila, Esthefani
Schelotto, Felipe
Varela, Gustavo
author_role author
author2 Menéndez, Clara
Quintero, Jair
Hernández, Elba
Ríos, Cristina
Balassiano, Ilana Teruszkin
Trindade, Camilla Nunes dos Reis
Vital Brazil, Juliana Magalhães
Ramos, Tatiane Mendes Varela
Ashfield, Natalia
Feble, Camila
Avila, Esthefani
Schelotto, Felipe
Varela, Gustavo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Meny, Paulina
Menéndez, Clara
Quintero, Jair
Hernández, Elba
Ríos, Cristina
Balassiano, Ilana Teruszkin
Trindade, Camilla Nunes dos Reis
Vital Brazil, Juliana Magalhães
Ramos, Tatiane Mendes Varela
Ashfield, Natalia
Feble, Camila
Avila, Esthefani
Schelotto, Felipe
Varela, Gustavo
dc.subject.other.none.fl_str_mv Leptospirose
Humanos
Uruguai
topic Leptospirose
Humanos
Uruguai
Leptospira
Human isolates
MLVA-VNTR-PCR
Leptospirosis
Environment Leptospira
Leptospira interrogans
Leptospira kirschneri
L. meyeri
Uruguay
dc.subject.en.none.fl_str_mv Leptospira
Human isolates
MLVA-VNTR-PCR
Leptospirosis
Environment Leptospira
Leptospira interrogans
Leptospira kirschneri
L. meyeri
Uruguay
description Universidad de la República. Uruguay.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-09T14:02:04Z
dc.date.available.fl_str_mv 2018-02-09T14:02:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MENY, Paulina; et al. Characterization of Leptospira isolates from humans and the environment in Uruguay. Rev Inst Med Trop São Paulo, v.59: e79, 9p, 2017.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/24788
dc.identifier.issn.none.fl_str_mv 0036-4665
dc.identifier.doi.none.fl_str_mv 10.1590/S1678-9946201759079
dc.identifier.eissn.none.fl_str_mv 1678-9946
identifier_str_mv MENY, Paulina; et al. Characterization of Leptospira isolates from humans and the environment in Uruguay. Rev Inst Med Trop São Paulo, v.59: e79, 9p, 2017.
0036-4665
10.1590/S1678-9946201759079
1678-9946
url https://arca.fiocruz.br/handle/icict/24788
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://arca.fiocruz.br/bitstreams/9dcdd714-a635-4abb-bf23-93ef57d039c9/download
https://arca.fiocruz.br/bitstreams/047a1ea9-c7e6-4e27-b2f2-1d2a9b693b81/download
https://arca.fiocruz.br/bitstreams/ce54c4e0-e7ea-4f0e-a8f4-d77c804977ed/download
https://arca.fiocruz.br/bitstreams/a6b0ef07-ab73-4a62-b34b-475656e4f1a9/download
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
7d4f35dfa3e09d7c09bf4c73cbb5dccf
49aaa8a6d7620149ddec0884b7141979
d4912828809f418cd389e64b239255c1
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1839716431225159680

Similar Items