Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2024 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://arca.fiocruz.br/handle/icict/70554 |
Resumo: | Wethank Dr. Danae Lopes Francisco for collaboration with MEVimagesandMSc.KellyCristina Manhani for the support in quantification analyses. We also grateful to pharmaceutical companies Laboratório Cristália (Brazil), Lipid Ingredients (Brazil), and DFE Pharma (Germany) for kindly providing azithromycin dehydrated, soybean lecithin S75, and lactose monohydrated LH206, respectively. |
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Sani, Alison Tatiana MadridRocha, Khellida Loiane V. RamosSarcinelli, Michelle AlvaresChaves, Marcelo Henrique da CunhaRocha, Helvécio Vinícius AntunesLéo, PatríciaCerize, Natália Neto PereiraZanin, Maria Helena AmbrosioFeitosa, Valker AraujoYagui, Carlota de Oliveira Rangel2025-05-22T15:43:11Z2025-05-22T15:43:11Z2024SANI, Alison Tatiana Madrid et al. Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles. Journal of Pharmacy & Pharmaceutical Sciences. v.14, n. 27, p.1-15, 2024.1482-1826https://arca.fiocruz.br/handle/icict/70554Wethank Dr. Danae Lopes Francisco for collaboration with MEVimagesandMSc.KellyCristina Manhani for the support in quantification analyses. We also grateful to pharmaceutical companies Laboratório Cristália (Brazil), Lipid Ingredients (Brazil), and DFE Pharma (Germany) for kindly providing azithromycin dehydrated, soybean lecithin S75, and lactose monohydrated LH206, respectively.TheCOVID-19pandemichasraisedconcernregardingrespiratory systemdiseases and oral inhalation stands out as an attractive non-invasive route of administration for pulmonary diseases such as chronic bronchitis, cystic fibrosis, COVID-19 and community-acquiredpneumonia.Inthiscontext,weencapsulatedazithromycin in polycaprolactone nanoparticles functionalized with phospholipids rich in dipalmitoylphosphatidylcholine and further produced a fine powder formulation by spray drying with monohydrated lactose. Nanoparticles obtained by the emulsion/solvent diffusion-evaporation technique exhibited a mean hydrodynamic diameter around 195–228 nm with a narrow monomodal size distribution (PdI < 0.2). Nanoparticle dispersions were spray-dried at different inlet temperatures, atomizing air-flow,aspirator air flow,andfeedrate,using lactoseasa drying aid, resulting in a maximal process yield of 63% and an encapsulation efficiency of 83%. Excipients and the dry powder formulations were characterized in terms of morphology, chemical structure, thermal analyses and particle size by SEM, FTIR, DSC/TGA and laser light diffraction. The results indicated spherical particles with 90% at 4.06 µm or below, an adequate size for pulmonary delivery. Aerosolization performance in a NGI confirmed good aerodynamic properties. Microbiological assays showed that the formulation preserves AZM antimicrobial effect against Staphylococcus aureus and Streptococcus pneumoniae strains, with halos above 18 mm. In addition, no formulation-related cytotoxicity was observed against the human cell lines BEAS-2B (lung epithelial), HUVEC (endothelial) and HFF1 (fibroblasts). Overall, the approach described here allows the production of AZM-PCL nanoparticles incorporated into inhalable microparticles, enabling more efficient pulmonary therapy of lung infections.The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. The authors declare that this study received support from Laboratório Cristália (Brazil), Lipid Ingredients (Brazil), and DFE Pharma (Germany). The companies were not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication. Financial support from CAPES (88887.506666/ 2020-00) is greatly acknowledged.Technological Research Institute. Bionanomanufacturing Center. São Paulo, SP, Brasil / University of São Paulo. Department of Biochemical and Pharmaceutical Technology. SãoPaulo, SP, Brasil.Technological Research Institute. Bionanomanufacturing Center. São Paulo, SP, Brasil / University of São Paulo. Department of Biochemical and Pharmaceutical Technology. SãoPaulo, SP, Brasil.Fundação Oswaldo Cruz. Farmanguinhos . Laboratório de Micro e Nanotecnologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Farmanguinhos . Laboratório de Micro e Nanotecnologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Farmanguinhos . Laboratório de Micro e Nanotecnologia. Rio de Janeiro, RJ, Brasil.Technological Research Institute. Bionanomanufacturing Center. São Paulo, SP, Brasil.Technological Research Institute. Bionanomanufacturing Center. São Paulo, SP, Brasil.Technological Research Institute. Bionanomanufacturing Center. São Paulo, SP, Brasil.University of São Paulo. Department of Biochemical and Pharmaceutical Technology. SãoPaulo, SP, Brasil.University of São Paulo. Department of Biochemical and Pharmaceutical Technology. 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| dc.title.none.fl_str_mv |
Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles |
| title |
Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles |
| spellingShingle |
Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles Sani, Alison Tatiana Madrid Spray-drying Polycaprolactone Azithromycin Dry powder inhaler Pulmonary diseases COVID-19 Secagem por Atomização Azitromicina Inaladores de Pó Seco Pneumopatias COVID-19 |
| title_short |
Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles |
| title_full |
Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles |
| title_fullStr |
Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles |
| title_full_unstemmed |
Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles |
| title_sort |
Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles |
| author |
Sani, Alison Tatiana Madrid |
| author_facet |
Sani, Alison Tatiana Madrid Rocha, Khellida Loiane V. Ramos Sarcinelli, Michelle Alvares Chaves, Marcelo Henrique da Cunha Rocha, Helvécio Vinícius Antunes Léo, Patrícia Cerize, Natália Neto Pereira Zanin, Maria Helena Ambrosio Feitosa, Valker Araujo Yagui, Carlota de Oliveira Rangel |
| author_role |
author |
| author2 |
Rocha, Khellida Loiane V. Ramos Sarcinelli, Michelle Alvares Chaves, Marcelo Henrique da Cunha Rocha, Helvécio Vinícius Antunes Léo, Patrícia Cerize, Natália Neto Pereira Zanin, Maria Helena Ambrosio Feitosa, Valker Araujo Yagui, Carlota de Oliveira Rangel |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sani, Alison Tatiana Madrid Rocha, Khellida Loiane V. Ramos Sarcinelli, Michelle Alvares Chaves, Marcelo Henrique da Cunha Rocha, Helvécio Vinícius Antunes Léo, Patrícia Cerize, Natália Neto Pereira Zanin, Maria Helena Ambrosio Feitosa, Valker Araujo Yagui, Carlota de Oliveira Rangel |
| dc.subject.en.none.fl_str_mv |
Spray-drying Polycaprolactone Azithromycin Dry powder inhaler Pulmonary diseases COVID-19 |
| topic |
Spray-drying Polycaprolactone Azithromycin Dry powder inhaler Pulmonary diseases COVID-19 Secagem por Atomização Azitromicina Inaladores de Pó Seco Pneumopatias COVID-19 |
| dc.subject.decs.none.fl_str_mv |
Secagem por Atomização Azitromicina Inaladores de Pó Seco Pneumopatias COVID-19 |
| description |
Wethank Dr. Danae Lopes Francisco for collaboration with MEVimagesandMSc.KellyCristina Manhani for the support in quantification analyses. We also grateful to pharmaceutical companies Laboratório Cristália (Brazil), Lipid Ingredients (Brazil), and DFE Pharma (Germany) for kindly providing azithromycin dehydrated, soybean lecithin S75, and lactose monohydrated LH206, respectively. |
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2024 |
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2024 |
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2025-05-22T15:43:11Z |
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2025-05-22T15:43:11Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SANI, Alison Tatiana Madrid et al. Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles. Journal of Pharmacy & Pharmaceutical Sciences. v.14, n. 27, p.1-15, 2024. |
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https://arca.fiocruz.br/handle/icict/70554 |
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1482-1826 |
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SANI, Alison Tatiana Madrid et al. Development of a dry powder formulation for pulmonary delivery of azithromycin-loaded nanoparticles. Journal of Pharmacy & Pharmaceutical Sciences. v.14, n. 27, p.1-15, 2024. 1482-1826 |
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CSPS |
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