Genome-wide transposon mutagenesis in pathogenic Leptospira species

Bibliographic Details
Main Author: Murray, Gerald L.
Publication Date: 2009
Other Authors: Morel, Viviane, Cerqueira, Gustavo Maia de, Croda, Julio Henrique Rosa, Srikram, Amporn, Henry, Rebekah, Ko, Albert Icksang, Dellagostin, Odir Antonio, Bulach, Dieter M., Sermswan, Rasana W., Adler, Ben, Picardeau, Mathieu
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1128/IAI.01293-08
Download full: https://arca.fiocruz.br/handle/icict/12331
Summary: Monash University. Australian Bacterial Pathogenesis Program. Department of Microbiology. Clayton, Australia
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spelling Murray, Gerald L.Morel, VivianeCerqueira, Gustavo Maia deCroda, Julio Henrique RosaSrikram, AmpornHenry, RebekahKo, Albert IcksangDellagostin, Odir AntonioBulach, Dieter M.Sermswan, Rasana W.Adler, BenPicardeau, Mathieu2015-11-30T13:31:07Z2015-11-30T13:31:07Z2009MURRAY, G. L. et al. Genome-wide transposon mutagenesis in pathogenic Leptospira species. Infection and Immunity, v. 77, n. 2, p. 810-816, 2009.1098-5522https://arca.fiocruz.br/handle/icict/1233110.1128/IAI.01293-08engAmerican Society for MicrobiologyGenome-wide transposon mutagenesis in pathogenic Leptospira speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMonash University. Australian Bacterial Pathogenesis Program. Department of Microbiology. Clayton, AustraliaInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, FranceInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, France / Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, France / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilKhon Kaen University. Faculty of Medicine. Melioidosis Research Center. Khon Kaen, ThailandMonash University. Australian Bacterial Pathogenesis Program. Department of Microbiology. Clayton, AustraliaFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. NY, New YorkUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilMonash University. Australian Bacterial Pathogenesis Program. Department of Microbiology. Clayton, AustraliaKhon Kaen University. Faculty of Medicine. Department of Biochemistry. Khon Kaen, ThailandMonash University. Australian Bacterial Pathogenesis Program. Department of Microbiology. Clayton, Australia / Monash University. Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics. Department of Microbiology. Clayton, AustraliaInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, FranceLeptospira interrogans is the most common cause of leptospirosis in humans and animals. Genetic analysis of L. interrogans has been severely hindered by a lack of tools for genetic manipulation. Recently we developed the mariner-based transposon Himar1 to generate the first defined mutants in L. interrogans. In this study, a total of 929 independent transposon mutants were obtained and the location of insertion determined. Of these mutants, 721 were located in the protein coding regions of 551 different genes. While sequence analysis of transposon insertion sites indicated that transposition occurred in an essentially random fashion in the genome, 25 unique transposon mutants were found to exhibit insertions into genes encoding 16S or 23S rRNAs, suggesting these genes are insertional hot spots in the L. interrogans genome. In contrast, loci containing notionally essential genes involved in lipopolysaccharide and heme biosynthesis showed few transposon insertions. The effect of gene disruption on the virulence of a selected set of defined mutants was investigated using the hamster model of leptospirosis. Two attenuated mutants with disruptions in hypothetical genes were identified, thus validating the use of transposon mutagenesis for the identification of novel virulence factors in L. interrogans. This library provides a valuable resource for the study of gene function in L. interrogans. Combined with the genome sequences of L. interrogans, this provides an opportunity to investigate genes that contribute to pathogenesis and will provide a better understanding of the biology of L. interrogans.Elementos de DNA Transponíveis/genéticaGenoma Bacteriano/genéticaLeptospira/fisiologiaMutagênese Insercional/métodosAnimaisMapeamento CromossômicoCromossomos BacterianosCricetinaeBiblioteca GênicaLeptospira/genéticaLeptospirose/imunologiaLeptospirose/microbiologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/999468c6-d1fb-4a2b-8f9a-d6977eda7b7f/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALMurray GL and genome wide transposon mutagenesis....pdfMurray GL and genome wide transposon mutagenesis....pdfapplication/pdf651958https://arca.fiocruz.br/bitstreams/c9b7237e-9836-4f07-8232-ffe9d1ee3c29/download35490ffc78e3469e4b232ecf861c4c0fMD52trueAnonymousREADTEXTMurray GL and genome wide transposon mutagenesis....pdf.txtMurray GL and genome wide transposon mutagenesis....pdf.txtExtracted texttext/plain45679https://arca.fiocruz.br/bitstreams/eaada2e6-a51a-4af9-a240-6f2497885f77/downloadc585c5cadd90944a6b10fb1067a684a4MD55falseAnonymousREADTHUMBNAILMurray GL and genome wide transposon mutagenesis....pdf.jpgMurray GL and genome wide transposon mutagenesis....pdf.jpgGenerated Thumbnailimage/jpeg6129https://arca.fiocruz.br/bitstreams/1fa1a7c9-aab9-4d29-baab-d84b263b4921/download60312d3ad5368e503ab87545033aa130MD56falseAnonymousREADicict/123312025-07-30 00:38:42.226open.accessoai:arca.fiocruz.br:icict/12331https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T03:38:42Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Genome-wide transposon mutagenesis in pathogenic Leptospira species
title Genome-wide transposon mutagenesis in pathogenic Leptospira species
spellingShingle Genome-wide transposon mutagenesis in pathogenic Leptospira species
Murray, Gerald L.
Elementos de DNA Transponíveis/genética
Genoma Bacteriano/genética
Leptospira/fisiologia
Mutagênese Insercional/métodos
Animais
Mapeamento Cromossômico
Cromossomos Bacterianos
Cricetinae
Biblioteca Gênica
Leptospira/genética
Leptospirose/imunologia
Leptospirose/microbiologia
title_short Genome-wide transposon mutagenesis in pathogenic Leptospira species
title_full Genome-wide transposon mutagenesis in pathogenic Leptospira species
title_fullStr Genome-wide transposon mutagenesis in pathogenic Leptospira species
title_full_unstemmed Genome-wide transposon mutagenesis in pathogenic Leptospira species
title_sort Genome-wide transposon mutagenesis in pathogenic Leptospira species
author Murray, Gerald L.
author_facet Murray, Gerald L.
Morel, Viviane
Cerqueira, Gustavo Maia de
Croda, Julio Henrique Rosa
Srikram, Amporn
Henry, Rebekah
Ko, Albert Icksang
Dellagostin, Odir Antonio
Bulach, Dieter M.
Sermswan, Rasana W.
Adler, Ben
Picardeau, Mathieu
author_role author
author2 Morel, Viviane
Cerqueira, Gustavo Maia de
Croda, Julio Henrique Rosa
Srikram, Amporn
Henry, Rebekah
Ko, Albert Icksang
Dellagostin, Odir Antonio
Bulach, Dieter M.
Sermswan, Rasana W.
Adler, Ben
Picardeau, Mathieu
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Murray, Gerald L.
Morel, Viviane
Cerqueira, Gustavo Maia de
Croda, Julio Henrique Rosa
Srikram, Amporn
Henry, Rebekah
Ko, Albert Icksang
Dellagostin, Odir Antonio
Bulach, Dieter M.
Sermswan, Rasana W.
Adler, Ben
Picardeau, Mathieu
dc.subject.decs.none.fl_str_mv Elementos de DNA Transponíveis/genética
Genoma Bacteriano/genética
Leptospira/fisiologia
Mutagênese Insercional/métodos
Animais
Mapeamento Cromossômico
Cromossomos Bacterianos
Cricetinae
Biblioteca Gênica
Leptospira/genética
Leptospirose/imunologia
Leptospirose/microbiologia
topic Elementos de DNA Transponíveis/genética
Genoma Bacteriano/genética
Leptospira/fisiologia
Mutagênese Insercional/métodos
Animais
Mapeamento Cromossômico
Cromossomos Bacterianos
Cricetinae
Biblioteca Gênica
Leptospira/genética
Leptospirose/imunologia
Leptospirose/microbiologia
description Monash University. Australian Bacterial Pathogenesis Program. Department of Microbiology. Clayton, Australia
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2015-11-30T13:31:07Z
dc.date.available.fl_str_mv 2015-11-30T13:31:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MURRAY, G. L. et al. Genome-wide transposon mutagenesis in pathogenic Leptospira species. Infection and Immunity, v. 77, n. 2, p. 810-816, 2009.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/12331
dc.identifier.issn.none.fl_str_mv 1098-5522
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.01293-08
identifier_str_mv MURRAY, G. L. et al. Genome-wide transposon mutagenesis in pathogenic Leptospira species. Infection and Immunity, v. 77, n. 2, p. 810-816, 2009.
1098-5522
10.1128/IAI.01293-08
url https://arca.fiocruz.br/handle/icict/12331
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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