APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease

Bibliographic Details
Main Author: Alladagbin, Dona Jeanne
Publication Date: 2022
Other Authors: Silva, Carlos Gustavo Regis da, Silva, Luciano Kalabric, Santos, Washington L. C. dos, Oliveira, Geraldo Gileno de Sá
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1186/s12882-022-02954-w
Download full: https://arca.fiocruz.br/handle/icict/55973
Summary: Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
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spelling Alladagbin, Dona JeanneSilva, Carlos Gustavo Regis daSilva, Luciano KalabricSantos, Washington L. C. dosOliveira, Geraldo Gileno de Sá2022-12-15T16:36:34Z2022-12-15T16:36:34Z2022ALLADAGBIN, Dona Jeanne et al. APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease. BMC Nephrology, v. 23, n. 329, p. 1-7, 2022.1471-2369https://arca.fiocruz.br/handle/icict/5597310.1186/s12882-022-02954-wFundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Molecular e Estrutural. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Molecular e Estrutural. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Molecular e Estrutural. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Molecular e Estrutural. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Molecular e Estrutural. Salvador, BA, Brasil.Two alleles (G1 and G2) of the apolipoprotein 1 gene (APOL1) predispose people of African descent to developing or accelerating the course of certain types of kidney disease. Population studies to determine the frequency of the G1 and G2 alleles are important to inform resource allocation by public health authorities. Traditionally, APOL1 genotyping is carried out in blood samples. However, sample collection, transport, and storage is cumbersome. Here we compared APOL1 genotyping in blood and buccal mucosa cell samples obtained from 23 individuals. Alleles G0 (wild), G1, and G2, as well as genotypes G0/G0, G0/G1, G1/G1, G0/G2, G1/G2, and G2/G2 were detected using both blood and buccal mucosa cells with 100% coincidence. Our data indicate that buccal mucosa cell samples may represent a suitable alternative to blood samples for APOL1 genotyping in the field.engBMCBuccal mucosa cellsSanguePCRsequenciamento de DNAApolipoproteína LHaplótiposBuccal mucosa cellsBloodPCRDNA sequencingApolipoprotein LHaplotypesSangueReação em Cadeia da PolimeraseSequenciamento de Nucleotídeos em Larga EscalaApolipoproteína L1HaplótiposAPOL1 genotyping via buccal mucosa cell samples to establish risk of kidney diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/96e83589-658e-4898-a326-4af4a501b632/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALAlladagn, D. J. APOL1....pdfAlladagn, D. J. 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dc.title.none.fl_str_mv APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease
title APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease
spellingShingle APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease
Alladagbin, Dona Jeanne
Buccal mucosa cells
Sangue
PCR
sequenciamento de DNA
Apolipoproteína L
Haplótipos
Buccal mucosa cells
Blood
PCR
DNA sequencing
Apolipoprotein L
Haplotypes
Sangue
Reação em Cadeia da Polimerase
Sequenciamento de Nucleotídeos em Larga Escala
Apolipoproteína L1
Haplótipos
title_short APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease
title_full APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease
title_fullStr APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease
title_full_unstemmed APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease
title_sort APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease
author Alladagbin, Dona Jeanne
author_facet Alladagbin, Dona Jeanne
Silva, Carlos Gustavo Regis da
Silva, Luciano Kalabric
Santos, Washington L. C. dos
Oliveira, Geraldo Gileno de Sá
author_role author
author2 Silva, Carlos Gustavo Regis da
Silva, Luciano Kalabric
Santos, Washington L. C. dos
Oliveira, Geraldo Gileno de Sá
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Alladagbin, Dona Jeanne
Silva, Carlos Gustavo Regis da
Silva, Luciano Kalabric
Santos, Washington L. C. dos
Oliveira, Geraldo Gileno de Sá
dc.subject.other.none.fl_str_mv Buccal mucosa cells
Sangue
PCR
sequenciamento de DNA
Apolipoproteína L
Haplótipos
topic Buccal mucosa cells
Sangue
PCR
sequenciamento de DNA
Apolipoproteína L
Haplótipos
Buccal mucosa cells
Blood
PCR
DNA sequencing
Apolipoprotein L
Haplotypes
Sangue
Reação em Cadeia da Polimerase
Sequenciamento de Nucleotídeos em Larga Escala
Apolipoproteína L1
Haplótipos
dc.subject.en.none.fl_str_mv Buccal mucosa cells
Blood
PCR
DNA sequencing
Apolipoprotein L
Haplotypes
dc.subject.decs.none.fl_str_mv Sangue
Reação em Cadeia da Polimerase
Sequenciamento de Nucleotídeos em Larga Escala
Apolipoproteína L1
Haplótipos
description Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-12-15T16:36:34Z
dc.date.available.fl_str_mv 2022-12-15T16:36:34Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALLADAGBIN, Dona Jeanne et al. APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease. BMC Nephrology, v. 23, n. 329, p. 1-7, 2022.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/55973
dc.identifier.issn.none.fl_str_mv 1471-2369
dc.identifier.doi.none.fl_str_mv 10.1186/s12882-022-02954-w
identifier_str_mv ALLADAGBIN, Dona Jeanne et al. APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease. BMC Nephrology, v. 23, n. 329, p. 1-7, 2022.
1471-2369
10.1186/s12882-022-02954-w
url https://arca.fiocruz.br/handle/icict/55973
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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