Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile
| Main Author: | |
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| Publication Date: | 2024 |
| Other Authors: | , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.3390/brainsci14010090 |
| Download full: | https://arca.fiocruz.br/handle/icict/64025 |
Summary: | Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior Nível (CAPES) Pro Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Instituto Nacional de Neurociência Translacional |
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Lima, Irlã SantosSoares, Érica NovaesNonaka, Carolina Kymie VasquesSouza, Bruno Solano de FreitasSantos, Balbino Lino dosCosta, Silvia Lima2024-05-13T16:45:01Z2024-05-13T16:45:01Z2024LIMA, Irlã Santos et al. Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile. Brain Sciences, v. 14, n. 1, p. 1-14, 2024.2076-3425https://arca.fiocruz.br/handle/icict/6402510.3390/brainsci14010090Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior Nível (CAPES) Pro Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Instituto Nacional de Neurociência TranslacionalUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Neuroquímica e Biologia Celular. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Neuroquímica e Biologia Celular. Salvador, BA, Brasil.Instituto D’Or de Pesquisa e Ensino (IDOR). Centro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, BrasilInstituto D’Or de Pesquisa e Ensino (IDOR). Centro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Neuroquímica e Biologia Celular. Salvador, BA, Brasil / Universidade Federal do Vale do São Francisco. Faculdade de Enfermagem. Petrolina, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Neuroquímica e Biologia Celular. Salvador, BA, Brasil / Instituto Nacional de Neurociências da Tradução (INNT). Rio de Janeiro, BA, Brasil.Glioblastoma (GBM) is the most aggressive and treatment-resistant brain tumor. In the GBM microenvironment, interaction with microglia is associated with the dysregulation of cytokines, chemokines, and miRNAs, contributing to angiogenesis, proliferation, anti-apoptosis, and chemoresistance. The flavonoid rutin can inhibit glioma cell growth associated with microglial activation and production of pro-inflammatory mediators by mechanisms that are still poorly understood. The present study investigated the effect of rutin on viability, regulation of miRNA-125b, and the STAT3 expression in GBM cells, as well as the effects on the modulation of the inflammatory profile and STAT3 expression in microglia during indirect interaction with GBM cells. Human GL15-GBM cells and human C20 microglia were treated or not with rutin for 24 h. Rutin (30–50 μM) significantly reduced the viability of GL15 cells; however, it did not affect the viability of microglia. Rutin (30 μM) significantly reduced the expression of miRNA-125b in the cells and secretome and STAT3 expression. Microglia submitted to the conditioned medium from GBM cells treated with rutin showed reactive morphology associated with reduced expression of IL-6, TNF, and STAT3. These results reiterate the anti-glioma effects of the flavonoid, which may also modulate microglia towards a more responsive anti-tumor phenotype, constituting a promising molecule for adjuvant therapy to GBM.engMDPIGlioblastomaMiRNA-125bSTAT3Citocinas inflamatóriasRutinaGlioblastomaMiRNA-125bSTAT3Inflammatory cytokinesRutinGlioblastomaCitocinasRutinaFlavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profileinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/4ce096b4-14a0-44ab-8dba-15042e4e6b2d/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALLima, S. L. Flavonoid_Rutin_....pdfLima, S. L. 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Flavonoid_Rutin_....pdf.jpgGenerated Thumbnailimage/jpeg6005https://arca.fiocruz.br/bitstreams/5a83b192-6478-425c-bb49-e46aac69cc09/downloade8141863df5d908a0579a5b8c11a8dedMD56falseAnonymousREADicict/640252025-07-30 02:32:29.382open.accessoai:arca.fiocruz.br:icict/64025https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T05:32:29Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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 |
| dc.title.none.fl_str_mv |
Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile |
| title |
Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile |
| spellingShingle |
Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile Lima, Irlã Santos Glioblastoma MiRNA-125b STAT3 Citocinas inflamatórias Rutina Glioblastoma MiRNA-125b STAT3 Inflammatory cytokines Rutin Glioblastoma Citocinas Rutina |
| title_short |
Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile |
| title_full |
Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile |
| title_fullStr |
Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile |
| title_full_unstemmed |
Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile |
| title_sort |
Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile |
| author |
Lima, Irlã Santos |
| author_facet |
Lima, Irlã Santos Soares, Érica Novaes Nonaka, Carolina Kymie Vasques Souza, Bruno Solano de Freitas Santos, Balbino Lino dos Costa, Silvia Lima |
| author_role |
author |
| author2 |
Soares, Érica Novaes Nonaka, Carolina Kymie Vasques Souza, Bruno Solano de Freitas Santos, Balbino Lino dos Costa, Silvia Lima |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Lima, Irlã Santos Soares, Érica Novaes Nonaka, Carolina Kymie Vasques Souza, Bruno Solano de Freitas Santos, Balbino Lino dos Costa, Silvia Lima |
| dc.subject.other.none.fl_str_mv |
Glioblastoma MiRNA-125b STAT3 Citocinas inflamatórias Rutina |
| topic |
Glioblastoma MiRNA-125b STAT3 Citocinas inflamatórias Rutina Glioblastoma MiRNA-125b STAT3 Inflammatory cytokines Rutin Glioblastoma Citocinas Rutina |
| dc.subject.en.none.fl_str_mv |
Glioblastoma MiRNA-125b STAT3 Inflammatory cytokines Rutin |
| dc.subject.decs.none.fl_str_mv |
Glioblastoma Citocinas Rutina |
| description |
Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior Nível (CAPES) Pro Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Instituto Nacional de Neurociência Translacional |
| publishDate |
2024 |
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2024-05-13T16:45:01Z |
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2024-05-13T16:45:01Z |
| dc.date.issued.fl_str_mv |
2024 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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LIMA, Irlã Santos et al. Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile. Brain Sciences, v. 14, n. 1, p. 1-14, 2024. |
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https://arca.fiocruz.br/handle/icict/64025 |
| dc.identifier.issn.none.fl_str_mv |
2076-3425 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/brainsci14010090 |
| identifier_str_mv |
LIMA, Irlã Santos et al. Flavonoid rutin presented anti-glioblastoma activity related to the modulation of onco miRNA-125b expression and STAT3 signaling and impact on microglia inflammatory profile. Brain Sciences, v. 14, n. 1, p. 1-14, 2024. 2076-3425 10.3390/brainsci14010090 |
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https://arca.fiocruz.br/handle/icict/64025 |
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eng |
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MDPI |
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MDPI |
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