Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition

Bibliographic Details
Main Author: Lima, Flávia Oliveira de
Publication Date: 2012
Other Authors: Alves, Vivian, Barbosa Filho, José Maria, Almeida, Jackson Roberto Guedes da Silva, Rodrigues, Luis Cezar, Soares, Milena Botelho Pereira, Villarreal, Cristiane Flora
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://arca.fiocruz.br/handle/icict/7210
Summary: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil
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spelling Lima, Flávia Oliveira deAlves, VivianBarbosa Filho, José MariaAlmeida, Jackson Roberto Guedes da SilvaRodrigues, Luis CezarSoares, Milena Botelho PereiraVillarreal, Cristiane Flora2013-10-25T18:58:31Z2013-10-25T18:58:31Z2012LIMA,F. O. et al. Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition. Phytotherapy Research, v. 27, p. 1557–1563, 2013.10.1002/ptr.4902https://arca.fiocruz.br/handle/icict/7210engJohn Wiley & Sons, LtdAntinociceptive effect of lupeol: evidence for a role of cytokines inhibitioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Estadual de Feira de Santana. Feira de Santana, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, BrasilUniversidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, BrasilUniversidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, BrasilThe present study investigates the antinociceptive properties of lupeol inmodels of inflammatory and post-operative pain, as well as its mechanisms of action. The effects of lupeol were tested against acetic acid-induced writhing, formalin test, carrageenan-induced hyperalgesia, and post-operative pain model. Cytokine levels were determined by ELISA. Mice motor performance was evaluated in the rota rod and open-field tests. Pre-treatment of mice with lupeol (5–100mg/kg IP) produced a dose-related inhibition of writhing in mice. The maximal antinociception produced by lupeol (60mg/kg) was unaffected in mice pre-treated with yohimbine (a2 adrenoceptor antagonist; 2mg/kg IP), L-arginine (substrate for nitric oxide synthase; 600mg/kg IP), glibenclamide (the KATP-channel blocker; 2mg/kg IP), and methysergide maleate (serotoninergic receptors antagonist; 5mg/kg IP). Furthermore, lupeol (25–100mg/kg) inhibited the late phase of formalin test. Pre-treatment with lupeol (50 and 100mg/kg) inhibited the hyperalgesia and the local increase in tumor necrosis factor-a (TNF-a) and interleukin-1b (IL-1b) levels induced by carrageenan. In contrast, lupeol did not inhibit the post-operative pain. Lupeol-treated mice did not show any motor performance alterations or apparent systemic toxicity. Our results demonstrate that lupeol has consistent antinociceptive properties during inflammatory pain, but not post-operative pain, acting through the inhibition of IL-1b and TNF-a production.Lonchocarpus araripensisLupeolAntinociceptionInflammatory painPost-operative painCytokinesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFlavia OL Antinociceptive effect....pdfFlavia OL Antinociceptive effect....pdfapplication/pdf237804https://arca.fiocruz.br/bitstreams/33a25ab0-fe91-42e1-a3e8-f1487391c945/download8fb1facba729d829d7e76b35d51b82f5MD51trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81867https://arca.fiocruz.br/bitstreams/c296afcd-24f8-43a6-bdd0-1b3366b5b614/download89d048e714e969a1adcda05fdb1978beMD52falseAnonymousREADTEXTFlavia OL Antinociceptive effect....pdf.txtFlavia OL Antinociceptive effect....pdf.txtExtracted texttext/plain37058https://arca.fiocruz.br/bitstreams/673cf974-41d3-44aa-8438-bbe807aeb3ce/download7d4be40673d056f78ad45cdea2e00a35MD56falseAnonymousREADTHUMBNAILFlavia OL Antinociceptive effect....pdf.jpgFlavia OL Antinociceptive effect....pdf.jpgGenerated Thumbnailimage/jpeg6219https://arca.fiocruz.br/bitstreams/fa655bb2-3da8-4654-adf9-356396df3861/downloadffb998f10fbdaa99f97df8d01415078cMD57falseAnonymousREADicict/72102025-07-29 21:35:07.463open.accessoai:arca.fiocruz.br:icict/7210https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T00:35:07Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
dc.title.none.fl_str_mv Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition
title Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition
spellingShingle Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition
Lima, Flávia Oliveira de
Lonchocarpus araripensis
Lupeol
Antinociception
Inflammatory pain
Post-operative pain
Cytokines
title_short Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition
title_full Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition
title_fullStr Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition
title_full_unstemmed Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition
title_sort Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition
author Lima, Flávia Oliveira de
author_facet Lima, Flávia Oliveira de
Alves, Vivian
Barbosa Filho, José Maria
Almeida, Jackson Roberto Guedes da Silva
Rodrigues, Luis Cezar
Soares, Milena Botelho Pereira
Villarreal, Cristiane Flora
author_role author
author2 Alves, Vivian
Barbosa Filho, José Maria
Almeida, Jackson Roberto Guedes da Silva
Rodrigues, Luis Cezar
Soares, Milena Botelho Pereira
Villarreal, Cristiane Flora
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima, Flávia Oliveira de
Alves, Vivian
Barbosa Filho, José Maria
Almeida, Jackson Roberto Guedes da Silva
Rodrigues, Luis Cezar
Soares, Milena Botelho Pereira
Villarreal, Cristiane Flora
dc.subject.en.none.fl_str_mv Lonchocarpus araripensis
Lupeol
Antinociception
Inflammatory pain
Post-operative pain
Cytokines
topic Lonchocarpus araripensis
Lupeol
Antinociception
Inflammatory pain
Post-operative pain
Cytokines
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2013-10-25T18:58:31Z
dc.date.available.fl_str_mv 2013-10-25T18:58:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA,F. O. et al. Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition. Phytotherapy Research, v. 27, p. 1557–1563, 2013.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/7210
dc.identifier.issn.none.fl_str_mv 10.1002/ptr.4902
identifier_str_mv LIMA,F. O. et al. Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition. Phytotherapy Research, v. 27, p. 1557–1563, 2013.
10.1002/ptr.4902
url https://arca.fiocruz.br/handle/icict/7210
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv John Wiley & Sons, Ltd
publisher.none.fl_str_mv John Wiley & Sons, Ltd
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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