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Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis

Bibliographic Details
Main Author: Zahner, Viviane
Publication Date: 2013
Other Authors: Silva, Ana Carolina Telles de Carvalho e, Moraes, Gabriela Pinhel de, Mcintosh, Douglas, Filippis, Ivano de
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: http://dx.doi.org/10.1590/S0074-02762013000100011
Download full: https://arca.fiocruz.br/handle/icict/8549
http://dx.doi.org/10.1590/S0074-02762013000100011
Summary: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.
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spelling Zahner, VivianeSilva, Ana Carolina Telles de Carvalho eMoraes, Gabriela Pinhel deMcintosh, DouglasFilippis, Ivano de2014-10-08T17:14:23Z2014-10-08T17:14:23Z2013ZAHNER, V. et al. Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 1, p. 65-72, fev. 2013.0074-0276https://arca.fiocruz.br/handle/icict/8549http://dx.doi.org/10.1590/S0074-02762013000100011engInstituto Oswaldo CruzExtended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Departamento de Parasitologia Animal. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Multiple locus sequence typing (MLST) was undertaken to extend the genetic characterization of 29 isolates of Bacillus cereus and Bacillus thuringiensis previously characterized in terms of presence/absence of sequences encoding virulence factors and via variable number tandem repeat (VNTR). Additional analysis involved polymerase chain reaction for the presence of sequences (be, cytK, inA, pag, lef, cya and cap), encoding putative virulence factors, not investigated in the earlier study. MLST analysis ascribed novel and unique sequence types to each of the isolates. A phylogenetic tree was constructed from a single sequence of 2,838 bp of concatenated loci sequences. The strains were not monophyletic by analysis of any specific housekeeping gene or virulence characteristic. No clear association in relation to source of isolation or to genotypic profile based on the presence or absence of putative virulence genes could be identified. Comparison of VNTR profiling with MLST data suggested a correlation between these two methods of genetic analysis. In common with the majority of previous studies, MLST was unable to provide clarification of the basis for pathogenicity among members of the B. cereus complex. Nevertheless, our application of MLST served to reinforce the notion that B. cereus and B. thuringiensis should be considered as the same species.Bacillus cereusMultiple Locus Sequence TypingVirulence FactorsBacillus cereusTipagem de Sequências MultilocusFatores de VirulênciaBacillus thuringiensisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://arca.fiocruz.br/bitstreams/87c6f607-26ab-4a00-8df5-364a70871623/download7d48279ffeed55da8dfe2f8e81f3b81fMD51falseAnonymousREADORIGINALMem Inst Oswaldo Cruz_108_65-72.pdfMem Inst Oswaldo Cruz_108_65-72.pdfapplication/pdf549982https://arca.fiocruz.br/bitstreams/4a86374f-8879-4a7d-a7ae-187f9e8b8c81/download151d2fbabfd18e15838943595b5b9b1eMD52trueAnonymousREADTEXTMem Inst Oswaldo Cruz_108_65-72.pdf.txtMem Inst Oswaldo Cruz_108_65-72.pdf.txtExtracted texttext/plain42323https://arca.fiocruz.br/bitstreams/3e4c0694-95ee-46f1-8c45-6a8556314084/download58426a82e249a215fad84df20ad2a18dMD55falseAnonymousREADTHUMBNAILMem Inst Oswaldo Cruz_108_65-72.pdf.jpgMem Inst Oswaldo Cruz_108_65-72.pdf.jpgGenerated Thumbnailimage/jpeg5726https://arca.fiocruz.br/bitstreams/83a3b840-b964-4deb-a61c-11c532921615/download985d327c77ebdde0539b7b97a61105e9MD56falseAnonymousREADicict/85492025-07-29 22:46:48.523open.accessoai:arca.fiocruz.br:icict/8549https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T01:46:48Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
dc.title.none.fl_str_mv Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
title Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
spellingShingle Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
Zahner, Viviane
Bacillus cereus
Multiple Locus Sequence Typing
Virulence Factors
Bacillus cereus
Tipagem de Sequências Multilocus
Fatores de Virulência
Bacillus thuringiensis
title_short Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
title_full Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
title_fullStr Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
title_full_unstemmed Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
title_sort Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
author Zahner, Viviane
author_facet Zahner, Viviane
Silva, Ana Carolina Telles de Carvalho e
Moraes, Gabriela Pinhel de
Mcintosh, Douglas
Filippis, Ivano de
author_role author
author2 Silva, Ana Carolina Telles de Carvalho e
Moraes, Gabriela Pinhel de
Mcintosh, Douglas
Filippis, Ivano de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Zahner, Viviane
Silva, Ana Carolina Telles de Carvalho e
Moraes, Gabriela Pinhel de
Mcintosh, Douglas
Filippis, Ivano de
dc.subject.en.none.fl_str_mv Bacillus cereus
Multiple Locus Sequence Typing
Virulence Factors
topic Bacillus cereus
Multiple Locus Sequence Typing
Virulence Factors
Bacillus cereus
Tipagem de Sequências Multilocus
Fatores de Virulência
Bacillus thuringiensis
dc.subject.decs.none.fl_str_mv Bacillus cereus
Tipagem de Sequências Multilocus
Fatores de Virulência
Bacillus thuringiensis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-10-08T17:14:23Z
dc.date.available.fl_str_mv 2014-10-08T17:14:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ZAHNER, V. et al. Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 1, p. 65-72, fev. 2013.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/8549
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.1590/S0074-02762013000100011
identifier_str_mv ZAHNER, V. et al. Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 1, p. 65-72, fev. 2013.
0074-0276
url https://arca.fiocruz.br/handle/icict/8549
http://dx.doi.org/10.1590/S0074-02762013000100011
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
publisher.none.fl_str_mv Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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