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Antinociceptive properties of Micrurus lemniscatus venom

Bibliographic Details
Main Author: Santos, Gisele Graça Leite dos
Publication Date: 2012
Other Authors: Silva, Luciana Lyra Casais e, Soares, Milena Botelho Pereira, Villarreal, Cristiane Flora
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: doi.org/10.1016/j.toxicon.2012.07.003
Download full: https://arca.fiocruz.br/handle/icict/7235
Summary: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling Santos, Gisele Graça Leite dosSilva, Luciana Lyra Casais eSoares, Milena Botelho PereiraVillarreal, Cristiane Flora2013-11-14T18:05:41Z2013-11-14T18:05:41Z2012SANTOS, G. G. L. dos et al. Antinociceptive properties of Micrurus lemniscatus venom. Toxicon, v. 60, p. 1005–1012, 2012.0041-0101https://arca.fiocruz.br/handle/icict/7235doi.org/10.1016/j.toxicon.2012.07.003engElsevierAntinociceptive properties of Micrurus lemniscatus venominfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, BrasilThe therapeutic potential of snake venoms for pain control has been previously demonstrated. In the present study, the antinociceptive effects of Micrurus lemniscatus venom (MlV) were investigated in experimental models of pain. The antinociceptive activity of MIV was evaluated using the writhing, formalin, and tail flick tests. Mice motor performance was assessed in the rota rod and open field tests. In a screening test for new antinociceptive substances – the writhing test – oral administration of MlV (19.7–1600 mg/ kg) produced significant antinociceptive effect. The venom (1600 mg/kg) also inhibited both phases of the formalin test, confirming the antinociceptive activity. The administration of MlV (1600 mg/kg) did not cause motor impairment in the rota rod and open field tests, which excluded possible non-specific muscle relaxant or sedative effects of the venom. The MIV (177–1600 mg/kg) also increases the tail flick latency response, indicating a central antinociceptive effect for the venom. In this test, the MlV-induced antinociceptive effect was long-lasting and higher than that of morphine, an analgesic considered the gold standard. In another set of experiments, the mechanisms involved in the venom-induced antinociception were investigated through the use of pharmacological antagonists. The MlV (1600 mg/kg) antinociceptive effect was prevented by naloxone (5 mg/kg), a nonselective opioid receptor antagonist, suggesting that this effect is mediated by activation of opioid receptors. In addition, the pre-treatment with the m-opioid receptor antagonist CTOP (1 mg/kg) blocked the venom antinociceptive effect, while the k-opioid receptor antagonist nor-BNI (0.5 mg/kg) or the d-opioid receptor antagonist naltrindole (3 mg/kg) only partially reduced the venom-induced antinociception. The present study demonstrates, for the first time, that oral administration of M. lemniscatus venom, at doses that did not induce any motor performance alteration, produced potent and long-lasting antinociceptive effect mediated by activation of opioid receptors.Micrurus lemniscatusPainAnalgesicAntinociceptionVenomOpioid systeminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALGisele Graça Leite dos Santos Antinociceptive....pdfGisele Graça Leite dos Santos Antinociceptive....pdfapplication/pdf619941https://arca.fiocruz.br/bitstreams/4d3bdc5a-fc84-475f-aaf8-aa53307037b7/downloadb5a51fdefe0012c7691d360620260921MD51trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://arca.fiocruz.br/bitstreams/16b00dee-9951-4b0c-8107-fbcf381fa725/download7d48279ffeed55da8dfe2f8e81f3b81fMD52falseAnonymousREADTEXTGisele Graça Leite dos Santos Antinociceptive....pdf.txtGisele Graça Leite dos Santos Antinociceptive....pdf.txtExtracted texttext/plain36105https://arca.fiocruz.br/bitstreams/748e35de-d88f-48f3-800f-3c645ba63586/downloadcd6bec1983d49d2a5591e04971d4b198MD56falseAnonymousREADTHUMBNAILGisele Graça Leite dos Santos Antinociceptive....pdf.jpgGisele Graça Leite dos Santos Antinociceptive....pdf.jpgGenerated Thumbnailimage/jpeg5282https://arca.fiocruz.br/bitstreams/caf81d65-691d-4cd1-b22f-17903f3e0649/downloade36239e0a5c2daa43ec0f4c83e478b2bMD57falseAnonymousREADicict/72352025-07-29 23:01:03.368open.accessoai:arca.fiocruz.br:icict/7235https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T02:01:03Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
dc.title.none.fl_str_mv Antinociceptive properties of Micrurus lemniscatus venom
title Antinociceptive properties of Micrurus lemniscatus venom
spellingShingle Antinociceptive properties of Micrurus lemniscatus venom
Santos, Gisele Graça Leite dos
Micrurus lemniscatus
Pain
Analgesic
Antinociception
Venom
Opioid system
title_short Antinociceptive properties of Micrurus lemniscatus venom
title_full Antinociceptive properties of Micrurus lemniscatus venom
title_fullStr Antinociceptive properties of Micrurus lemniscatus venom
title_full_unstemmed Antinociceptive properties of Micrurus lemniscatus venom
title_sort Antinociceptive properties of Micrurus lemniscatus venom
author Santos, Gisele Graça Leite dos
author_facet Santos, Gisele Graça Leite dos
Silva, Luciana Lyra Casais e
Soares, Milena Botelho Pereira
Villarreal, Cristiane Flora
author_role author
author2 Silva, Luciana Lyra Casais e
Soares, Milena Botelho Pereira
Villarreal, Cristiane Flora
author2_role author
author
author
dc.contributor.author.fl_str_mv Santos, Gisele Graça Leite dos
Silva, Luciana Lyra Casais e
Soares, Milena Botelho Pereira
Villarreal, Cristiane Flora
dc.subject.en.none.fl_str_mv Micrurus lemniscatus
Pain
Analgesic
Antinociception
Venom
Opioid system
topic Micrurus lemniscatus
Pain
Analgesic
Antinociception
Venom
Opioid system
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2013-11-14T18:05:41Z
dc.date.available.fl_str_mv 2013-11-14T18:05:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, G. G. L. dos et al. Antinociceptive properties of Micrurus lemniscatus venom. Toxicon, v. 60, p. 1005–1012, 2012.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/7235
dc.identifier.issn.none.fl_str_mv 0041-0101
dc.identifier.doi.none.fl_str_mv doi.org/10.1016/j.toxicon.2012.07.003
identifier_str_mv SANTOS, G. G. L. dos et al. Antinociceptive properties of Micrurus lemniscatus venom. Toxicon, v. 60, p. 1005–1012, 2012.
0041-0101
doi.org/10.1016/j.toxicon.2012.07.003
url https://arca.fiocruz.br/handle/icict/7235
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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